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Team:TU Darmstadt/Materials/NBT
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== NBT == | == NBT == | ||
=== About === | === About === | ||
| - | Nitro blue tetrazolium chloride is a marker used in the reaction catalysed by the [https://2012.igem.org/Team:TU_Darmstadt/Materials#Phosphatases alkaline phosphatase] | + | Nitro blue tetrazolium chloride is a marker used in the reaction catalysed by the [https://2012.igem.org/Team:TU_Darmstadt/Materials#Phosphatases alkaline phosphatase]. The colored product reveals where the antibody is bound, or can be used in immunofluorescence.<sup>[1]</sup> |
=== Application === | === Application === | ||
* 158 mg Nitro blue tetrazolium chloride (75 mg/mL NBT) | * 158 mg Nitro blue tetrazolium chloride (75 mg/mL NBT) | ||
Latest revision as of 21:00, 26 September 2012
Contents |
NBT
About
Nitro blue tetrazolium chloride is a marker used in the reaction catalysed by the alkaline phosphatase. The colored product reveals where the antibody is bound, or can be used in immunofluorescence.[1]
Application
- 158 mg Nitro blue tetrazolium chloride (75 mg/mL NBT)
- 1,475 mL DMF
- 0,632 mL dist. water
References
[1] http://en.wikipedia.org/wiki/Nitro_blue_tetrazolium_chloride
