Team:Goettingen/week9-3

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Language: <img height="20", src="http://www.patrickreinke.de/igem/eng.jpg"> English, <img height="20", src="http://www.patrickreinke.de/igem/deu.jpg"> <a href="https://2012.igem.org/Team:Goettingen/main_deu">Deutsch</a> <br>
 
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<h2><b>VX_Y </b></h2><br>
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<h2><b>V06_25 </b></h2><br>
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<b>Titel</b><br>
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<b>Ligation of promoterX_TAR_QC constructs into pSB1C3</b><br>
<ul>
<ul>
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<li>Experiment: <br>hier text reinschreiben</li>
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<li>Experiment: <br>8 different promoter_TAR_QC constructs (for details see <a href ="https://2012.igem.org/Team:Goettingen/week7-3">here</a>) were ligated into the vector pSB1C3 according to <a href ="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>. Subsequently <i>E. coli</i> &Delta;<i>tar</i> were transformed with the generated constructs, plated on CM plates and incubated at 37 °C overnight.</li>
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<a href="#top">&uarr; Return to top</a>
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<h2><b>V06_28 </b></h2><br>
 +
<b>V06_28_1 Preparation of pSB1C3_TAR_QC</b><br>
 +
<ul>
 +
<li>Experiment: <br>The vector pSB1C3 was prepped using the peqGOLD Plasmid Miniprep Kit (Peqlab) following the user maual. Elution in 50 µL elution buffer. </li>
 +
</ul>
<br>
<br>
 +
<b>V06_28_2 Test digest of pSB1C3_TAR_QC</b><br>
 +
<ul>
 +
<li>Experiment: <br>A test digest of prepped pSB1C3_TAR_QC was performed according to <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a> using <i>Xba</i>I and <i>Pst</i>I. This should verify that the insertion of promoterX_TAR_QC into pSB1C3 has actually succeeded. </li>
 +
</ul>
 +
<ul>
 +
<li>Observations & Results: <br>The corresponding Agarose gel showed bands at the expected fragment sizes (1721 bp for TAR_QC and 2048 bp for the vector backbone).
 +
Plasmids were labeled:<br></html>
 +
{| class="goetable"
 +
|-
 +
!Name !! Anderson promoter!! BioBrick
 +
|-
 +
| pSB1C3_TAR_QC_18C || J23100 ||  K777001
 +
|-
 +
| pSB1C3_TAR_QC_18K|| J23104 ||  K777002
 +
|-
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| pSB1C3_TAR_QC_18M|| J23105 ||  K777003
 +
|-
 +
| pSB1C3_TAR_QC_18O|| J23106 ||  K777004
 +
|-
 +
| pSB1C3_TAR_QC_2G|| J23109 ||  K777005
 +
|-
 +
| pSB1C3_TAR_QC_20E|| J23112 ||  K777006
 +
|-
 +
| pSB1C3_TAR_QC_20G|| J23113 ||  K777007
 +
|-
 +
| pSB1C3_TAR_QC_20I|| J23114 ||  K777008
 +
|-
 +
|}
 +
<html>
 +
These parts are submitted as BioBricks (see our <a href="https://2012.igem.org/Team:Goettingen/iGEM/Parts">submitted parts</a>).
 +
</li>
 +
</ul>
 +
<br></td></tr>
 +
</table>
<br>
<br>
 +
<a href="https://2012.igem.org/Team:Goettingen/Notebook">Back to overview</a><br>
<a href="https://2012.igem.org/Team:Goettingen/Notebook">Back to overview</a><br>
<br>
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<b>Important pages</b>:<br>
 
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<a href="https://2012.igem.org/Team:Goettingen">Home</a>;
 
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<a href="https://2012.igem.org/Team:Goettingen/Team">Team</a>;
 
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<a href="https://igem.org/Team.cgi?year=2012">Official Team Profile</a>;
 
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<a href="https://2012.igem.org/Team:Goettingen/Project">Project</a>;
 
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<a href="https://2012.igem.org/Team:Goettingen/Parts">Parts submitted to the Registry</a>;
 
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<a href="https://2012.igem.org/Team:Goettingen/Modeling">Modeling</a>;
 
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<a href="https://2012.igem.org/Team:Goettingen/Notebook">Notebook</a>;
 
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<a href="https://2012.igem.org/Team:Goettingen/Saftey">Saftey</a>;
 
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{{GoettingenFooter}}

Latest revision as of 23:41, 26 September 2012

Deutsch  / English 

#3 Chemoreceptor Library - 9th Week

Back to overview

V06_25


Ligation of promoterX_TAR_QC constructs into pSB1C3
  • Experiment:
    8 different promoter_TAR_QC constructs (for details see here) were ligated into the vector pSB1C3 according to protocol. Subsequently E. coli Δtar were transformed with the generated constructs, plated on CM plates and incubated at 37 °C overnight.


V06_28


V06_28_1 Preparation of pSB1C3_TAR_QC
  • Experiment:
    The vector pSB1C3 was prepped using the peqGOLD Plasmid Miniprep Kit (Peqlab) following the user maual. Elution in 50 µL elution buffer.

V06_28_2 Test digest of pSB1C3_TAR_QC
  • Experiment:
    A test digest of prepped pSB1C3_TAR_QC was performed according to protocol using XbaI and PstI. This should verify that the insertion of promoterX_TAR_QC into pSB1C3 has actually succeeded.
  • Observations & Results:
    The corresponding Agarose gel showed bands at the expected fragment sizes (1721 bp for TAR_QC and 2048 bp for the vector backbone). Plasmids were labeled:

    Name Anderson promoter BioBrick
    pSB1C3_TAR_QC_18C J23100 K777001
    pSB1C3_TAR_QC_18K J23104 K777002
    pSB1C3_TAR_QC_18M J23105 K777003
    pSB1C3_TAR_QC_18O J23106 K777004
    pSB1C3_TAR_QC_2G J23109 K777005
    pSB1C3_TAR_QC_20E J23112 K777006
    pSB1C3_TAR_QC_20G J23113 K777007
    pSB1C3_TAR_QC_20I J23114 K777008

    These parts are submitted as BioBricks (see our submitted parts).



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