Team:LMU-Munich/Spore Coat Proteins
From 2012.igem.org
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+ | [[File:LMU Glow Spore2 cutII.jpg|620px|link=]] | ||
- | [[File: | + | [[File:SporeCoat.png|100px|right|link=]] |
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+ | =='''Sporo'''beads - What Protein Do ''You'' Want to Display?== | ||
+ | <br> | ||
+ | <p align="justify">'''Sporo'''beads are the first generation of ''Bacillus subtilis'' endospores displaying a protein of our choice on their outermost layer, the spore crust. In future '''Sporo'''beads could serve as a platform for protein display and thus be used for numerous versatile [https://2012.igem.org/Team:LMU-Munich/Application applications]. Our goal was to show that ''B. subtilis'' spores have the ability to do so. As a [https://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins/result proof of principle] we successfully fused GFP to the spore crust and obtained fluorescence with microscopy.</p> | ||
- | == | + | <div class="box"> |
+ | ===Scientific Background=== | ||
+ | {| "width=100%" style="text-align:center;" style="align:right"| | ||
+ | |<p align="justify">Introduction to ''B. subtilis'' spores and their use in our project</p> | ||
+ | |[[File:Imamura, 2011 & McKenney, 2010.png|200px|right|link=Team:LMU-Munich/Spore_Coat_Proteins/Background]] | ||
+ | |- | ||
+ | ! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Spore_Coat_Proteins/Background]] | ||
+ | |} | ||
+ | </div> | ||
+ | <div class="box"> | ||
+ | ===Cloning Strategy=== | ||
+ | {| "width=100%" style="text-align:center;" style="align:right"| | ||
+ | |<p align="justify">Cloning strategy to create different variants of our ''' Sporo'''beads</p> | ||
+ | |[[File:Final construct.png|200px|link=Team:LMU-Munich/Spore_Coat_Proteins/cloning]] | ||
+ | |- | ||
+ | ! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Spore_Coat_Proteins/cloning]] | ||
+ | |} | ||
+ | </div> | ||
- | <p align="justify"> | + | <div class="box"> |
+ | ===GFP as a Proof of Principle=== | ||
+ | {| "width=100%" style="text-align:center;" style="align:right"| | ||
+ | |<p align="justify">Main results of the various constructs that were created to find the best one!</p> | ||
+ | |[[File:LMU Firstspore.jpg|200px|right|link=Team:LMU-Munich/Spore_Coat_Proteins/result]] | ||
+ | |- | ||
+ | ! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Spore_Coat_Proteins/result]] | ||
+ | |} | ||
+ | </div> | ||
- | + | <div class="box"> | |
+ | ===Laccases as functional enzymes=== | ||
+ | {| "width=100%" style="text-align:center;" style="align:right"| | ||
+ | |<p align="justify">Creation of functional Laccase-''' Sporo'''beads</p> | ||
+ | |[[File:Final construct.png|200px|link=Team:LMU-Munich/Spore_Coat_Proteins/laccases]] | ||
+ | |- | ||
+ | ! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Spore_Coat_Proteins/laccases]] | ||
+ | |} | ||
+ | </div> | ||
- | < | + | <div class="box"> |
- | + | ===Purification Methods=== | |
- | <p align="justify"> | + | {| "width=100%" style="text-align:center;" style="align:right"| |
- | + | |<p align="justify">Description of the different purification methods of the spores</p> | |
- | [[File: | + | |[[File:Treatments.png|200px|right|link=Team:LMU-Munich/Spore_Coat_Proteins/purification]] |
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|- | |- | ||
- | | | + | ! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Spore_Coat_Proteins/purification]] |
- | < | + | |} |
- | < | + | </div> |
- | + | <br> | |
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- | < | + | <br> |
- | + | <div class="box"> | |
- | + | ====Project Navigation==== | |
+ | {| width="100%" align="center" style="text-align:center;" | ||
+ | |[[File:Bacilluss_Intro.png|100px|link=Team:LMU-Munich/Bacillus_Introduction]] | ||
+ | |[[File:BacillusBioBrickBox.png|100px|link=Team:LMU-Munich/Bacillus_BioBricks]] | ||
+ | |[[File:SporeCoat.png|100px|link=Team:LMU-Munich/Spore_Coat_Proteins]] | ||
+ | |[[File:GerminationSTOP.png|100px|link=Team:LMU-Munich/Germination_Stop]] | ||
+ | |- | ||
+ | |[[Team:LMU-Munich/Bacillus_Introduction|<font size="2">'''''Bacillus'''''<BR>Intro</font>]] | ||
+ | |[[Team:LMU-Munich/Bacillus_BioBricks|<font size="2" face="verdana">'''''Bacillus'''''<BR>'''B'''io'''B'''rick'''B'''ox</font>]] | ||
+ | |[[Team:LMU-Munich/Spore_Coat_Proteins|<font size="2" face="verdana">'''Sporo'''beads</font>]] | ||
+ | |[[Team:LMU-Munich/Germination_Stop|<font size="2" face="verdana">'''Germination'''<BR>STOP</font>]] | ||
+ | |} | ||
+ | </div> | ||
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{{:Team:LMU-Munich/Templates/Page Footer}} | {{:Team:LMU-Munich/Templates/Page Footer}} |
Latest revision as of 13:44, 14 November 2012
![Team-LMU eppis.resized.jpg](/wiki/images/c/cb/Team-LMU_eppis.resized.jpg)
The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".
[ more news ]
![Sporenfreunde](https://static.igem.org/mediawiki/2012/e/e3/Team_LMU_Sporenfreunde.png)
![SporeCoat.png](/wiki/images/c/c1/SporeCoat.png)
Sporobeads - What Protein Do You Want to Display?
Sporobeads are the first generation of Bacillus subtilis endospores displaying a protein of our choice on their outermost layer, the spore crust. In future Sporobeads could serve as a platform for protein display and thus be used for numerous versatile applications. Our goal was to show that B. subtilis spores have the ability to do so. As a proof of principle we successfully fused GFP to the spore crust and obtained fluorescence with microscopy.
GFP as a Proof of Principle
Main results of the various constructs that were created to find the best one! | |
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Bacillus Intro | Bacillus BioBrickBox | Sporobeads | Germination STOP |