Team:LMU-Munich

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|<font size="5">'''Opening ''Bacillus subtilis'' and its unique biology to the iGEM world'''</font>
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|[[File:NEXT.png|100px|link=Team:LMU-Munich/Abstract]]
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|'''Bead'''zillus - For a guided tour through the most important information about our project and results, klick [[Team:LMU-Munich/Abstract|here]].
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<a href="https://2012.igem.org/Team:LMU-Munich/Project" style="left: 276px; top: 40px; width: 271px; height:265px;"><img style="left: -276px; top: -40px" alt="" src="http://wrtlprnft.ath.cx/igem2012_home0.png"></a>
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<a href="https://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins" style="left: 130px; top: 50px; width: 146px; height:145px;"><img style="left: -130px; top: -50px" alt="" src="http://wrtlprnft.ath.cx/igem2012_home1.png"></a>
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<a href="https://2012.igem.org/Team:LMU-Munich/Germination_Stop" style="left: 166px; top: 196px; width: 110px; height:110px;"><img style="left: -166px; top: -196px" alt="" src="http://wrtlprnft.ath.cx/igem2012_home2.png"></a>
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<a href="https://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks" style="left: 55px; top: 160px; width: 110px; height:110px;"><img style="left: -55px; top: -160px" alt="" src="http://wrtlprnft.ath.cx/igem2012_home3.png"></a>
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|[[File:LMU_Why_BS.png|100px|link=Team:LMU-Munich/Why_Beadzillus]]
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|Why is there a need for our Project [[Team:LMU-Munich/Why_Beadzillus|'''Bead'''zillus]]?
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|[[File:LMU_The_solution.png|100px|link=Team:LMU-Munich/Bacillus_Introduction]]
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|Why we choose to work with [[Team:LMU-Munich/Bacillus_Introduction|''Bacillus subtilis'']].
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|[[File:Requirements.png|95px|link=Team:LMU-Munich/Bacillus_BioBricks]]
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|To carry out our project we first to had to create a [[Team:LMU-Munich/Bacillus_BioBricks|<b><i>Bacillus</i>B</b>io<b>B</b>rick<b>B</b>ox]] and had to think over important [[Team:LMU-Munich/Germination_Stop|security issues]].
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|[[File:4-Sporobeads.png|95px|link=Team:LMU-Munich/Spore_Coat_Proteins]]
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|Finally, we succeeded in displaying proteins on endospores. Our [[Team:LMU-Munich/Spore_Coat_Proteins|'''Sporo'''beads]].
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|[[File:LMU 5-Application.png|90px|link=Team:LMU-Munich/Application]]
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|With our '''Sporo'''beads a lot of [[Team:LMU-Munich/Application|applications]] are imaginable.
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We chose to work with <i>Bacillus subtilis</i> to set new horizons and offer tools for this model organism to the <i>Escherichia coli</i>-dominated world of iGEM. Therefore, we created a <b><i>Bacillus</i>B</b>io<b>B</b>rick<b>B</b>ox (<b>BBBB</b>) composed of reporter genes, defined promoters, as well as reporter, expression, and empty vectors in BioBrick standard. <i>B. subtilis</i> naturally produces stress resistant endospores which can germinate in response to suitable environmental conditions. To highlight this unique feature using the <b>BBBB</b>, we developed <b>Sporo</b>beads. These are spores displaying fusion proteins on their surface. As a proof of principle, we fused GFP to the outermost layer. Expanding this idea, we designed a <b>Sporo</b>vector to easily create any <b>Sporo</b>bead imaginable. Because the <b>Sporo</b>beads must be biologically safe and stable vehicles, we prevented germination by knocking out involved genes and developed a <b>Suicide</b>switch turned on in case of germination. With the project <b>Bead</b>zillus, our team demonstrates the powerful nature of <i>B. subtilis</i>.
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We chose to work with [https://2012.igem.org/Team:LMU-Munich/Bacillus_Introduction <i>Bacillus subtilis</i>] to set new horizons and offer tools for this model organism to the <i>Escherichia coli</i>-dominated world of iGEM. Therefore, we created a [https://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks <b><i>Bacillus</i>B</b>io<b>B</b>rick<b>B</b>ox] (<b>B<sup>4</sup></b>) composed of reporter genes, defined promoters, as well as reporter, expression, and empty vectors in BioBrick standard. <i>B. subtilis</i> naturally produces stress-resistant endospores which can germinate in response to suitable environmental conditions. To highlight this unique feature using the <b>B<sup>4</sup></b>, we developed [https://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins <b>Sporo</b>beads]. These are spores displaying fusion proteins on their surface. As a proof of principle, we fused GFP to the outermost layer. Expanding this idea, we designed a [https://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks/Sporovector <b>Sporo</b>vector] to easily create any <b>Sporo</b>bead imaginable. Because the <b>Sporo</b>beads must be biologically safe and stable vehicles, we [https://2012.igem.org/Team:LMU-Munich/Germination_Stop prevented germination] by knocking out involved genes and developed a [https://2012.igem.org/Team:LMU-Munich/Germination_Stop <b>Suicide</b>switch] turned on in case of germination. With the project <b>Bead</b>zillus, our team demonstrates the powerful nature of <i>B. subtilis</i>.
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'''Project Navigation'''
 
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[[File:Bacilluss_Intro.png|100px|link=https://2012.igem.org/Team:LMU-Munich/Bacillus_Introduction]]
 
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[[Team:LMU-Munich/Bacillus_Introduction|''B. subtilis'' Intro]]
 
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[[File:BacillusBioBrickBox.png|100px|link=https://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks]]
 
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[[Team:LMU-Munich/Bacillus_BioBricks|'''''Bacillus''B'''io'''B'''rick'''B'''ox]]
 
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[[File:SporeCoat.png|100px|link=https://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins]]
 
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[[Team:LMU-Munich/Spore_Coat_Proteins|'''Sporo'''beads]]
 
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[[File:GerminationSTOP.png|90px|link=https://2012.igem.org/Team:LMU-Munich/Germination_Stop]]
 
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[[Team:LMU-Munich/Germination_Stop|'''Germination'''STOP]]
 
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====Team LMU Munich 2012====
====Team LMU Munich 2012====
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We are the iGEM team of the Ludwig-Maximilians-University (LMU) in Munich. The team consists of 10 dedicated members: 7 students and our 3 advisors.
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We are the iGEM team of the Ludwig-Maximilians-University (LMU) in Munich. The team consists of 10 dedicated members: 7 students and our 3 advisors. Visit our [https://2012.igem.org/Team:LMU-Munich/Team Team] page for more Details.
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[[Team:LMU-Munich/Team|Find out who we are!]]
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[[File:Facebook logo amsterdam.png|70px|left]]
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[[File:Facebook logo amsterdam.png|70px|left|link=https://www.facebook.com/igem.lmu]] Visit us on facebook
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Visit us on facebook
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http://www.facebook.com/igem.lmu
 
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[[File:Exe12_Youtubelogo.png|65px|left]]
 
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Check out our videos!
 
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http://www.youtube.com/user/igemLMUmunich
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[[File:Exe12_Youtubelogo.png|65px|left|link=http://www.youtube.com/user/igemLMUmunich]] Check out our videos!
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Latest revision as of 13:46, 26 October 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

Team-LMU Photo1.jpg

The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

IGEM HQ LMU prize.jpg

[ more news ]

Sporenfreunde

Retrieved from "http://2012.igem.org/Team:LMU-Munich"