Team:BAU-Indonesia/Safety
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+ | <li class="nav5"><a href="https://2012.igem.org/Team:BAU-Indonesia/Modeling">Modeling</a></li> | ||
+ | <li class="nav6"><a href="https://2012.igem.org/Team:BAU-Indonesia/Notebook">Notebook</a></li> | ||
+ | <li class="nav7"><a href="https://2012.igem.org/Team:BAU-Indonesia/Attributions">Attributions</a></li> | ||
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+ | |||
- | + | </div> | |
- | + | ||
+ | </div> <div style="clear: both"></div> | ||
+ | <div id="content" style="background:url(https://static.igem.org/mediawiki/2012/6/67/A.jpg)"> | ||
+ | <!-- | ||
+ | <div class="verticalaccordion"> | ||
+ | <ul> | ||
+ | <li> | ||
+ | <h3>Daftar isi</h3> | ||
+ | <div id="isinya">isi konten di sini bisa juga dengan java script atau yang lainnya</div> | ||
+ | </li> | ||
+ | <li> | ||
+ | <h3>F1</h3> | ||
+ | <div id="isinya">pokonya apa saja isi di sini terserah mau di isi apa</div> | ||
+ | </li> | ||
+ | <li> | ||
+ | <h3>Free Dfloaf</h3> | ||
+ | <div id="isinya">isi apa saja terserah sobat</div> | ||
+ | </li> | ||
+ | <li> | ||
+ | <h3>Free Dosfs</h3> | ||
+ | <div id="isinya">isi konten di sini bisa dengan gambar atau yang lainnya</div> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | --> | ||
+ | <div id="left_column" style="width:auto"> | ||
+ | <!-- Start left news box --> | ||
+ | <div class="left_news_box"> | ||
+ | <div class="left_news_top"></div> | ||
+ | <div class="left_news_bg"> | ||
+ | <h2><b>Safety</b></h2> | ||
+ | <div class="text"> | ||
+ | <h3> | ||
+ | <strong>1. Would any of your project ideas raise safety issues in | ||
+ | |||
+ | terms of: | ||
+ | |||
+ | </strong> | ||
+ | </h3><br> | ||
+ | <h4><strong>Researcher Safety</strong><br></h4> | ||
+ | <h4>We have isolated and cultured the bacteria sample from landfill | ||
+ | |||
+ | at Galuga, West Java Indonesia. To prevent contamination by some | ||
+ | |||
+ | harmfull bacteria, we have followed the instruction of safety | ||
+ | |||
+ | procedure, such as using sensigloves, mask, and washing hand | ||
+ | |||
+ | after sampling. Hazardous chemicals that we used are aclrilamide | ||
+ | |||
+ | and ethidium bromyde. Acrylamide (or acrylic amide) is a simple | ||
+ | |||
+ | organic compound with the chemical formula C3H5NO. It's | ||
+ | |||
+ | potentially hazardous to health (cancer or carcinogenic). | ||
+ | |||
+ | Acrylamide can form long polymer chains known as polyacrylamide, | ||
+ | |||
+ | which is also carcinogenic. Inhalation: irritation, | ||
+ | |||
+ | disorientation, burning, convulsions. Contact with skin: | ||
+ | |||
+ | irritation, disorientation. Eye contact: irritation, a burning | ||
+ | |||
+ | sensation of the eyes, eye damage. | ||
+ | Ethidium bromide will reduce the rate of migration of linear DNA | ||
+ | |||
+ | molecules by 15%. This solution is extremely dangerous and | ||
+ | |||
+ | carcinogenic. All solutions which contained ethidium bromide | ||
+ | |||
+ | should be decontaminated before disposal. Ethidium Bromide | ||
+ | |||
+ | (EtBr) will fluoresce under UV exposure. These dyes can be added | ||
+ | |||
+ | to the gel and buffer so there is no staining after | ||
+ | |||
+ | electrophoresis process. The advantage is more practical, but | ||
+ | |||
+ | the possibility of EtBr contamination is greater. Due to the | ||
+ | |||
+ | carcinogenic factor, it must be avoided for direct contact.<br> </h4> | ||
+ | |||
+ | |||
+ | |||
+ | <h4><strong>Public Safety</strong><br></h4> | ||
+ | <h4>We have ethics to minimize the danger possibility to public. We | ||
+ | |||
+ | usually wear single-used gloves, covered shoes, lab coat, and | ||
+ | |||
+ | mask so the accumulation of materials and impurities are not | ||
+ | |||
+ | exposed to participant directly. We have a liquid discharge | ||
+ | |||
+ | valves which equipped with running water, so it won't settle. We | ||
+ | |||
+ | also have a separated solid waste disposal for potentially | ||
+ | |||
+ | hazardous materials. Those ethics are rules in our work on this | ||
+ | |||
+ | project. </h4> | ||
+ | <br> | ||
+ | |||
+ | <h4><strong>Environmental Safety</strong><br></h4> | ||
+ | <h4> No harmful effect on our enzyme product. E. coli bacteria which | ||
+ | |||
+ | inserted by Cutinase gene has been set for its life period by | ||
+ | |||
+ | sonicator so there'll no blooming act in nature. Destruction of | ||
+ | |||
+ | PET by using our products is done at indoor laboratory so the | ||
+ | |||
+ | waste does not impact to the environment directly. The enzyme | ||
+ | |||
+ | does not cause further effect especially for chemical / physical | ||
+ | |||
+ | danger.No harmful effect on our enzyme product. E. coli bacteria | ||
+ | |||
+ | which inserted by Cutinase gene has been set for its life | ||
+ | |||
+ | period by sonicator so there'll no blooming act in nature. | ||
+ | |||
+ | Destruction of PET by using our products is done at indoor | ||
+ | |||
+ | laboratory so the waste does not impact to the environment | ||
+ | |||
+ | directly. The enzyme does not cause further effect especially | ||
+ | |||
+ | for chemical / physical danger. | ||
+ | </h4> <br> | ||
+ | <h3> <strong>2. Do any of the new BioBrick parts (or devices) that you | ||
+ | |||
+ | made this year raise safety issues? If yes, Did you document these issues in the Registry?</strong> </h3><br> | ||
+ | <h4>No, we didn't document the safety issue to the registry, but our | ||
+ | |||
+ | procedure was according the Standard Operational Procedure of | ||
+ | |||
+ | Molecular Biology Laboratory and Microbiology laboratory in IPB | ||
+ | |||
+ | (Bogor Agricultural University). So we think our project hasn't | ||
+ | |||
+ | any safety risk during we follow the procedure carefully.</h4><br> | ||
+ | |||
+ | |||
+ | <h4><strong>How did you manage to handle the safety issue?</strong></h4><br> | ||
+ | <h4> To handle the security issue of our work, we always consult with | ||
+ | |||
+ | the team advisor and instructor who handle the laboratory | ||
+ | |||
+ | security work in IPB. Our security is guaranteed by following | ||
+ | |||
+ | the standard operating procedures which provided in each | ||
+ | |||
+ | laboratory. As a mechanism to ensure the safety of our work, | ||
+ | |||
+ | first of all we started it by product testing step in the | ||
+ | |||
+ | laboratory before applying it in nature, so when the result is | ||
+ | |||
+ | guaranteed safely, it can be applied in nature.</h4><br> | ||
+ | <h4><strong>How could other teams learn from your experience?</strong></h4><br> | ||
+ | <h4> Other teams can learn from the procedure that we have used. We | ||
+ | |||
+ | use the common procedure that is used by molecular researchers | ||
+ | |||
+ | at the laboratory, so its safety has been tested (as long as we | ||
+ | |||
+ | follow the standard operation procedure had been given). | ||
+ | </h4><br> | ||
+ | |||
+ | |||
+ | <h3><strong>3. Is there a local biosafety group, committee, or review | ||
+ | |||
+ | board at your institution? </strong><h3> <br> | ||
+ | |||
+ | <h4><strong>Which specific biosafety rules or guidelines do you have to | ||
+ | |||
+ | consider in your country?</strong></h4><br> | ||
+ | <h4> We have no local biosafety committee at our institution but | ||
+ | |||
+ | there are Biosafety rules or guidelines in our country. Please | ||
+ | |||
+ | see below :<br> | ||
+ | 1. Regulation Law No. 32 of 2009 about Environmental | ||
+ | |||
+ | Protection & Management<br> | ||
+ | 2. Law No. 7 of 1996 on Food<br> | ||
+ | 3. Law No. 18 of 2002 on National System | ||
+ | |||
+ | Research,Development and Application of Science and | ||
+ | |||
+ | Technology<br> | ||
+ | 4. Law No. 18 of 2004 on Plantation<br> | ||
+ | 5. Law No. 23 of 1992 on Health<br> | ||
+ | 6. Law No. 23 of 1992 on Animal, Fish, and Plant | ||
+ | |||
+ | Quarantine<br> | ||
+ | 7. Law No. 41 of 1999 on Forestry<br> | ||
+ | 8. Government Regulation No. 21 of 2005 on Biosafety of | ||
+ | |||
+ | Genetically Engineered Product<br> | ||
+ | 9. Presidential Decree 39 of 2010 on Biosafety Commision of | ||
+ | |||
+ | Genetically Engineered Product.<br> | ||
+ | 10. INSTITUTIONAL ARRANGEMENT Biosafety Commision | ||
+ | |||
+ | Established by Presidential Decree No. 39/2010 <br> | ||
+ | 11. Biosafety Technical Team In the establishment | ||
+ | |||
+ | process.<br> | ||
+ | 12. Biosafety Clearing House Regarding Article 20 of the | ||
+ | |||
+ | Cartagena Protocol, Indonesia has been establish a Biosafety | ||
+ | |||
+ | Clearing House to facilitate the exchange of scientific, | ||
+ | |||
+ | technical , environmental and legalinformation on LMO. BCH is | ||
+ | |||
+ | coordinated by Ministry of Environment in cooperation with | ||
+ | |||
+ | Indonesia Institute of Science Secretariat of Biosafety | ||
+ | |||
+ | Commission.<br> | ||
+ | 13. Law 32/2009. Every activities may create important risk | ||
+ | |||
+ | on environment, biodiversity and human healthand human safety | ||
+ | |||
+ | have to carry out Environmental Risk Analysis, Risk Assessment, | ||
+ | |||
+ | Risk Management, Risk Communication. | ||
+ | </h4><br> | ||
+ | |||
+ | |||
+ | <h3><strong>4. Do you have any other ideas how to deal with safety | ||
+ | |||
+ | issues that could be useful for future iGEM competitions? How | ||
+ | |||
+ | could parts, devices and systems be made even safer through | ||
+ | |||
+ | biosafety engineering? </strong> | ||
+ | </h3> <br> | ||
+ | <h4>To ensure the biosafety, it will be better to give more | ||
+ | |||
+ | certification letter about the safety of materials which | ||
+ | |||
+ | provided to the participants. Each institution must have their | ||
+ | |||
+ | own fire fighters and laboratory clinic centre. | ||
+ | To minimize the hazardous chemicals we can use the synthetic | ||
+ | |||
+ | biology engineered system. The equipment which is used is fairly | ||
+ | |||
+ | easy to control for these biosafety ethics. The example of | ||
+ | |||
+ | recommended system that has better security is JSA (Job Safety | ||
+ | |||
+ | Analyst).</h4> | ||
+ | |||
+ | |||
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Latest revision as of 22:38, 26 September 2012
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