Team:Tokyo Tech/Projects/Las→Lac assay, Lux→Lac assay/index.htm

From 2012.igem.org

(Difference between revisions)
Line 19: Line 19:
Text
Text
=Conclusion=
=Conclusion=
-
Text
 
-
=Discussion=
 
Text
Text
=Material&Method=
=Material&Method=
Line 27: Line 25:
==Strain==
==Strain==
==Protocol==
==Protocol==
-
1.Prepare overnight culture at 37 degrees for 12hours. (2 tubes for each sample)
+
Text
-
 
+
-
2.Take 30 μl of the overnight culture of reporter cell into LB + antibiotics (Amp + Kan). (→fresh culture)
+
-
 
+
-
3.Incubate the flesh culture of reporter cell] until the observed O.D. reaches around 0.60. and gather the supernatant of culture of inducer cell.
+
-
 
+
-
4.Each sample of reporter cell was divided into 2. Prepare and add AHL mixture to one, cell-synthesized AHL to another one, and DMSO mixture to the other.
+
-
 
+
-
5.Induction of reporter cell for 3 hours.
+
-
 
+
-
6.Fluorometer (FLA5200) and flow cytometry measurements for GFP expression of reporter cell
+

Revision as of 14:49, 4 September 2012

bar

Tokyotechlogo2012.png

Las→Lac assay, Lux→Lac assay

Abstract

We succeeded in constructing and characterizing a NEW Biobrick device (name). This device is a LasR generator We found that the device is (explain)

Introduction

TEXT

Rusult

Text

Conclusion

Text

Material&Method

Construction

Samples

Strain

Protocol

Text
Retrieved from "http://2012.igem.org/Team:Tokyo_Tech/Projects/Las%E2%86%92Lac_assay,_Lux%E2%86%92Lac_assay/index.htm"