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Team:Bielefeld-Germany/Labjournal/week19
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===Monday September 3rd=== | ===Monday September 3rd=== | ||
| - | * '''Team Activity Tests:''' Today our hard-working Team Cultivation offered us some laccases from ''B. pumi''. After fermentation and purification the laccase was still hanging around in imidazole buffer, so we rebuffered it into nanopure water. We incubated the sample with copper and considered to measure the protein concentration before running the activity tests. Using the protocol for determing the protein concentration via Bradford (standards were 1 mg/ml, 1.5 mg/ml and 2 mg/ml BSA) we got a final ''B. pumi'' laccase concentration of 0.22 mg/ml. With this information we designed the following activity test different as usual. As a positive control we took 140 µl of our bought laccase from T. versicolor with a concentration of 0,021 mg/ml. Accordingly to this concentration we applied 14 µl of the ''B. pumi'' laccase to get approximately the same amount of laccase into each well. Additionally we tested 140 µl of the ''B. pumi'' laccase to make sure that we will definitely see any activity. After measuring the samples the whole night we got our results in the morning. It turned out, that our store bought laccase was rapidly active as seen before in our activity tests. The ''B. pumi'' laccase was also active. Using 140 µl of it led to the maximal state of oxidation of ABTS after 45 minutes. With the reduced amount of 14 µl of laccase this optimum was reached after 90 minutes. For our following activity tests we want to include the protein concentration again. | + | * '''Team Activity Tests:''' Today our hard-working Team Cultivation offered us some laccases from ''B. pumi''. After fermentation and purification the laccase was still hanging around in imidazole buffer, so we rebuffered it into nanopure water. We incubated the sample with copper and considered to measure the protein concentration before running the activity tests. Using the protocol for determing the protein concentration via Bradford (standards were 1 mg/ml, 1.5 mg/ml and 2 mg/ml BSA) we got a final ''B. pumi'' laccase concentration of 0.22 mg/ml. With this information we designed the following activity test different as usual. As a positive control we took 140 µl of our bought laccase from ''T. versicolor'' with a concentration of 0,021 mg/ml. Accordingly to this concentration we applied 14 µl of the ''B. pumi'' laccase to get approximately the same amount of laccase into each well. Additionally we tested 140 µl of the ''B. pumi'' laccase to make sure that we will definitely see any activity. After measuring the samples the whole night we got our results in the morning. It turned out, that our store bought laccase was rapidly active as seen before in our activity tests. The ''B. pumi'' laccase was also active. Using 140 µl of it led to the maximal state of oxidation of ABTS after 45 minutes. With the reduced amount of 14 µl of laccase this optimum was reached after 90 minutes. For our following activity tests we want to include the protein concentration again. |
===Tuesday September 4th=== | ===Tuesday September 4th=== | ||
Revision as of 12:40, 4 September 2012
Contents |
Labjournal
Week1 Week2 Week3 Week4 Week5 Week6 Week7 Week8 Week9 Week10 Week11 Week12 Week13 Week14 Week15 Week16 Week17 Week18 Week19
Week 19 (09/03 - 09/09/12)
Monday September 3rd
- Team Activity Tests: Today our hard-working Team Cultivation offered us some laccases from B. pumi. After fermentation and purification the laccase was still hanging around in imidazole buffer, so we rebuffered it into nanopure water. We incubated the sample with copper and considered to measure the protein concentration before running the activity tests. Using the protocol for determing the protein concentration via Bradford (standards were 1 mg/ml, 1.5 mg/ml and 2 mg/ml BSA) we got a final B. pumi laccase concentration of 0.22 mg/ml. With this information we designed the following activity test different as usual. As a positive control we took 140 µl of our bought laccase from T. versicolor with a concentration of 0,021 mg/ml. Accordingly to this concentration we applied 14 µl of the B. pumi laccase to get approximately the same amount of laccase into each well. Additionally we tested 140 µl of the B. pumi laccase to make sure that we will definitely see any activity. After measuring the samples the whole night we got our results in the morning. It turned out, that our store bought laccase was rapidly active as seen before in our activity tests. The B. pumi laccase was also active. Using 140 µl of it led to the maximal state of oxidation of ABTS after 45 minutes. With the reduced amount of 14 µl of laccase this optimum was reached after 90 minutes. For our following activity tests we want to include the protein concentration again.
