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Team: Macquarie Australia/Protocols/SchoolVisit
From 2012.igem.org
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Team members made up more LB media in order to 'draw' on plates with the fluorescent bacteria for Open Day on Saturday. The following protocol was used as previously done in earlier steps. | Team members made up more LB media in order to 'draw' on plates with the fluorescent bacteria for Open Day on Saturday. The following protocol was used as previously done in earlier steps. | ||
| - | + | LB media: (1L total) | |
| - | + | Ingredients: Tryptone 10g, Yeast extract 5g, NaCl 10g, MilliQ water to 1000ml. | |
| - | + | Methods: Dissolved 10g tryptone, 5g yeast extract and 10g NaCl in 800 mL MilliQ water, making use of a magnetic stirrer. Once dissolved, brought volume up to 1L using MilliQ water. Autoclaved 1000ml of the solution (121°C, 15 min, standard liquid cycle). | |
Revision as of 01:47, 4 September 2012
Visit to Green Point Christian College
Today four team members attended Green Point Christian College to present a workshop on Synthetic Biology.
Making more plates for Open Day 8th September
Team members made up more LB media in order to 'draw' on plates with the fluorescent bacteria for Open Day on Saturday. The following protocol was used as previously done in earlier steps.
LB media: (1L total) Ingredients: Tryptone 10g, Yeast extract 5g, NaCl 10g, MilliQ water to 1000ml. Methods: Dissolved 10g tryptone, 5g yeast extract and 10g NaCl in 800 mL MilliQ water, making use of a magnetic stirrer. Once dissolved, brought volume up to 1L using MilliQ water. Autoclaved 1000ml of the solution (121°C, 15 min, standard liquid cycle).
