Team:Amsterdam/maarten/

From 2012.igem.org

(Difference between revisions)
Line 1: Line 1:
<html>
<html>
<head>
<head>
-
<script type="text/javascript" src="jquery-1.2.3.min.js"></script>
 
<script src="//ajax.googleapis.com/ajax/libs/jquery/1.8.0/jquery.min.js"></script>
<script src="//ajax.googleapis.com/ajax/libs/jquery/1.8.0/jquery.min.js"></script>
-
<link rel="stylesheet" type="text/css" href="style.css" />
 
<script type='text/javascript'>
<script type='text/javascript'>
-
 
-
(function() {
 
-
  var ua = navigator.userAgent,
 
-
      iStuff = ua.match(/iPhone/i) || ua.match(/iPad/i),
 
-
      typeOfCanvas = typeof HTMLCanvasElement,
 
-
      nativeCanvasSupport = (typeOfCanvas == 'object' || typeOfCanvas == 'function'),
 
-
      textSupport = nativeCanvasSupport
 
-
        && (typeof document.createElement('canvas').getContext('2d').fillText == 'function');
 
-
  //I'm setting this based on the fact that ExCanvas provides text support for IE
 
-
  //and that as of today iPhone/iPad current text support is lame
 
-
  labelType = (!nativeCanvasSupport || (textSupport && !iStuff))? 'Native' : 'HTML';
 
-
  nativeTextSupport = labelType == 'Native';
 
-
  useGradients = nativeCanvasSupport;
 
-
  animate = !(iStuff || !nativeCanvasSupport);
 
-
})();
 
-
 
-
var Log = {
 
-
  elem: false,
 
-
  write: function(text){
 
-
    if (!this.elem)
 
-
      this.elem = document.getElementById('log');
 
-
    this.elem.innerHTML = text;
 
-
    this.elem.style.left = (500 - this.elem.offsetWidth / 2) + 'px';
 
-
  }
 
-
};
 
-
 
-
function visualization(number){
 
-
$('#infovis').empty()
 
-
json = JSON.parse(unescape($(':input[name=json'+number+']').val()))
 
-
    //init data
 
-
    //init Sunburst
 
-
    var sb = new $jit.Sunburst({
 
-
        //id container for the visualization
 
-
        injectInto: 'infovis',
 
-
        //Distance between levels
 
-
        levelDistance: 90,
 
-
        //Change node and edge styles such as
 
-
        //color, width and dimensions.
 
-
        Node: {
 
-
          overridable: true,
 
-
          type: useGradients? 'gradient-multipie' : 'multipie'
 
-
        },
 
-
        //Select canvas labels
 
-
        //'HTML', 'SVG' and 'Native' are possible options
 
-
        Label: {
 
-
          type: labelType
 
-
        },
 
-
        //Change styles when hovering and clicking nodes
 
-
        NodeStyles: {
 
-
          enable: true,
 
-
          type: 'Native',
 
-
          stylesClick: {
 
-
            'color': '#000000'
 
-
          },
 
-
        },
 
-
        //Add tooltips
 
-
        Tips: {
 
-
          enable: true,
 
-
          onShow: function(tip, node) {
 
-
            var html = "<div class=\"tip-title\">" + node.data.description + "</div>";
 
-
            var data = node.data;
 
-
            if("length" in data) {
 
-
              html += "<b>Length of feature:</b> " + data.length + "bp";
 
-
            }
 
-
if("type" in data){
 
-
html += "<br\><b>Type of feature:</b> " + data.type;
 
-
}
 
-
            tip.innerHTML = html;
 
-
          }
 
-
        },
 
-
        //implement event handlers
 
-
        Events: {
 
-
          enable: true,
 
-
          onClick: function(node) {
 
-
            if(!node) return;
 
-
            //Build detailed information about the file/folder
 
-
            //and place it in the right column.
 
-
            var html = "<h4>" + node.name + "</h4>", data = node.data;
 
-
            if("dna" in data) {
 
-
              html += "<br /><br /><b>Sequence:</b><br /><pre>" + data.dna + "</pre>";
 
-
            }
 
-
            $jit.id('inner-details').innerHTML = html;
 
-
            //hide tip
 
-
            sb.tips.hide();
 
-
            //rotate
 
-
            //sb.rotate(node, animate? 'animate' : 'replot', {
 
-
              //duration: 1000,
 
-
              //transition: $jit.Trans.Quart.easeInOut
 
-
            //});
 
-
          }
 
-
        },
 
-
        // Only used when Label type is 'HTML' or 'SVG'
 
-
        // Add text to the labels.
 
-
        // This method is only triggered on label creation
 
-
        onCreateLabel: function(domElement, node){
 
-
          var labels = sb.config.Label.type,
 
-
              aw = node.getData('length');
 
-
          if (labels === 'HTML' && (node._depth < 2 || aw > 2000)) {
 
-
            domElement.innerHTML = node.name;
 
-
          } else if (labels === 'SVG' && (node._depth < 2 || aw > 2000)) {
 
-
            domElement.firstChild.appendChild(document.createTextNode(node.name));
 
-
          }
 
-
        },
 
-
        // Only used when Label type is 'HTML' or 'SVG'
 
-
        // Change node styles when labels are placed
 
-
        // or moved.
 
-
        onPlaceLabel: function(domElement, node){
 
-
          var labels = sb.config.Label.type;
 
-
          if (labels === 'SVG') {
 
-
            var fch = domElement.firstChild;
 
-
            var style = fch.style;
 
-
            style.display = '';
 
-
            style.cursor = 'pointer';
 
-
            style.fontSize = "0.8em";
 
-
            fch.setAttribute('fill', "#fff");
 
-
          } else if (labels === 'HTML') {
 
-
            var style = domElement.style;
 
-
            style.display = '';
 
-
            style.cursor = 'pointer';
 
-
            style.fontSize = "0.8em";
 
-
            style.color = "#ddd";
 
-
            var left = parseInt(style.left);
 
-
            var w = domElement.offsetWidth;
 
-
            style.left = (left - w / 2) + 'px';
 
-
          }
 
-
        }
 
-
  });
 
-
    //load JSON data.
 
-
    sb.loadJSON(json);
 
-
    //compute positions and plot.
 
-
    sb.refresh();
 
-
 
-
    //end
 
-
}
 
-
 
-
function addForbiddenSequences(){
 
-
forbiddenSequences.addSite('Forbidden-EcoRI','GAATTC')
 
-
forbiddenSequences.addSite('Forbidden-XbaI','GAATTC')
 
-
forbiddenSequences.addSite('Forbidden-SpeI','ACTAGT')
 
-
forbiddenSequences.addSite('Forbidden-PstI','CTGCAG')
 
-
forbiddenSequences.addSite('Forbidden-NotI','GCGGCCGC')
 
-
}
 
-
 
-
var forbiddenSequences = {
 
-
    forbiddenSequencesArray : {},
 
-
addSite : function(name,sequence){
 
-
forbiddenSequences.forbiddenSequencesArray[name] = sequence
 
-
},
 
-
}
 
-
 
-
function checkOccurrence(target,sequence){
 
-
if (sequence.length > target.length){
 
-
        if (sequence.indexOf(target) == -1){
 
-
            return false
 
-
        } else{
 
-
            return true
 
-
        }
 
-
} else{
 
-
if (target.indexOf(sequence) == -1){
 
-
            return false
 
-
        } else{
 
-
            return true
 
-
        }
 
-
}
 
-
}
 
-
 
-
function randomDNA(length){
 
-
chars = 'atgc'
 
-
inForbiddenSite = true
 
-
while (inForbiddenSite === true){
 
-
sequence = ''
 
-
inForbiddenSite = false
 
-
for (k=0; k<length; k++) {
 
-
rnum = Math.floor(Math.random() * chars.length);
 
-
sequence += chars.substring(rnum,rnum+1);
 
-
}
 
-
for (item in forbiddenSequences.forbiddenSequencesArray){
 
-
if (checkOccurrence(sequence,forbiddenSequences.forbiddenSequencesArray[item].toLowerCase()) === false){
 
-
continue
 
-
} else{
 
-
inForbiddenSite = true
 
-
break
 
-
}
 
-
}
 
-
}
 
-
return sequence
 
-
}
 
-
 
-
function getMethyltransferases(){
 
-
    methyltransferases = new Array()
 
-
    sca1 = new Object()
 
-
    sca1.target = "AGTACT"
 
-
    sca1.sequence = "tccgggcgggactttggatatgtgatacagtcgtccgctgcactatggaatcgactctctacattctcacagagaggaaaagccttggacaccaggcttgcagacatcaagaaggccctggggaagccgtactacgaaacctcggatgtccttctttaccacggcgacagtcttgagctgctcaagtcaatgcctcagcagattttcgaccttaccgtaactagcccaccttacaatattggcaaagagtacgagggtgtactgtcgatcgaggaatacatttcctggtgcgagacatggatgtcgcgcgttcatagggcgaccagcgcaggcggcgcattttggctcaatgttgggtacgtccctgtcccgaaccaaggaaaagcagtcccgattccttacctcttgtgggacaagagtccgttctacatgatccaggaagttgtctggaattacggggcgggagtggcgtctcgaaaatcgttttccccgcgcaatgaaaagtttctctggtatgtgcgcgacccgctgaattattacttcgacctcgattcggtgcgcgacccaaatgtgaaataccccaaccagaaaaagaatgggaagctcaaatgcaacccgttggggaaaaatcccactgacgtttggcagttccccaaggttacgtcgggcgcgaagagatcaagcgtggagcgcaccgcccatccggcacaattcccgtctgctgtcattgaacgggtcatcaaggcgtgcagcccttccgacggcgtcatcctggacccattcctcggttccggaacgacctcgctgaccgccagaaagcaaggccggtgcagcgtcggtatcgaaatccgcgaagactacctcgacatcgcggtgggacgcctggaggcggaggcgcaatccctcttctag"
 
-
    sca1.description = "M.Sca1"
 
-
sca2 = new Object()
 
-
sca2.target = "AGTACC"
 
-
sca2.sequence = "tccgggcgggactttggatatgtgatacagtcgtccgctgcactatggaatcgactctctacattctcacagagaggaaaagccttggacaccaggcttgcagacatcaagaaggccctggggaagccgtactacgaaacctcggatgtccttctttaccacggcgacagtcttgagctgctcaagtcaatgcctcagcagattttcgaccttaccgtaactagcccaccttacaatattggcaaagagtacgagggtgtactgtcgatcgaggaatacatttcctggtgcgagacatggatgtcgcgcgttcatagggcgaccagcgcaggcggcgcattttggctcaatgttgggtacgtccctgtcccgaaccaaggaaaagcagtcccgattccttacctcttgtgggacaagagtccgttctacatgatccaggaagttgtctggaattacggggcgggagtggcgtctcgaaaatcgttttccccgcgcaatgaaaagtttctctggtatgtgcgcgacccgctgaattattacttcgacctcgattcggtgcgcgacccaaatgtgaaataccccaaccagaaaaagaatgggaagctcaaatgcaacccgttggggaaaaatcccactgacgtttggcagttccccaaggttacgtcgggcgcgaagagatcaagcgtggagcgcaccgcccatccggcacaattcccgtctgctgtcattgaacgggtcatcaaggcgtgcagcccttccgacggcgtcatcctggacccattcctcggttccggaacgacctcgctgaccgccagaaagcaaggccggtgcagcgtcggtatcgaaatccgcgaagactacctcgacatcgcggtgggacgcctggaggcggaggcgcaatccctcttctagg"
 
-
    sca2.description = "M.Sca2"
 
-
methyltransferases[0] = sca1
 
-
methyltransferases[1] = sca2
 
-
addForbiddenSequence(methyltransferases,'hasTarget')
 
-
    return methyltransferases
 
-
}
 
-
 
-
function getZincFingers(){
 
-
    zincFingers = new Array()
 
-
    rawData = document.plasmidForm.zf.value
 
-
    rawData = rawData.split('>')
 
-
    rawData.reverse().pop()
 
-
    rawData.reverse()
 
-
    for (zf in rawData){
 
-
        zfFormatted = rawData[zf].split('\n')
 
-
        zfDescription = zfFormatted[0]
 
-
        zfTarget = zfDescription.split('|')[5]
 
-
        zfSequence = ''
 
-
        for (i=1;i<zfFormatted.length;i++){
 
-
            zfSequence+=zfFormatted[i]
 
-
        }
 
-
        zfObject = new Object()
 
-
        zfObject.target = zfTarget
 
-
        zfObject.sequence = zfSequence
 
-
        zfObject.description = zfDescription
 
-
        zincFingers[zf] = zfObject
 
-
    }
 
-
    addForbiddenSequence(zincFingers,'hasTarget')
 
-
    return zincFingers
 
-
}
 
-
 
-
function getLinkers(){
 
-
    linkers = new Array()
 
-
    linker1 = new Object()
 
-
    linker1.sequence = "gcacccggc"
 
-
    linker1.description = "Linker 1"
 
-
linker2 = new Object()
 
-
linker2.sequence = "gcaccccgccc"
 
-
linker2.description = "Linker 2"
 
-
    linkers[0] = linker1
 
-
linkers[1] = linker2
 
-
addForbiddenSequence(linkers,'noTarget')
 
-
    return linkers
 
-
}
 
-
 
-
function getMycTags(){
 
-
    mycTags = new Array()
 
-
    mycTag1 = new Object()
 
-
    mycTag1.sequence = "gaacagaaactcatctctgaagaggatctg"
 
-
    mycTag1.description = "Myc Tag 1"
 
-
mycTag2 = new Object()
 
-
mycTag2.sequence = "gaacagaaactcatctctgaagagaatctgaagactgagtcagt"
 
-
mycTag2.description = "Myc Tag 2"
 
-
    mycTags[0] = mycTag1
 
-
mycTags[1] = mycTag2
 
-
addForbiddenSequence(mycTags,'noTarget')
 
-
    return mycTags
 
-
}
 
-
 
-
function getTerminators(){
 
-
    terminators = new Array()
 
-
    terminator1 = new Object()
 
-
    terminator1.sequence = "tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt"
 
-
    terminator1.description = "B0014 containing BOO12 and BOO11 terminators"
 
-
terminator2 = new Object()
 
-
terminator2.sequence = "tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattatttt"
 
-
    terminator2.description = "Terminator 2"
 
-
terminators[0] = terminator1
 
-
terminators[1] = terminator2
 
-
addForbiddenSequence(terminators,'noTarget')
 
-
    return terminators
 
-
}
 
-
 
-
function getRBS(){
 
-
    rbs = new Array()
 
-
    rbs1 = new Object()
 
-
    rbs1.sequence = "tcacacaggaaag"
 
-
    rbs1.description = "BBa_B0032"
 
-
    rbs2 = new Object()
 
-
    rbs2.sequence = "aaaaaaaaaaaccccaa"
 
-
    rbs2.description = "RBS2"
 
-
    rbs[0] = rbs1
 
-
    rbs[1] = rbs2
 
-
addForbiddenSequence(rbs,'noTarget')
 
-
    return rbs
 
-
}
 
-
 
-
function addForbiddenSequence(array,type){
 
-
if (type == 'hasTarget'){
 
-
for (item in array){
 
-
forbiddenSequences.addSite(array[item].description,array[item].target)
 
-
}
 
-
} else{
 
-
for (item in array){
 
-
forbiddenSequences.addSite(array[item].description,array[item].sequence)
 
-
}
 
-
}
 
-
 
-
}
 
-
 
-
function getDate(){
 
-
    today = new Date()
 
-
    dd = today.getDate()
 
-
    mm = today.getMonth()+1
 
-
    yyyy = today.getFullYear()
 
-
    if(dd<10){
 
-
        dd='0'+dd
 
-
    }
 
-
    if(mm<10){
 
-
        mm='0'+mm
 
-
    }
 
-
    today = dd+'-'+mm.substring(0,2)+'-'+yyyy;
 
-
    return today
 
-
}
 
-
 
-
function generateWhiteSpace(amount){
 
-
        output = ''
 
-
        for (i=0;i<amount;i++){
 
-
            output += '&nbsp;'
 
-
        }
 
-
        return output
 
-
}
 
-
 
-
function staticGenbank(name){
 
-
output = 'LOCUS'
 
-
        output += generateWhiteSpace(16)
 
-
        output += name
 
-
        output += generateWhiteSpace(8)
 
-
        output += memoryPlasmid.dna.length+' bp DNA '
 
-
        output += generateWhiteSpace(3)
 
-
        output += 'linear'
 
-
        output += generateWhiteSpace(7)
 
-
        output += getDate()+'<br>'
 
-
        output += 'DEFINITION<br>'
 
-
        output += 'ACCESSION<br>'
 
-
        output += 'VERSION<br>'
 
-
        output += 'SOURCE<br>'
 
-
        output += ' &nbsp;ORGANISM<br>'
 
-
        output += 'COMMENT<br>'
 
-
        output += 'FEATURES'
 
-
        output += generateWhiteSpace(13)
 
-
        output += 'Location/Qualifiers<br>'
 
-
return output
 
-
}
 
-
 
-
function genbankRestriction(targetSites){
 
-
restrictionPlasmidOutput = staticGenbank('restriction_plasmid')
 
-
restrictionOrigin = memoryPlasmid.features[1].sequence
 
-
for (key in targetSites){
 
-
    restrictionOrigin += randomDNA(10)
 
-
    restrictionPlasmidOutput += generateWhiteSpace(5)
 
-
restrictionPlasmidOutput += 'misc_feature'
 
-
restrictionPlasmidOutput += generateWhiteSpace(5)
 
-
restrictionPlasmidOutput += restrictionOrigin.length+1+'...'
 
-
    restrictionOrigin += targetSites[key].zincFinger
 
-
restrictionPlasmidOutput += restrictionOrigin.length+'<br>'
 
-
restrictionOrigin += randomDNA(10)
 
-
restrictionPlasmidOutput += generateWhiteSpace(5)
 
-
restrictionPlasmidOutput += 'misc_feature'
 
-
restrictionPlasmidOutput += generateWhiteSpace(5)
 
-
restrictionPlasmidOutput += restrictionOrigin.length+1+'...'
 
-
    restrictionOrigin += targetSites[key].methyltransferase
 
-
restrictionPlasmidOutput += restrictionOrigin.length+'<br>'
 
-
restrictionOrigin += targetSites[key].methyltransferase
 
-
}
 
-
restrictionOrigin = restrictionOrigin.toLowerCase()
 
-
        restrictionOrigin = restrictionOrigin.replace(/[atgc]{10}/g,'$& ').replace(/amp;/g,'').split(' ')
 
-
        pos = 1
 
-
        newString = ''
 
-
        restrictionPlasmidOutput += 'ORIGIN<br>'
 
-
        for (i=0;i<restrictionOrigin.length;i++){
 
-
            newString = newString+' '+restrictionOrigin[i]
 
-
            if ((i+1)%6===0){
 
-
                whiteSpace = ''
 
-
                for (j=0;j<9-pos.toString().length;j++){
 
-
                    whiteSpace+= '&nbsp;'
 
-
                }
 
-
                newString = whiteSpace+pos + ' ' + newString
 
-
                restrictionPlasmidOutput+=newString+'<br>'
 
-
                newString = ''
 
-
            } else if ((i+1)%6==1){
 
-
                  if (i!=0){
 
-
                      pos+=60
 
-
                  }
 
-
              }
 
-
        }
 
-
        if (restrictionOrigin.length%6 != 0){
 
-
            newString = ''
 
-
            whiteSpace = ''
 
-
            for (j=0;j<9-pos.toString().length;j++){
 
-
                whiteSpace+= '&nbsp;'
 
-
            }
 
-
            newString+=whiteSpace+pos
 
-
            for (i=0;i<(restrictionOrigin.length%6);i++){
 
-
                newString+=' '+restrictionOrigin[restrictionOrigin.length-restrictionOrigin.length%6+i]
 
-
            }
 
-
            restrictionPlasmidOutput+=newString+'<br>'
 
-
        }
 
-
restrictionSiteWindow = window.open()
 
-
restrictionSiteWindow.document.write(restrictionPlasmidOutput)
 
-
restrictionSiteWindow.focus()
 
-
}
 
-
function genbankMemory(){
 
-
        memoryPlasmidOutput = staticGenbank('memory_plasmid')
 
-
        for (item in memoryPlasmid.memoryObjects){
 
-
    for (key in memoryPlasmid.memoryObjects[item]){
 
-
memoryPlasmidOutput += generateWhiteSpace(5)
 
-
memoryPlasmidOutput += 'misc_feature'
 
-
memoryPlasmidOutput += generateWhiteSpace(5)
 
-
memoryPlasmidOutput += memoryPlasmid.memoryObjects[item][key].start+'...'+memoryPlasmid.memoryObjects[item][key].end+'<br>'
 
-
memoryPlasmidOutput += generateWhiteSpace(10)
 
-
memoryPlasmidOutput += '/label='+memoryPlasmid.memoryObjects[item][key].description+'<br>'
 
-
}
 
-
           
 
-
        }
 
-
        origin = memoryPlasmid.dna
 
-
        origin = origin.replace(/[atgc]{10}/g,'$& ').replace(/amp;/g,'').split(' ')
 
-
        pos = 1
 
-
        newString = ''
 
-
        memoryPlasmidOutput += 'ORIGIN<br>'
 
-
        for (i=0;i<origin.length;i++){
 
-
            newString = newString+' '+origin[i]
 
-
            if ((i+1)%6===0){
 
-
                whiteSpace = ''
 
-
                for (j=0;j<9-pos.toString().length;j++){
 
-
                    whiteSpace+= '&nbsp;'
 
-
                }
 
-
                newString = whiteSpace+pos + ' ' + newString
 
-
                memoryPlasmidOutput+=newString+'<br>'
 
-
                newString = ''
 
-
            } else if ((i+1)%6==1){
 
-
                  if (i!=0){
 
-
                      pos+=60
 
-
                  }
 
-
              }
 
-
        }
 
-
        if (origin.length%6 != 0){
 
-
            newString = ''
 
-
            whiteSpace = ''
 
-
            for (j=0;j<9-pos.toString().length;j++){
 
-
                whiteSpace+= '&nbsp;'
 
-
            }
 
-
            newString+=whiteSpace+pos
 
-
            for (i=0;i<(origin.length%6);i++){
 
-
                newString+=' '+origin[origin.length-origin.length%6+i]
 
-
            }
 
-
            memoryPlasmidOutput+=newString+'<br>'
 
-
        }
 
-
 
-
        myWindow=window.open()
 
-
        myWindow.document.write(memoryPlasmidOutput)
 
-
        myWindow.focus()
 
-
 
-
}
 
-
 
-
function formatPlasmidInput(backbone){
 
-
        backbone = backbone.split('\n')
 
-
        originBoolean = false
 
-
        backboneName = ''
 
-
        sequence = ''
 
-
        if (backbone[0].toLowerCase().indexOf('locus') != -1){
 
-
            for (i=0;i<backbone.length;i++){
 
-
                if (originBoolean === true){
 
-
                    if (backbone[i].indexOf('//') == -1){
 
-
                      appendThis = backbone[i].replace(/[0-9]|\s|\n|\t/g,'')
 
-
                      sequence = sequence+appendThis
 
-
                    } else{
 
-
                        originalBoolean = false
 
-
                    }
 
-
                }
 
-
                if (backbone[i].indexOf('locus') != -1){
 
-
                    backboneName = backbone[i].split(/\s+|\t+/g)[1]
 
-
                }
 
-
                if (backbone[i].indexOf('origin') != -1){
 
-
                    originBoolean = true
 
-
                }
 
-
            }
 
-
        } else if (backbone[0][0] == '>'){
 
-
                for (i=0;i<backbone.length;i++){
 
-
                    if (backbone[i][0] != '>'){
 
-
                        appendThis = backbone[i].replace(/[0-9]|\s|\n|\t/g,'')
 
-
                        sequence = sequence+appendThis
 
-
                    } else{
 
-
                        backboneName = backbone[i].replace('>','')
 
-
                    }
 
-
                }
 
-
        }
 
-
        memoryPlasmid.addFeature(1,sequence.length,'Backbone',sequence,backboneName,'')
 
-
        return sequence
 
-
}
 
-
 
-
function feature(start,end,type,sequence,description,featureObject){
 
-
this.start = start;
 
-
this.end = end;
 
-
this.type = type;
 
-
    this.sequence = sequence;
 
-
    this.description = description;
 
-
this.featureObject = featureObject
 
-
}
 
-
 
-
var restrictionPlasmid = {
 
-
backboneName : '',
 
-
dna : '',
 
-
features : {},
 
-
    setBackboneName : function(backboneName){
 
-
        restrictionlasmid.backboneName = backboneName
 
-
    },
 
-
setDNA : function(dna){
 
-
restrictionPlasmid.dna = dna
 
-
},
 
-
addFeature : function(start,end,type,sequence,description){
 
-
restrictionPlasmid.features[start] = new feature(start,end,type,sequence,description)
 
-
},
 
-
addRecognitionAreaObject : function(zfTarget,mtaseTarget){
 
-
 
-
recognitionPlasmid.zfTarget = zfTarget
 
-
recognitionPlasmid.mtaseTarget = mtaseTarget
 
-
}
 
-
}
 
-
 
-
var memoryPlasmid = {
 
-
    backboneName : '',
 
-
dna : '',
 
-
features : {},
 
-
memoryObjects : {},
 
-
    setBackboneName : function(backboneName){
 
-
        memoryPlasmid.backboneName = backboneName
 
-
    },
 
-
setDNA : function(dna){
 
-
memoryPlasmid.dna = dna
 
-
},
 
-
addMemoryObject : function(sensor,rbs,zincFinger,linker,mycTag,methyltransferase,terminator){
 
-
memoryObject = new Object()
 
-
memoryObject.sensor = sensor
 
-
memoryObject.rbs = rbs
 
-
memoryObject.zincFinger = zincFinger
 
-
memoryObject.linker = linker
 
-
memoryObject.mycTag = mycTag
 
-
memoryObject.methyltransferase = methyltransferase
 
-
memoryObject.terminator = terminator
 
-
    memoryPlasmid.memoryObjects[sensor.start] = memoryObject
 
-
},
 
-
addFeature : function(start,end,type,sequence,description){
 
-
memoryPlasmid.features[start] = new feature(start,end,type,sequence,description)
 
-
}
 
-
}
 
-
 
-
function sensorObject(name,sequence){
 
-
        this.name = name
 
-
        this.sequence = sequence
 
-
 
-
}
 
-
     
 
-
function formatSensors(sensors){
 
-
        sensorArray = new Array()
 
-
        if (sensors.indexOf('LOCUS') != -1){
 
-
            sensors = sensors.split('LOCUS')
 
-
            sensors.reverse().pop()
 
-
            for (i=0;i<sensors.length;i++){
 
-
                originBoolean = false
 
-
                sensorName = ''
 
-
                sequence = ''
 
-
                sensor = sensors[i].split('\n')
 
-
                for (j=0;j<sensor.length;j++){
 
-
                    if (j===0){
 
-
                        sensorName = sensor[j]
 
-
                    }
 
-
                    if (originBoolean === true){
 
-
                        if (sensor[j].indexOf('//') == -1){
 
-
                            appendThis = sensor[j].replace(/[0-9]|\s|\n|\t/g,'')
 
-
                            sequence += appendThis
 
-
                        } else {
 
-
                            originBoolean = false
 
-
                        }
 
-
                    }
 
-
                    if (sensor[j].indexOf('ORIGIN') != -1){
 
-
                        originBoolean = true
 
-
                    }
 
-
                }
 
-
                sensorArray[i] = new sensorObject(sensorName,sequence)
 
-
            }
 
-
        } else if (sensors[0] == '>'){
 
-
            sensors = sensors.split('//')
 
-
            sensors.pop()
 
-
            sensorName = ''
 
-
            for (i=0;i<sensors.length;i++){
 
-
                sensor = sensors[i].split('\n')
 
-
                sequence = ''
 
-
                for (j=0;j<sensor.length;j++){
 
-
                    if (sensor[j][0] != '>'){
 
-
                        appendThis = sensor[j].replace(/[0-9]|\s|\n|\t/g,'')
 
-
                        sequence = sequence+appendThis
 
-
                    } else if (sensor[j][0] == '>'){
 
-
                        sensorName = sensor[j].replace('>','')
 
-
                    } 
 
-
                }
 
-
                sensorArray[i] = new sensorObject(sensorName,sequence)
 
-
            }
 
-
        }
 
-
        return sensorArray
 
-
}
 
-
 
-
function formatPartsregistryPromoters(){
 
-
        promoters = new Array()
 
-
        $("input:checkbox[name=promoter]:checked").each(function(){
 
-
            promoter = $(this).val().split(':')
 
-
            promoters.push(new sensorObject(promoter[0],promoter[2]))
 
-
        })
 
-
        return promoters
 
-
}
 
-
 
-
function configuratePlasmid(sensorArray,number){
 
-
memoryPlasmid.features = {}
 
-
memoryPlasmid.memoryObjects = {}
 
-
        backbone = document.plasmidForm.plasmid.value.toLowerCase()
 
-
        methyltransferases = getMethyltransferases()
 
-
        zincFingers = getZincFingers()
 
-
        linkers = getLinkers()
 
-
        mycTags = getMycTags()
 
-
        rbs = getRBS()
 
-
        terminators = getTerminators()
 
-
        plasmidSequence = formatPlasmidInput(backbone)
 
-
        memoryPlasmid.setDNA(plasmidSequence)
 
-
        for (i=0;i<sensorArray.length;i++){
 
-
    featureArray = new Array()
 
-
featureArray[0] = new feature(memoryPlasmid.dna.length+1,memoryPlasmid.dna.length+sensorArray[i].sequence.length,'Sensor',sensorArray[i].sequence,sensorArray[i].name,sensorArray[i])
 
-
            memoryPlasmid.setDNA(memoryPlasmid.dna+sensorArray[i].sequence)
 
-
            j = Math.floor((Math.random()*rbs.length))
 
-
            featureArray[1] = new feature(memoryPlasmid.dna.length+1,memoryPlasmid.dna.length+rbs[j].sequence.length,'RBS',rbs[j].sequence,rbs[j].description,rbs[j])
 
-
            memoryPlasmid.setDNA(memoryPlasmid.dna+rbs[j].sequence+randomDNA(3))
 
-
            memoryPlasmid.setDNA(memoryPlasmid.dna+'atg'+randomDNA(10))
 
-
            for (j=0;j<zincFingers.length;j++){
 
-
                if (checkOccurrence(zincFingers[j].target.toLowerCase(),memoryPlasmid.dna) === true || checkOccurrence(zincFingers[j].sequence.toLowerCase(),memoryPlasmid.dna) === true){
 
-
                    continue
 
-
                } else{
 
-
                    featureArray[2] = new feature(memoryPlasmid.dna.length+1,memoryPlasmid.dna.length+zincFingers[j].sequence.length,'Zinc Finger',zincFingers[j].sequence,'Zinc Finger '+zincFingers[j].description.split('|')[0],zincFingers[j])
 
-
                    memoryPlasmid.setDNA(memoryPlasmid.dna+zincFingers[j].sequence)
 
-
                    break
 
-
                }
 
-
            }
 
-
            j = Math.floor((Math.random()*linkers.length))
 
-
            featureArray[3] = new feature(memoryPlasmid.dna.length+1,memoryPlasmid.dna.length+linkers[j].sequence.length,'Linker',linkers[j].sequence,linkers[j].description,linkers[j])
 
-
    memoryPlasmid.setDNA(memoryPlasmid.dna+linkers[j].sequence)
 
-
            j = Math.floor((Math.random()*mycTags.length))
 
-
            featureArray[4] = new feature(memoryPlasmid.dna.length+1,memoryPlasmid.dna.length+mycTags[j].sequence.length,'Myc Tag',mycTags[j].sequence,mycTags[j].description,mycTags[j])
 
-
    memoryPlasmid.setDNA(memoryPlasmid.dna+mycTags[j].sequence)
 
-
            j = Math.floor((Math.random()*methyltransferases.length))
 
-
    featureArray[5] = new feature(memoryPlasmid.dna.length+1,memoryPlasmid.dna.length+methyltransferases[j].sequence.length,'Methyltransferase',methyltransferases[j].sequence,methyltransferases[j].description,methyltransferases[j])
 
-
            memoryPlasmid.setDNA(memoryPlasmid.dna+methyltransferases[j].sequence)
 
-
            j = Math.floor((Math.random()*terminators.length))
 
-
            featureArray[6] = new feature(memoryPlasmid.dna.length+1,memoryPlasmid.dna.length+terminators[j].sequence.length,'Terminator',terminators[j].sequence,terminators[j].description,terminators[j])
 
-
    memoryPlasmid.setDNA(memoryPlasmid.dna+terminators[j].sequence+randomDNA(10))
 
-
            memoryPlasmid.addMemoryObject(featureArray[0],featureArray[1],featureArray[2],featureArray[3],featureArray[4],featureArray[5],featureArray[6])
 
-
}
 
-
restrictionPlasmid.setDNA(memoryPlasmid.features[1].sequence)
 
-
for (item in memoryPlasmid.memoryObjects){
 
-
featureArray = new Array()
 
-
mtaseStart = restrictionPlasmid.dna.length+1
 
-
mtaseEnd = restrictionPlasmid.dna.length+memoryPlasmid.memoryObjects[item].methyltransferase.featureObject.target.length
 
-
mtaseType = 'Restriction Site'
 
-
mtaseTarget = memoryPlasmid.memoryObjects[item].methyltransferase.featureObject.target.toLowerCase()
 
-
mtaseDescription = memoryPlasmid.memoryObjects[item].methyltransferase.featureObject.description+' restriction site'
 
-
featureArray[0] = new feature(mtaseStart,mtaseEnd,mtaseType,mtaseTarget,mtaseDescription)
 
-
restrictionPlasmid.setDNA(restrictionPlasmid.dna+mtaseTarget)
 
-
zfStart = restrictionPlasmid.dna.length+1
 
-
zfEnd = restrictionPlasmid.dna.length+memoryPlasmid.memoryObjects[item].zincFinger.featureObject.target.length
 
-
zfType = 'Restriction Site'
 
-
zfTarget = memoryPlasmid.memoryObjects[item].zincFinger.featureObject.target
 
-
zfDescription = memoryPlasmid.memoryObjects[item].zincFinger.featureObject.description+' restriction site'
 
-
featureArray[1] = new feature(zfStart,zfEnd,zfType,zfTarget,zfDescription)
 
-
restrictionPlasmid.setDNA(restrictionPlasmid.dna+mtaseTarget)
 
-
restrictionPlasmid.mtaseTarget = featureArray[0]
 
-
restrictionPlasmid.zfTarget = featureArray[1]
 
-
}
 
-
json = createJSONMemory(number)
 
-
//genbankMemory()
 
-
$('#visualizationButtons').append('<input type="hidden" name="json'+number+'" value="'+escape(JSON.stringify(json))+'"/><input class="submit" type="submit" value="Memory Plasmid '+(number+1)+'" onclick="visualization('+number+');return false"/>');
 
-
}
 
function setup(){
function setup(){
-
$('#visualizationButtons').empty()
+
    document.write('bla')
-
$('#infovis').empty()
+
-
$('#readout').empty()
+
-
addForbiddenSequences()
+
-
sensors = document.plasmidForm.sensors.value.toLowerCase()
+
-
sensorArray = formatSensors(sensors)
+
-
        promoters = formatPartsregistryPromoters()
+
-
        sensorArray = promoters.concat(sensorArray)
+
-
tempArray = new Array()
+
-
j = 0
+
-
number = 0
+
-
targetSites = new Array()
+
-
for (sensor in sensorArray){
+
-
tempArray[j] = sensorArray[sensor]
+
-
j++
+
-
if (j%5 === 0 && j !== 0){
+
-
configuratePlasmid(tempArray,number)
+
-
number++
+
-
tempArray = new Array()
+
-
j = 0
+
-
for (item in memoryPlasmid.memoryObjects){
+
-
site = new Object()
+
-
site.zincFingerDescription = memoryPlasmid.memoryObjects[item].zincFinger.featureObject.description
+
-
site.zincFinger = memoryPlasmid.memoryObjects[item].zincFinger.featureObject.target
+
-
site.zincFingerLength = site.zincFinger.length
+
-
site.methyltransferaseDescription = memoryPlasmid.memoryObjects[item].methyltransferase.featureObject.description
+
-
site.methyltransferase = memoryPlasmid.memoryObjects[item].methyltransferase.featureObject.target
+
-
site.methyltransferaseLength = site.methyltransferase.length
+
-
site.sensor = memoryPlasmid.memoryObjects[item].sensor.description
+
-
targetSites.push(site)
+
-
}
+
-
}
+
-
}
+
-
if (j%5 !== 0){
+
-
configuratePlasmid(tempArray,number)
+
-
for (item in memoryPlasmid.memoryObjects){
+
-
site = new Object()
+
-
site.zincFingerDescription = memoryPlasmid.memoryObjects[item].zincFinger.featureObject.description
+
-
site.zincFinger = memoryPlasmid.memoryObjects[item].zincFinger.featureObject.target
+
-
site.zincFingerLength = site.zincFinger.length
+
-
site.methyltransferaseDescription = memoryPlasmid.memoryObjects[item].methyltransferase.featureObject.description
+
-
site.methyltransferase = memoryPlasmid.memoryObjects[item].methyltransferase.featureObject.target
+
-
site.methyltransferaseLength = site.methyltransferase.length
+
-
site.sensor = memoryPlasmid.memoryObjects[item].sensor.description
+
-
targetSites.push(site)
+
-
}
+
-
}
+
-
//genbankRestriction(targetSites)
+
-
json = createJSONRestriction(targetSites)
+
-
$('#visualizationButtons').append('<input type="hidden" name="json-1" value="'+escape(JSON.stringify(json))+'"/><input class="submit" type="submit" value="Restriction Plasmid" onclick="visualization(-1);return false"/>');
+
-
$('#readout').append('<input type="textfield" name="readout" value=""/><input class="submit" type="submit" value="Submit" onClick="getReadout();return false"/>');
+
}
}
-
 
-
function getReadout(){
 
-
inputArray = new Array()
 
-
json = JSON.parse(unescape($(':input[name=json-1]').val()))
 
-
inputs = $(':input[name=readout]').val()
 
-
inputArray = inputs.split(',')
 
-
totalLength = json.children[0].data.length
 
-
uncutArray = new Array()
 
-
i = 0
 
-
for (child in json.children){
 
-
totalLength+=json.children[child].data.length
 
-
if (json.children[child].data.description.indexOf('Methyltransferase') != -1){
 
-
uncutArray[i] = [json.children[child].id,totalLength]
 
-
i++
 
-
}
 
-
}
 
-
bandsArray = new Array ()
 
-
j = 0
 
-
for (item in uncutArray){
 
-
i = 0
 
-
tempBandArray = new Array()
 
-
tempBandArray[i] = [uncutArray[item][1],uncutArray[item][0]]
 
-
i++
 
-
for (bandArray in bandsArray){
 
-
for (band in bandsArray[bandArray]){
 
-
tempBandArray[i] = [uncutArray[item][1] - bandsArray[bandArray][band][0],uncutArray[item][0]]
 
-
i++
 
-
}
 
-
}
 
-
bandsArray[j] = tempBandArray
 
-
j++
 
-
}
 
-
matchingBands = new Array()
 
-
i = 0
 
-
for (input in inputArray){
 
-
difference = 100000000000
 
-
bandSize = inputArray[input]
 
-
for (bandArray in bandsArray){
 
-
for (band in bandsArray[bandArray]){
 
-
if (bandSize > bandsArray[bandArray][band][0]){
 
-
tempDifference = bandSize - bandsArray[bandArray][band][0]
 
-
} else{
 
-
tempDifference = bandsArray[bandArray][band][0] - bandSize
 
-
}
 
-
if (tempDifference < difference){
 
-
matchingBand = bandsArray[bandArray][band][1]
 
-
difference = tempDifference
 
-
}
 
-
}
 
-
}
 
-
matchingBands[i] = matchingBand
 
-
i++
 
-
}
 
-
i = 0
 
-
sensedSignals = new Array()
 
-
for (matchingBand in matchingBands){
 
-
for (child in json.children){
 
-
if (json.children[child].id == matchingBands[matchingBand]){
 
-
sensedSignals[i] = json.children[child].data.sensor
 
-
i++
 
-
}
 
-
}
 
-
}
 
-
for (signal in sensedSignals){
 
-
$('#readout').append('<br>'+sensedSignals[signal])
 
-
}
 
-
}
 
-
 
-
function createJSONRestriction(targetSites){
 
-
json = {
 
-
      "children": [],
 
-
      "data": {
 
-
        "$type": "none",
 
-
      },
 
-
      "id": "Source",
 
-
      "name": "Restriction Plasmid"
 
-
    };
 
-
 
-
json.children[0] = {}
 
-
json.children[0].data = {}
 
-
json.children[0].data.description = memoryPlasmid.features[1].description
 
-
json.children[0].data.$color = '#B2ABF4'
 
-
json.children[0].data.length = memoryPlasmid.features[1].end - memoryPlasmid.features[1].start
 
-
json.children[0].data.$angularWidth = 1000
 
-
json.children[0].data.type = memoryPlasmid.features[1].type
 
-
json.children[0].data.dna = memoryPlasmid.dna
 
-
json.children[0].children = []
 
-
json.children[0].name = memoryPlasmid.features[1].description
 
-
json.children[0].id = memoryPlasmid.features[1].description
 
-
totalLength = memoryPlasmid.dna.length
 
-
i = 1
 
-
for (item in targetSites){
 
-
site = targetSites[item]
 
-
json.children[i] = {}
 
-
json.children[i].data = {}
 
-
json.children[i].children = []
 
-
json.children[i].data.description = '120bp random DNA'
 
-
json.children[i].data.$color = '#FFFF00'
 
-
json.children[i].data.length = 120
 
-
json.children[i].data.$angularWidth = 1000
 
-
json.children[i].data.dna = randomDNA(120+i*10)
 
-
json.children[i].name = '120bp DNA'
 
-
json.children[i].id = i
 
-
i++
 
-
json.children[i] = {}
 
-
json.children[i].data = {}
 
-
json.children[i].children = []
 
-
json.children[i].data.description = site.zincFingerDescription
 
-
json.children[i].data.$color = '#FF0000'
 
-
json.children[i].data.length = site.zincFingerLength
 
-
json.children[i].data.$angularWidth = 1000
 
-
json.children[i].data.dna = site.zincFinger
 
-
json.children[i].name = site.zincFingerDescription.split('|')[0]+'|'+site.zincFingerDescription.split('|')[1]
 
-
json.children[i].id = i
 
-
i++
 
-
json.children[i] = {}
 
-
json.children[i].data = {}
 
-
json.children[i].children = []
 
-
json.children[i].data.description = '120bp random DNA'
 
-
json.children[i].data.$color = '#FFFF00'
 
-
json.children[i].data.length = 120
 
-
json.children[i].data.$angularWidth = 1000
 
-
json.children[i].data.dna = randomDNA(120)
 
-
json.children[i].name = '120bp DNA'
 
-
json.children[i].id = i
 
-
i++
 
-
json.children[i] = {}
 
-
json.children[i].data = {}
 
-
json.children[i].children = []
 
-
json.children[i].data.description = 'Methyltransferase: '+site.methyltransferaseDescription
 
-
json.children[i].data.$color = '#0000CD'
 
-
json.children[i].data.length = site.methyltransferaseLength
 
-
json.children[i].data.$angularWidth = 1000
 
-
json.children[i].data.dna = site.methyltransferase
 
-
json.children[i].data.sensor = site.sensor
 
-
json.children[i].name = site.methyltransferaseDescription.split('|')[0]
 
-
json.children[i].id = i
 
-
i++
 
-
json.children[i] = {}
 
-
json.children[i].data = {}
 
-
json.children[i].children = []
 
-
json.children[i].data.description = '120bp random DNA'
 
-
json.children[i].data.$color = '#FFFF00'
 
-
json.children[i].data.length = 120
 
-
json.children[i].data.$angularWidth = 1000
 
-
json.children[i].data.dna = randomDNA(120)
 
-
json.children[i].name = '120bp DNA'
 
-
json.children[i].id = i
 
-
i++
 
-
json.children[i] = {}
 
-
json.children[i].data = {}
 
-
json.children[i].children = []
 
-
json.children[i].data.description = site.zincFingerDescription
 
-
json.children[i].data.$color = '#FF0000'
 
-
json.children[i].data.length = site.zincFingerLength
 
-
json.children[i].data.$angularWidth = 1000
 
-
json.children[i].data.dna = site.zincFinger
 
-
json.children[i].name = site.zincFingerDescription.split('|')[0]+'|'+site.zincFingerDescription.split('|')[1]
 
-
json.children[i].id = i
 
-
i++
 
-
}
 
-
return json
 
-
}
 
-
 
-
function createJSONMemory(number){
 
-
var json = {}
 
-
json = {
 
-
      "children": [],
 
-
      "data": {
 
-
        "$type": "none",
 
-
      },
 
-
      "id": "Source",
 
-
      "name": "Memory Plasmid"
 
-
    };
 
-
 
-
json.children[0] = {}
 
-
json.children[0].data = {}
 
-
json.children[0].data.description = memoryPlasmid.features[1].description
 
-
json.children[0].data.$color = '#B2ABF4'
 
-
json.children[0].data.length = memoryPlasmid.features[1].end - memoryPlasmid.features[1].start
 
-
json.children[0].data.$angularWidth = 1000
 
-
json.children[0].data.type = memoryPlasmid.features[1].type
 
-
json.children[0].data.dna = memoryPlasmid.dna
 
-
json.children[0].children = []
 
-
json.children[0].name = memoryPlasmid.features[1].description
 
-
json.children[0].id = memoryPlasmid.features[1].description
 
-
    i = 1
 
-
    for (memoryObject in memoryPlasmid.memoryObjects){
 
-
for (item in memoryPlasmid.memoryObjects[memoryObject]){
 
-
json.children[i] = {}
 
-
json.children[i].children = []
 
-
json.children[i].data = {}
 
-
json.children[i].data.description = memoryPlasmid.memoryObjects[memoryObject][item].description
 
-
type = memoryPlasmid.memoryObjects[memoryObject][item].type
 
-
if (type == 'Sensor'){
 
-
color = '#00FFFF'
 
-
} else if (type == 'RBS'){
 
-
color = '#FFFF00'
 
-
} else if (type == 'Zinc Finger'){
 
-
color = '#800080'
 
-
} else if (type == 'Linker'){
 
-
color = '#00FF00'
 
-
} else if (type == 'Myc Tag'){
 
-
color = '#FF00FF'
 
-
} else if (type == 'Methyltransferase'){
 
-
color = '#FFA500'
 
-
} else if (type == 'Terminator'){
 
-
color = '#FF0000'
 
-
} else{
 
-
color = '#B2ABF4'
 
-
}
 
-
json.children[i].data.$color = color
 
-
json.children[i].data.length = memoryPlasmid.memoryObjects[memoryObject][item].end - memoryPlasmid.memoryObjects[memoryObject][item].start
 
-
json.children[i].data.$angularWidth = 1000
 
-
json.children[i].data.type = type
 
-
json.children[i].data.dna = memoryPlasmid.memoryObjects[memoryObject][item].sequence
 
-
json.children[i].id = i
 
-
if (memoryPlasmid.memoryObjects[memoryObject][item].description.length > 14){
 
-
json.children[i].name = memoryPlasmid.memoryObjects[memoryObject][item].description.substring(0,14)
 
-
} else{
 
-
json.children[i].name = memoryPlasmid.memoryObjects[memoryObject][item].description
 
-
}
 
-
i++
 
-
}
 
-
}
 
-
return json
 
-
 
 
-
    //end
 
-
}
 
-
 
</script>
</script>
</head>
</head>

Revision as of 19:06, 3 September 2012

Enter your plasmid in fasta or genbank format


Enter your sensors in fasta or genbank format