Team:Potsdam Bioware

From 2012.igem.org

(Difference between revisions)

Revision as of 22:45, 31 August 2012

Home	Team	Official Team Profile	Project	Parts Submitted to the Registry	Modeling	Notebook	Safety	Attributions

Direct Antibody Identification, Maturation and Production in CHO-Cells

Project Description

Antibodies are versatile molecules for research and therapy and they are currently revolutionizing the market of biopharmaceuticals. To date, antibodies are generated in a laborious and time consuming manner. Either animal immunization followed by hybridoma technology or phage display is used to identify desired genes, which then need to be transferred to a production cell line such as CHO. We designed a streamlined workflow that incorporates all steps of antibody generation in CHO cells. Our plan is to stably transfect an antibody-fragment library in CHO cells. Antibody maturation is mimicked by further diversifying this library using the enzyme Activation-Induced cytidine Deaminase (AID), which is known to induce somatic hypermutation. Next, we plan to test and deploy a versatile and continuous viral selection system for clones producing the desired antibodies. Finally, we will try to employ a genetic switch to go from surface expression to soluble production of antibodies.

Team

Mario Köhler

Tom Scheidt

Christopher Kressler

Tobias Priemel

Maria Kunert

Sascha Lehnort

Norman Hein

@@ Line 35: / Line 35: @@
-{|align="justify"
-|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
-|[[Image:Potsdam_Bioware_logo.png|200px|right|frame]]
-|-
-|
-''Tell us more about your project.  Give us background.  Use this as the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
-|[[Image:Potsdam_Bioware_team.png|right|frame|Your team picture]]
-|-
-|
-|align="center"|[[Team:Potsdam_Bioware | Team Potsdam_Bioware]]
-|}
--->
-<!--- The Mission, Experiments --->