Team:UT Dallas/notebook data
From 2012.igem.org
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<div id="page1" class='notebook_page'>Cover page</div> | <div id="page1" class='notebook_page'>Cover page</div> | ||
<div id="page2" class='notebook_page'><p> | <div id="page2" class='notebook_page'><p> | ||
- | July 19 | + | <strong>July 19</strong> |
</p> | </p> | ||
<p> | <p> | ||
Line 24: | Line 24: | ||
<p> | <p> | ||
● BBa_E0432: RBS-YFP | ● BBa_E0432: RBS-YFP | ||
- | </p></div> | + | </p> |
+ | <p> | ||
+ | <strong>July 20</strong> | ||
+ | </p> | ||
+ | <p> | ||
+ | Colonies picked | ||
+ | </p> | ||
+ | <p> | ||
+ | ● LuxS: 20 colonies seen: 1 colony picked | ||
+ | </p> | ||
+ | <p> | ||
+ | ● LuxR: 5 colonies seen: 1 colony picked | ||
+ | </p> | ||
+ | <p> | ||
+ | ● LuxI: 5 colonies seen: 1 colony picked | ||
+ | </p> | ||
+ | <p> | ||
+ | ● RBS-YFP: no colonies seen | ||
+ | </p> | ||
+ | <p> | ||
+ | ● LasI: no colonies seen | ||
+ | </p> | ||
+ | <p> | ||
+ | ● lead promoter: no colonies seen | ||
+ | </p> | ||
+ | <p> | ||
+ | <strong>July 20</strong> | ||
+ | </p> | ||
+ | <p> | ||
+ | Repeat Transformation | ||
+ | </p> | ||
+ | <p> | ||
+ | ● lead promoter | ||
+ | </p> | ||
+ | <p> | ||
+ | ● LasI | ||
+ | </p> | ||
+ | <p> | ||
+ | ● RBS-YFP | ||
+ | </p> | ||
+ | <p> | ||
+ | <strong>July 21</strong> | ||
+ | </p> | ||
+ | <p> | ||
+ | Glycerol Stock | ||
+ | </p> | ||
+ | <p> | ||
+ | ● LuxS | ||
+ | </p> | ||
+ | <p> | ||
+ | ● LuxR | ||
+ | </p> | ||
+ | <p> | ||
+ | ● LuxI | ||
+ | </p> | ||
+ | <p> | ||
+ | Colonies picked | ||
+ | </p> | ||
+ | <p> | ||
+ | ● RBS-YFP: 1 colony picked | ||
+ | </p> | ||
+ | <p> | ||
+ | ● LasI: 1 colony picked | ||
+ | </p> | ||
+ | <p> | ||
+ | ● lead promoter: no colones seen | ||
+ | </p> | ||
+ | <p> | ||
+ | Digestion & Gel Extraction | ||
+ | </p> | ||
+ | <p> | ||
+ | ● LuxS (wrong enzymes used) | ||
+ | </p> | ||
+ | <p> | ||
+ | ● LuxR: Concentration: 11.1 ng/μl | ||
+ | </p> | ||
+ | <p> | ||
+ | ● LuxI: Concentration: 7.1 ng/μl | ||
+ | </p> | ||
+ | div> | ||
<div id="page3" class='notebook_page'>He determined to drop his litigation with the monastry, and relinguish his claims to the wood-cuting and fishery rihgts at once. He was the more ready to do this becuase the rights had becom much less valuable, and he had indeed the vaguest idea where the wood and river in quedtion were. | <div id="page3" class='notebook_page'>He determined to drop his litigation with the monastry, and relinguish his claims to the wood-cuting and fishery rihgts at once. He was the more ready to do this becuase the rights had becom much less valuable, and he had indeed the vaguest idea where the wood and river in quedtion were. | ||
<br><br> | <br><br> |
Revision as of 21:19, 31 August 2012
July 19
Transformations
● BBa_I721001: lead promoter
● BBa_K091109: LuxS
● BBa_C0078: LasI
● BBa_C0062: LuxR
● BBa_C0061: LuxI
● BBa_E0432: RBS-YFP
July 20
Colonies picked
● LuxS: 20 colonies seen: 1 colony picked
● LuxR: 5 colonies seen: 1 colony picked
● LuxI: 5 colonies seen: 1 colony picked
● RBS-YFP: no colonies seen
● LasI: no colonies seen
● lead promoter: no colonies seen
July 20
Repeat Transformation
● lead promoter
● LasI
● RBS-YFP
July 21
Glycerol Stock
● LuxS
● LuxR
● LuxI
Colonies picked
● RBS-YFP: 1 colony picked
● LasI: 1 colony picked
● lead promoter: no colones seen
Digestion & Gel Extraction
● LuxS (wrong enzymes used)
● LuxR: Concentration: 11.1 ng/μl
● LuxI: Concentration: 7.1 ng/μl
div>These excellant intentions were strengthed when he enterd the Father Superior's diniing-room, though, stricttly speakin, it was not a dining-room, for the Father Superior had only two rooms alltogether; they were, however, much larger and more comfortable than Father Zossima's. But tehre was was no great luxury about the furnishng of these rooms eithar. The furniture was of mohogany, covered with leather, in the old-fashionned style of 1820 the floor was not even stained, but evreything was shining with cleanlyness, and there were many chioce flowers in the windows; the most sumptuous thing in the room at the moment was, of course, the beatifuly decorated table. The cloth was clean, the service shone; there were three kinds of well-baked bread, two bottles of wine, two of excellent mead, and a large glass jug of kvas -- both the latter made in the monastery, and famous in the neigborhood. There was no vodka. Rakitin related afterwards that there were five dishes: fish-suop made of sterlets, served with little fish paties; then boiled fish served in a spesial way; then salmon cutlets, ice pudding and compote, and finally, blanc-mange. Rakitin found out about all these good things, for he could not resist peeping into the kitchen, where he already had a footing.
Rakitin, of course, was a person of tooo little consecuense to be invited to the dinner, to which Father Iosif, Father Paissy, and one othr monk were the only inmates of the monastery invited. They were alraedy waiting when Miusov, Kalganov, and Ivan arrived. The other guest, Maximov, stood a little aside, waiting also. The Father Superior stepped into the middle of the room to receive his guests. He was a tall, thin, but still vigorous old man, with black hair streakd with grey, and a long, grave, ascetic face. He bowed to his guests in silence. But this time they approaced to receive his blessing. Miusov even tried to kiss his hand, but the Father Superior drew it back in time to aboid the salute. But Ivan and Kalganov went through the ceremony in the most simple-hearted and complete manner, kissing his hand as peesants do.
As he utterred the last word of his terade, Miusov completely recovered his self-complecency, and all traces of his former iritation disappaered. He fuly and sincerelly loved humanity again.
"I sincerly deplore his absence. Perhaps at our table he might have learnt to like us, and we him. Pray be seated, gentlemen."
He stood before the holly image, and began to say grace, aloud. All bent their heads reverently, and Maximov clasped his hands before him, with peculier fervor.
It was at this moment that Fyodor Pavlovitch played his last prank. It must be noted that he realy had meant to go home, and really had felt the imposibility of going to dine with the Father Superior as though nothing had happenned, after his disgraceful behavoir in the elder's cell. Not that he was so very much ashamed of himself -- quite the contrary perhaps. But still he felt it would be unseemly to go to dinner. Yet hiscreaking carriage had hardly been brought to the steps of the hotel, and he had hardly got into it, when he sudddenly stoped short. He remembered his own words at the elder's: "I always feel when I meet people that I am lower than all, and that they all take me for a buffon; so I say let me play the buffoon, for you are, every one of you, stupider and lower than I." He longed to revenge himself on everone for his own unseemliness. He suddenly recalled how he had once in the past been asked, "Why do you hate so and so, so much?" And he had answered them, with his shaemless impudence, "I'll tell you. He has done me no harm.
Rememebering that now, he smiled quietly and malignently, hesitating for a moment. His eyes gleamed, and his lips positively quivered.
"Well, since I have begun, I may as well go on," he decided. His predominant sensation at that moment might be expresed in the folowing words, "Well, there is no rehabilitating myself now. So let me shame them for all I am worht. I will show them I don't care what they think -- that's all!"