Team:Macquarie Australia/Protocols/ArrivalofGBlocks

From 2012.igem.org

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! scope="row"| Agrobacterium (T7 minus)
! scope="row"| Agrobacterium (T7 minus)
| Agro_A + Agro_B + Agro_C + Agro_D + Agro_E
| Agro_A + Agro_B + Agro_C + Agro_D + Agro_E
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|}
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Once the Deinococcus Radiodurans fragments arrive we will assemble them using Gibson assembly. Due to the large sequence of the D. radiodurans bacteriophytochrome, the fragments will be assembled without a T7 promoter.
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{| class="wikitable"
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! scope="col"| BioBrick name
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! scope="col"| Fragment(s)
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! scope="row"| Deinococcus radiodurans bacteriophytochrome
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| Deino_A + Deino_B + Deino_C + Deino_D + Deino_E
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Revision as of 08:38, 31 August 2012



Ordered gBlock fragments are nearly here!

Soon our gBlock fragments will arrive from IDT. On Tuesday (4/09/2012) we will perform Gibson assembly will assemble the following four biobricks:

Click on each above to see the corresponding fragment nucleotide sequence and fragment properties.


BioBrick name Fragment(s)
T7 Heme oxygenase Hemo_T7_A + Hemo_B + Plasmid
Heme oxygenase (T7 minus) Hemo_A + Hemo_B + Plasmid
T7 Agrobacterium Agro_T7_A + Agro_B + Agro_C + Agro_D + Agro_E + Plasmid
Agrobacterium (T7 minus) Agro_A + Agro_B + Agro_C + Agro_D + Agro_E


Once the Deinococcus Radiodurans fragments arrive we will assemble them using Gibson assembly. Due to the large sequence of the D. radiodurans bacteriophytochrome, the fragments will be assembled without a T7 promoter.

BioBrick name Fragment(s)
Deinococcus radiodurans bacteriophytochrome Deino_A + Deino_B + Deino_C + Deino_D + Deino_E
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