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Team:Macquarie Australia/Protocols/ArrivalofGBlocks
From 2012.igem.org
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Soon our gBlock fragments will arrive from IDT. On Tuesday (4/09/2012) we will perform Gibson assembly will assemble the following four biobricks: | Soon our gBlock fragments will arrive from IDT. On Tuesday (4/09/2012) we will perform Gibson assembly will assemble the following four biobricks: | ||
| - | * [[media:AgroT7A.pdf | +T7 promoter Agrobacterium tumefaciens bacteriophytochrome ]] | + | * [[media:AgroT7A.pdf |+T7 promoter Agrobacterium tumefaciens bacteriophytochrome ]] |
| - | * [[media:AgroA.pdf | -T7 promoter Agrobacterium tumefaciens bacteriophytochrome ]] | + | * [[media:AgroA.pdf |-T7 promoter Agrobacterium tumefaciens bacteriophytochrome ]] |
| - | * [[media:T7Heme.pdf | +T7 promoter Heme oxygenase ]] | + | * [[media:T7Heme.pdf |+T7 promoter Heme oxygenase ]] |
| - | * [[media:HemeA.pdf | -T7 promoter Heme oxygenase ]] | + | * [[media:HemeA.pdf |-T7 promoter Heme oxygenase ]] |
Click on each above to see the corresponding fragment nucleotide sequence and fragment properties. | Click on each above to see the corresponding fragment nucleotide sequence and fragment properties. | ||
Revision as of 08:33, 31 August 2012
Ordered gBlock fragments are nearly here!
Soon our gBlock fragments will arrive from IDT. On Tuesday (4/09/2012) we will perform Gibson assembly will assemble the following four biobricks:
- +T7 promoter Agrobacterium tumefaciens bacteriophytochrome
- -T7 promoter Agrobacterium tumefaciens bacteriophytochrome
- +T7 promoter Heme oxygenase
- -T7 promoter Heme oxygenase
Click on each above to see the corresponding fragment nucleotide sequence and fragment properties.
| BioBrick name | Fragment(s) |
|---|---|
| T7 Heme oxygenase | Hemo_T7_A + Hemo_B + Plasmid |
| Heme oxygenase (T7 minus) | Hemo_A + Hemo_B + Plasmid |
| T7 Agrobacterium | Agro_T7_A + Agro_B + Agro_C + Agro_D + Agro_E + Plasmid |
| Agrobacterium (T7 minus) | Agro_A + Agro_B + Agro_C + Agro_D + Agro_E |
