"
Team:Potsdam Bioware
From 2012.igem.org
(→Project Description) |
(→Project Description) |
||
| Line 95: | Line 95: | ||
</style> | </style> | ||
</head> | </head> | ||
| + | <body>sss | ||
| + | </body> | ||
</html> | </html> | ||
Revision as of 20:53, 24 August 2012
Direct Antibody Identification, Maturation and Production in CHO-Cells
Project Description
Antibodies are versatile molecules for research and therapy and they are currently revolutionizing the market of biopharmaceuticals. To date, antibodies are generated in a laborious and time consuming manner. Either animal immunization followed by hybridoma technology or phage display is used to identify desired genes, which then need to be transferred to a production cell line such as CHO. We designed a streamlined workflow that incorporates all steps of antibody generation in CHO cells. Our plan is to stably transfect an antibody-fragment library in CHO cells. Antibody maturation is mimicked by further diversifying this library using the enzyme Activation-Induced cytidine Deaminase (AID), which is known to induce somatic hypermutation. Next, we plan to test and deploy a versatile and continuous viral selection system for clones producing the desired antibodies. Finally, we will try to employ a genetic switch to go from surface expression to soluble production of antibodies.
| Home | Team | Official Team Profile | Project | Parts Submitted to the Registry | Modeling | Notebook | Safety | Attributions |
|---|
sss
