Team:Potsdam Bioware

From 2012.igem.org

(Difference between revisions)

Revision as of 20:51, 24 August 2012

Direct Antibody Identification, Maturation and Production in CHO-Cells

Project Description

Antibodies are versatile molecules for research and therapy and they are currently revolutionizing the market of biopharmaceuticals. To date, antibodies are generated in a laborious and time consuming manner. Either animal immunization followed by hybridoma technology or phage display is used to identify desired genes, which then need to be transferred to a production cell line such as CHO. We designed a streamlined workflow that incorporates all steps of antibody generation in CHO cells. Our plan is to stably transfect an antibody-fragment library in CHO cells. Antibody maturation is mimicked by further diversifying this library using the enzyme Activation-Induced cytidine Deaminase (AID), which is known to induce somatic hypermutation. Next, we plan to test and deploy a versatile and continuous viral selection system for clones producing the desired antibodies. Finally, we will try to employ a genetic switch to go from surface expression to soluble production of antibodies.

Home	Team	Official Team Profile	Project	Parts Submitted to the Registry	Modeling	Notebook	Safety	Attributions

@@ Line 70: / Line 70: @@
 body {
-   background: repeat-y;
+/* IE10 Consumer Preview */
-   background: rgb(255,255,255);
+background-image: -ms-linear-gradient(top, #FFFFFF 0%, #999999 100%);
-   background: -moz-linear-gradient(top,  rgba(255,255,255,1) 16%, rgba(153,153,153,1) 100%);
-   background: -webkit-gradient(linear, left top, left bottom, color-stop(16%,rgba(255,255,255,1)), color-stop(100%,rgb(153,153,153,1)));
-   background: -webkit-linear-gradient(top,  rgba(255,255,255,1) 16%,rgba(153,153,153,1) 100%);
-   background: -o-linear-gradient(top,  rgba(255,255,255,1) 16%,rgba(153,153,153,1) 100%);
-   background: -ms-linear-gradient(top,  rgba(255,255,255,1) 16%,rgba(153,153,153,1) 100%);
-   background: linear-gradient(to bottom,  rgba(255,255,255,1) 16%,rgba(153,153,153,1) 100%);
-   filter: progid:DXImageTransform.Microsoft.gradient( startColorstr='#ffffff', endColorstr='#999999',GradientType=0 );
+/* Mozilla Firefox */
+background-image: -moz-linear-gradient(top, #FFFFFF 0%, #999999 100%);
+/* Opera */
+background-image: -o-linear-gradient(top, #FFFFFF 0%, #999999 100%);
+/* Webkit (Safari/Chrome 10) */
+background-image: -webkit-gradient(linear, left top, left bottom, color-stop(0, #FFFFFF), color-stop(1, #999999));
+/* Webkit (Chrome 11+) */
+background-image: -webkit-linear-gradient(top, #FFFFFF 0%, #999999 100%);
+/* W3C Markup, IE10 Release Preview */
+background-image: linear-gradient(to bottom, #FFFFFF 0%, #999999 100%);
 }