Team:Potsdam Bioware

From 2012.igem.org

(Difference between revisions)

Revision as of 20:47, 24 August 2012

Direct Antibody Identification, Maturation and Production in CHO-Cells

Project Description

Antibodies are versatile molecules for research and therapy and they are currently revolutionizing the market of biopharmaceuticals. To date, antibodies are generated in a laborious and time consuming manner. Either animal immunization followed by hybridoma technology or phage display is used to identify desired genes, which then need to be transferred to a production cell line such as CHO. We designed a streamlined workflow that incorporates all steps of antibody generation in CHO cells. Our plan is to stably transfect an antibody-fragment library in CHO cells. Antibody maturation is mimicked by further diversifying this library using the enzyme Activation-Induced cytidine Deaminase (AID), which is known to induce somatic hypermutation. Next, we plan to test and deploy a versatile and continuous viral selection system for clones producing the desired antibodies. Finally, we will try to employ a genetic switch to go from surface expression to soluble production of antibodies.

Home	Team	Official Team Profile	Project	Parts Submitted to the Registry	Modeling	Notebook	Safety	Attributions

@@ Line 71: / Line 71: @@
 body {
-    background-color: green;
+    background: repeat-x
+   background: rgb(255,255,255);
+   background: -moz-linear-gradient(top,  rgba(255,255,255,1) 16%, rgba(153,153,153,1) 100%);
+   background: -webkit-gradient(linear, left top, left bottom, color-stop(16%,rgba(255,255,255,1)), color-stop(100%,rgb(153,153,153,1)));
+   background: -webkit-linear-gradient(top,  rgba(255,255,255,1) 16%,rgba(153,153,153,1) 100%);
+   background: -o-linear-gradient(top,  rgba(255,255,255,1) 16%,rgba(153,153,153,1) 100%);
+   background: -ms-linear-gradient(top,  rgba(255,255,255,1) 16%,rgba(153,153,153,1) 100%);
+   background: linear-gradient(to bottom,  rgba(255,255,255,1) 16%,rgba(153,153,153,1) 100%);
+   filter: progid:DXImageTransform.Microsoft.gradient( startColorstr='#ffffff', endColorstr='#999999',GradientType=0 );
 }