Team:LMU-Munich/Lab Notebook

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==Lab Notebook==
==Lab Notebook==
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See our [[Team:LMU-Munich/Weekly_Journal |Weekly Journal]] for details about our progress.
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<col class=xl65535 width=64 span=2 style='mso-width-source:userset;mso-width-alt:
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2730;width:64pt'>
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<col class=xl65535 width=77 style='mso-width-source:userset;mso-width-alt:
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3285;width:77pt'>
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<tr height=15 style='height:15.0pt'>
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  <td height=15 class=xl65 width=426 style='height:15.0pt;width:426pt'>Accomplishment</td>
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  <td class=xl66 width=63 style='border-left:none;width:63pt'>Date</td>
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  <td class=xl67 width=34 style='border-left:none;width:34pt'>Week</td>
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  <td class=xl67 width=64 style='border-left:none;width:64pt'>Project</td>
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  <td class=xl67 width=64 style='border-left:none;width:64pt'>Your name</td>
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  <td class=xl72 width=77 style='border-left:none;width:77pt'>Data</td>
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</tr>
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<tr class=xl68 height=29 style='height:29.0pt'>
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  <td height=29 class=xl70 width=426 style='height:29.0pt;width:426pt'>E.g.
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-
  Created double-mutant using resistance-cassette KOs:<font class="font6"> cwlD</font><font
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  class="font0">::kan+</font><font class="font6">gerD</font><font class="font0">::cat</font></td>
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  <td class=xl71 width=63 style='border-left:none;width:63pt'>8.8.2012</td>
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  <td class=xl70 width=34 style='border-left:none;width:34pt'>32</td>
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  <td class=xl70 width=64 style='border-left:none;width:64pt'>Germination<font
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  class="font5"><span style="mso-spacerun:yes">&nbsp;</span>Stop</font></td>
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  <td class=xl70 width=64 style='border-left:none;width:64pt'>Jenny &amp; Jara</td>
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  <td class=xl70 width=77 style='border-left:none;width:77pt'>&nbsp;</td>
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</tr>
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<tr class=xl68 height=29 style='height:29.0pt'>
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  <td height=29 class=xl68 width=426 style='height:29.0pt;width:426pt'>First
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  Single mutant: sleB::MLS</td>
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  <td class=xl69 width=63 style='border-left:none;width:63pt'>4.2.2012</td>
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  <td class=xl68 width=34 style='border-left:none;width:34pt'>14</td>
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  <td class=xl68 width=64 style='border-left:none;width:64pt'>Germination<font
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-
  class="font5"><span style="mso-spacerun:yes">&nbsp;</span>Stop</font></td>
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-
  <td class=xl68 width=64 style='border-left:none;width:64pt'>Jara</td>
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-
  <td class=xl68 width=77 style='border-left:none;width:77pt'>&nbsp;</td>
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-
</tr>
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-
<tr class=xl68 height=29 style='height:29.0pt'>
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-
  <td height=29 class=xl68 width=426 style='height:29.0pt;width:426pt'>Single
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-
  mutant: cwlJ::spec</td>
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-
  <td class=xl69 width=63 style='border-left:none;width:63pt'>4.2.2012</td>
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-
  <td class=xl68 width=34 style='border-left:none;width:34pt'>14</td>
+
-
  <td class=xl68 width=64 style='border-left:none;width:64pt'>Germination<font
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-
  class="font5"><span style="mso-spacerun:yes">&nbsp;</span>Stop</font></td>
+
-
  <td class=xl68 width=64 style='border-left:none;width:64pt'>Jara</td>
+
-
  <td class=xl68 width=77 style='border-left:none;width:77pt'>&nbsp;</td>
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-
</tr>
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-
<tr class=xl68 height=29 style='height:29.0pt'>
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-
  <td height=29 class=xl68 width=426 style='height:29.0pt;width:426pt'>Single
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  mutant: gerD::cat</td>
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  <td class=xl69 width=63 style='border-left:none;width:63pt'>4.4.2012</td>
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  <td class=xl68 width=34 style='border-left:none;width:34pt'>14</td>
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  <td class=xl68 width=64 style='border-left:none;width:64pt'>Germination<font
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  class="font5"><span style="mso-spacerun:yes">&nbsp;</span>Stop</font></td>
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  <td class=xl68 width=64 style='border-left:none;width:64pt'>Jara</td>
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  <td class=xl68 width=77 style='border-left:none;width:77pt'>&nbsp;</td>
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-
</tr>
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-
<tr class=xl68 height=29 style='height:29.0pt'>
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-
  <td height=29 class=xl68 width=426 style='height:29.0pt;width:426pt'>First
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  Bacillus iGEM backbone! pSBBS0K-Pspac (from: pDG148-RFP)</td>
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  <td class=xl69 width=63 style='border-left:none;width:63pt'>4.5.2012</td>
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  <td class=xl68 width=34 style='border-left:none;width:34pt'>14</td>
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  <td class=xl68 width=64 style='border-left:none;width:64pt'>Bacillus BioBrick<font
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  class="font5">Box</font></td>
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-
  <td class=xl68 width=64 style='border-left:none;width:64pt'>Jara</td>
+
-
  <td class=xl68 width=77 style='border-left:none;width:77pt'>&nbsp;</td>
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-
</tr>
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-
<tr class=xl68 height=29 style='height:29.0pt'>
+
-
  <td height=29 class=xl68 width=426 style='height:29.0pt;width:426pt'>Single
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-
  mutant cwlD::kann</td>
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-
  <td class=xl69 width=63 style='border-left:none;width:63pt'>4.10.2012</td>
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-
  <td class=xl68 width=34 style='border-left:none;width:34pt'>15</td>
+
-
  <td class=xl68 width=64 style='border-left:none;width:64pt'>Germination<font
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-
  class="font5"><span style="mso-spacerun:yes">&nbsp;</span>Stop</font></td>
+
-
  <td class=xl68 width=64 style='border-left:none;width:64pt'>Jara</td>
+
-
  <td class=xl68 width=77 style='border-left:none;width:77pt'>&nbsp;</td>
+
-
</tr>
+
-
<tr class=xl68 height=15 style='height:15.0pt'>
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-
  <td height=15 class=xl68 width=426 style='height:15.0pt;width:426pt'>GFP-spore
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-
  plasmids from Japan arrived</td>
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-
  <td class=xl69 width=63 style='border-left:none;width:63pt'>4.13.2012</td>
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  <td class=xl68 width=34 style='border-left:none;width:34pt'>15</td>
+
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  <td class=xl68 width=64 style='border-left:none;width:64pt'>Sporo<font
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  class="font5">beads</font></td>
+
-
  <td class=xl68 width=64 style='border-left:none;width:64pt'>&nbsp;</td>
+
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  <td class=xl68 width=77 style='border-left:none;width:77pt'>&nbsp;</td>
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</tr>
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<tr class=xl68 height=29 style='height:29.0pt'>
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-
  <td height=29 class=xl68 width=426 style='height:29.0pt;width:426pt'>Bacillus
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  pSBBS (pAH328-RFP)</td>
+
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  <td class=xl69 width=63 style='border-left:none;width:63pt'>&nbsp;</td>
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-
  <td class=xl68 width=34 style='border-left:none;width:34pt'>0</td>
+
-
  <td class=xl68 width=64 style='border-left:none;width:64pt'>Bacillus BioBrick<font
+
-
  class="font5">Box</font></td>
+
-
  <td class=xl68 width=64 style='border-left:none;width:64pt'>Jara</td>
+
-
  <td class=xl68 width=77 style='border-left:none;width:77pt'>&nbsp;</td>
+
-
</tr>
+
-
<tr class=xl68 height=29 style='height:29.0pt'>
+
-
  <td height=29 class=xl68 width=426 style='height:29.0pt;width:426pt'>pSBBS1C-lacZ
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-
  (pAC6-RFP)</td>
+
-
  <td class=xl69 width=63 style='border-left:none;width:63pt'>4.19.2012</td>
+
-
  <td class=xl68 width=34 style='border-left:none;width:34pt'>16</td>
+
-
  <td class=xl68 width=64 style='border-left:none;width:64pt'>Bacillus BioBrick<font
+
-
  class="font5">Box</font></td>
+
-
  <td class=xl68 width=64 style='border-left:none;width:64pt'>Jara</td>
+
-
  <td class=xl68 width=77 style='border-left:none;width:77pt'>&nbsp;</td>
+
-
</tr>
+
-
<tr class=xl68 height=29 style='height:29.0pt'>
+
-
  <td height=29 class=xl68 width=426 style='height:29.0pt;width:426pt'>pSBBS4S-Pxyl
+
-
  (pXT-RFP)</td>
+
-
  <td class=xl69 width=63 style='border-left:none;width:63pt'>7.9.2012</td>
+
-
  <td class=xl68 width=34 style='border-left:none;width:34pt'>28</td>
+
-
  <td class=xl68 width=64 style='border-left:none;width:64pt'>Bacillus BioBrick<font
+
-
  class="font5">Box</font></td>
+
-
  <td class=xl68 width=64 style='border-left:none;width:64pt'>Jara</td>
+
-
  <td class=xl68 width=77 style='border-left:none;width:77pt'>&nbsp;</td>
+
-
</tr>
+
-
<tr class=xl68 height=29 style='height:29.0pt'>
+
-
  <td height=29 class=xl68 width=426 style='height:29.0pt;width:426pt'>First
+
-
  empty Bacillus backbone pSBBS1C (pDG1662-RFP)</td>
+
-
  <td class=xl69 width=63 style='border-left:none;width:63pt'>7.9.2012</td>
+
-
  <td class=xl68 width=34 style='border-left:none;width:34pt'>28</td>
+
-
  <td class=xl68 width=64 style='border-left:none;width:64pt'>Bacillus BioBrick<font
+
-
  class="font5">Box</font></td>
+
-
  <td class=xl68 width=64 style='border-left:none;width:64pt'>Jara</td>
+
-
  <td class=xl68 width=77 style='border-left:none;width:77pt'>&nbsp;</td>
+
-
</tr>
+
-
<tr class=xl68 height=29 style='height:29.0pt'>
+
-
  <td height=29 class=xl68 width=426 style='height:29.0pt;width:426pt'>pSBBS4S
+
-
  (pDG1731-RFP)</td>
+
-
  <td class=xl69 width=63 style='border-left:none;width:63pt'>7.9.2012</td>
+
-
  <td class=xl68 width=34 style='border-left:none;width:34pt'>28</td>
+
-
  <td class=xl68 width=64 style='border-left:none;width:64pt'>Bacillus BioBrick<font
+
-
  class="font5">Box</font></td>
+
-
  <td class=xl68 width=64 style='border-left:none;width:64pt'>Jara</td>
+
-
  <td class=xl68 width=77 style='border-left:none;width:77pt'>&nbsp;</td>
+
-
</tr>
+
-
<tr class=xl68 height=43 style='height:43.0pt'>
+
-
  <td height=43 class=xl68 width=426 style='height:43.0pt;width:426pt'>Double
+
-
  negative loop with lacZa finished (works qualitativly</td>
+
-
  <td class=xl69 width=63 style='border-left:none;width:63pt'>8.22.2012</td>
+
-
  <td class=xl68 width=34 style='border-left:none;width:34pt'>34</td>
+
-
  <td class=xl68 width=64 style='border-left:none;width:64pt'>double negative
+
-
  loop</td>
+
-
  <td class=xl68 width=64 style='border-left:none;width:64pt'>Julia</td>
+
-
  <td class=xl68 width=77 style='border-left:none;width:77pt'>&nbsp;</td>
+
-
</tr>
+
-
<tr class=xl68 height=15 style='height:15.0pt'>
+
-
  <td height=15 class=xl68 width=426 style='height:15.0pt;width:426pt'>&nbsp;</td>
+
-
  <td class=xl69 width=63 style='border-left:none;width:63pt'>&nbsp;</td>
+
-
  <td class=xl68 width=34 style='border-left:none;width:34pt'>&nbsp;</td>
+
-
  <td class=xl68 width=64 style='border-left:none;width:64pt'>&nbsp;</td>
+
-
  <td class=xl68 width=64 style='border-left:none;width:64pt'>&nbsp;</td>
+
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  <td class=xl68 width=77 style='border-left:none;width:77pt'>&nbsp;</td>
+
-
</tr>
+
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<tr class=xl68 height=15 style='height:15.0pt'>
+
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  <td height=15 class=xl68 width=426 style='height:15.0pt;width:426pt'>&nbsp;</td>
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  <td class=xl69 width=63 style='border-left:none;width:63pt'>&nbsp;</td>
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-
  <td class=xl68 width=34 style='border-left:none;width:34pt'>&nbsp;</td>
+
-
  <td class=xl68 width=64 style='border-left:none;width:64pt'>&nbsp;</td>
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  <td class=xl68 width=64 style='border-left:none;width:64pt'>&nbsp;</td>
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  <td class=xl68 width=34 style='border-left:none;width:34pt'>&nbsp;</td>
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  <td class=xl68 width=64 style='border-left:none;width:64pt'>&nbsp;</td>
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See our [[Team:LMU-Munich/Data |Data page]] to see all our results.
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See our [[Team:LMU-Munich/Lab_Notebook/Protocols |Protocols Page]] to download a description of our methods. This is particular interesting if you want to start working with Bacillus subtilis as well.
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{{:Team:LMU-Munich/Templates/Page Footer}}

Revision as of 13:23, 23 August 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

Team-LMU Photo8.jpg

The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

IGEM HQ LMU prize.jpg

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