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| ==Lab Notebook== | | ==Lab Notebook== |
| | | |
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| + | See our [[Team:LMU-Munich/Weekly_Journal |Weekly Journal]] for details about our progress. |
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| + | |
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| + | |
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| + | |
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| + | |
- | 18176;width:426pt'>
| + | |
- | <col class=xl65535 width=63 style='mso-width-source:userset;mso-width-alt:
| + | |
- | 2688;width:63pt'>
| + | |
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| + | |
- | <tr height=15 style='height:15.0pt'>
| + | |
- | <td height=15 class=xl65 width=426 style='height:15.0pt;width:426pt'>Accomplishment</td>
| + | |
- | <td class=xl66 width=63 style='border-left:none;width:63pt'>Date</td>
| + | |
- | <td class=xl67 width=34 style='border-left:none;width:34pt'>Week</td>
| + | |
- | <td class=xl67 width=64 style='border-left:none;width:64pt'>Project</td>
| + | |
- | <td class=xl67 width=64 style='border-left:none;width:64pt'>Your name</td>
| + | |
- | <td class=xl72 width=77 style='border-left:none;width:77pt'>Data</td>
| + | |
- | </tr>
| + | |
- | <tr class=xl68 height=29 style='height:29.0pt'>
| + | |
- | <td height=29 class=xl70 width=426 style='height:29.0pt;width:426pt'>E.g.
| + | |
- | Created double-mutant using resistance-cassette KOs:<font class="font6"> cwlD</font><font
| + | |
- | class="font0">::kan+</font><font class="font6">gerD</font><font class="font0">::cat</font></td>
| + | |
- | <td class=xl71 width=63 style='border-left:none;width:63pt'>8.8.2012</td>
| + | |
- | <td class=xl70 width=34 style='border-left:none;width:34pt'>32</td>
| + | |
- | <td class=xl70 width=64 style='border-left:none;width:64pt'>Germination<font
| + | |
- | class="font5"><span style="mso-spacerun:yes"> </span>Stop</font></td>
| + | |
- | <td class=xl70 width=64 style='border-left:none;width:64pt'>Jenny & Jara</td>
| + | |
- | <td class=xl70 width=77 style='border-left:none;width:77pt'> </td>
| + | |
- | </tr>
| + | |
- | <tr class=xl68 height=29 style='height:29.0pt'>
| + | |
- | <td height=29 class=xl68 width=426 style='height:29.0pt;width:426pt'>First
| + | |
- | Single mutant: sleB::MLS</td>
| + | |
- | <td class=xl69 width=63 style='border-left:none;width:63pt'>4.2.2012</td>
| + | |
- | <td class=xl68 width=34 style='border-left:none;width:34pt'>14</td>
| + | |
- | <td class=xl68 width=64 style='border-left:none;width:64pt'>Germination<font
| + | |
- | class="font5"><span style="mso-spacerun:yes"> </span>Stop</font></td>
| + | |
- | <td class=xl68 width=64 style='border-left:none;width:64pt'>Jara</td>
| + | |
- | <td class=xl68 width=77 style='border-left:none;width:77pt'> </td>
| + | |
- | </tr>
| + | |
- | <tr class=xl68 height=29 style='height:29.0pt'>
| + | |
- | <td height=29 class=xl68 width=426 style='height:29.0pt;width:426pt'>Single
| + | |
- | mutant: cwlJ::spec</td>
| + | |
- | <td class=xl69 width=63 style='border-left:none;width:63pt'>4.2.2012</td>
| + | |
- | <td class=xl68 width=34 style='border-left:none;width:34pt'>14</td>
| + | |
- | <td class=xl68 width=64 style='border-left:none;width:64pt'>Germination<font
| + | |
- | class="font5"><span style="mso-spacerun:yes"> </span>Stop</font></td>
| + | |
- | <td class=xl68 width=64 style='border-left:none;width:64pt'>Jara</td>
| + | |
- | <td class=xl68 width=77 style='border-left:none;width:77pt'> </td>
| + | |
- | </tr>
| + | |
- | <tr class=xl68 height=29 style='height:29.0pt'>
| + | |
- | <td height=29 class=xl68 width=426 style='height:29.0pt;width:426pt'>Single
| + | |
- | mutant: gerD::cat</td>
| + | |
- | <td class=xl69 width=63 style='border-left:none;width:63pt'>4.4.2012</td>
| + | |
- | <td class=xl68 width=34 style='border-left:none;width:34pt'>14</td>
| + | |
- | <td class=xl68 width=64 style='border-left:none;width:64pt'>Germination<font
| + | |
- | class="font5"><span style="mso-spacerun:yes"> </span>Stop</font></td>
| + | |
- | <td class=xl68 width=64 style='border-left:none;width:64pt'>Jara</td>
| + | |
- | <td class=xl68 width=77 style='border-left:none;width:77pt'> </td>
| + | |
- | </tr>
| + | |
- | <tr class=xl68 height=29 style='height:29.0pt'>
| + | |
- | <td height=29 class=xl68 width=426 style='height:29.0pt;width:426pt'>First
| + | |
- | Bacillus iGEM backbone! pSBBS0K-Pspac (from: pDG148-RFP)</td>
| + | |
- | <td class=xl69 width=63 style='border-left:none;width:63pt'>4.5.2012</td>
| + | |
- | <td class=xl68 width=34 style='border-left:none;width:34pt'>14</td>
| + | |
- | <td class=xl68 width=64 style='border-left:none;width:64pt'>Bacillus BioBrick<font
| + | |
- | class="font5">Box</font></td>
| + | |
- | <td class=xl68 width=64 style='border-left:none;width:64pt'>Jara</td>
| + | |
- | <td class=xl68 width=77 style='border-left:none;width:77pt'> </td>
| + | |
- | </tr>
| + | |
- | <tr class=xl68 height=29 style='height:29.0pt'>
| + | |
- | <td height=29 class=xl68 width=426 style='height:29.0pt;width:426pt'>Single
| + | |
- | mutant cwlD::kann</td>
| + | |
- | <td class=xl69 width=63 style='border-left:none;width:63pt'>4.10.2012</td>
| + | |
- | <td class=xl68 width=34 style='border-left:none;width:34pt'>15</td>
| + | |
- | <td class=xl68 width=64 style='border-left:none;width:64pt'>Germination<font
| + | |
- | class="font5"><span style="mso-spacerun:yes"> </span>Stop</font></td>
| + | |
- | <td class=xl68 width=64 style='border-left:none;width:64pt'>Jara</td>
| + | |
- | <td class=xl68 width=77 style='border-left:none;width:77pt'> </td>
| + | |
- | </tr>
| + | |
- | <tr class=xl68 height=15 style='height:15.0pt'>
| + | |
- | <td height=15 class=xl68 width=426 style='height:15.0pt;width:426pt'>GFP-spore
| + | |
- | plasmids from Japan arrived</td>
| + | |
- | <td class=xl69 width=63 style='border-left:none;width:63pt'>4.13.2012</td>
| + | |
- | <td class=xl68 width=34 style='border-left:none;width:34pt'>15</td>
| + | |
- | <td class=xl68 width=64 style='border-left:none;width:64pt'>Sporo<font
| + | |
- | class="font5">beads</font></td>
| + | |
- | <td class=xl68 width=64 style='border-left:none;width:64pt'> </td>
| + | |
- | <td class=xl68 width=77 style='border-left:none;width:77pt'> </td>
| + | |
- | </tr>
| + | |
- | <tr class=xl68 height=29 style='height:29.0pt'>
| + | |
- | <td height=29 class=xl68 width=426 style='height:29.0pt;width:426pt'>Bacillus
| + | |
- | pSBBS (pAH328-RFP)</td>
| + | |
- | <td class=xl69 width=63 style='border-left:none;width:63pt'> </td>
| + | |
- | <td class=xl68 width=34 style='border-left:none;width:34pt'>0</td>
| + | |
- | <td class=xl68 width=64 style='border-left:none;width:64pt'>Bacillus BioBrick<font
| + | |
- | class="font5">Box</font></td>
| + | |
- | <td class=xl68 width=64 style='border-left:none;width:64pt'>Jara</td>
| + | |
- | <td class=xl68 width=77 style='border-left:none;width:77pt'> </td>
| + | |
- | </tr>
| + | |
- | <tr class=xl68 height=29 style='height:29.0pt'>
| + | |
- | <td height=29 class=xl68 width=426 style='height:29.0pt;width:426pt'>pSBBS1C-lacZ
| + | |
- | (pAC6-RFP)</td>
| + | |
- | <td class=xl69 width=63 style='border-left:none;width:63pt'>4.19.2012</td>
| + | |
- | <td class=xl68 width=34 style='border-left:none;width:34pt'>16</td>
| + | |
- | <td class=xl68 width=64 style='border-left:none;width:64pt'>Bacillus BioBrick<font
| + | |
- | class="font5">Box</font></td>
| + | |
- | <td class=xl68 width=64 style='border-left:none;width:64pt'>Jara</td>
| + | |
- | <td class=xl68 width=77 style='border-left:none;width:77pt'> </td>
| + | |
- | </tr>
| + | |
- | <tr class=xl68 height=29 style='height:29.0pt'>
| + | |
- | <td height=29 class=xl68 width=426 style='height:29.0pt;width:426pt'>pSBBS4S-Pxyl
| + | |
- | (pXT-RFP)</td>
| + | |
- | <td class=xl69 width=63 style='border-left:none;width:63pt'>7.9.2012</td>
| + | |
- | <td class=xl68 width=34 style='border-left:none;width:34pt'>28</td>
| + | |
- | <td class=xl68 width=64 style='border-left:none;width:64pt'>Bacillus BioBrick<font
| + | |
- | class="font5">Box</font></td>
| + | |
- | <td class=xl68 width=64 style='border-left:none;width:64pt'>Jara</td>
| + | |
- | <td class=xl68 width=77 style='border-left:none;width:77pt'> </td>
| + | |
- | </tr>
| + | |
- | <tr class=xl68 height=29 style='height:29.0pt'>
| + | |
- | <td height=29 class=xl68 width=426 style='height:29.0pt;width:426pt'>First
| + | |
- | empty Bacillus backbone pSBBS1C (pDG1662-RFP)</td>
| + | |
- | <td class=xl69 width=63 style='border-left:none;width:63pt'>7.9.2012</td>
| + | |
- | <td class=xl68 width=34 style='border-left:none;width:34pt'>28</td>
| + | |
- | <td class=xl68 width=64 style='border-left:none;width:64pt'>Bacillus BioBrick<font
| + | |
- | class="font5">Box</font></td>
| + | |
- | <td class=xl68 width=64 style='border-left:none;width:64pt'>Jara</td>
| + | |
- | <td class=xl68 width=77 style='border-left:none;width:77pt'> </td>
| + | |
- | </tr>
| + | |
- | <tr class=xl68 height=29 style='height:29.0pt'>
| + | |
- | <td height=29 class=xl68 width=426 style='height:29.0pt;width:426pt'>pSBBS4S
| + | |
- | (pDG1731-RFP)</td>
| + | |
- | <td class=xl69 width=63 style='border-left:none;width:63pt'>7.9.2012</td>
| + | |
- | <td class=xl68 width=34 style='border-left:none;width:34pt'>28</td>
| + | |
- | <td class=xl68 width=64 style='border-left:none;width:64pt'>Bacillus BioBrick<font
| + | |
- | class="font5">Box</font></td>
| + | |
- | <td class=xl68 width=64 style='border-left:none;width:64pt'>Jara</td>
| + | |
- | <td class=xl68 width=77 style='border-left:none;width:77pt'> </td>
| + | |
- | </tr>
| + | |
- | <tr class=xl68 height=43 style='height:43.0pt'>
| + | |
- | <td height=43 class=xl68 width=426 style='height:43.0pt;width:426pt'>Double
| + | |
- | negative loop with lacZa finished (works qualitativly</td>
| + | |
- | <td class=xl69 width=63 style='border-left:none;width:63pt'>8.22.2012</td>
| + | |
- | <td class=xl68 width=34 style='border-left:none;width:34pt'>34</td>
| + | |
- | <td class=xl68 width=64 style='border-left:none;width:64pt'>double negative
| + | |
- | loop</td>
| + | |
- | <td class=xl68 width=64 style='border-left:none;width:64pt'>Julia</td>
| + | |
- | <td class=xl68 width=77 style='border-left:none;width:77pt'> </td>
| + | |
- | </tr>
| + | |
- | <tr class=xl68 height=15 style='height:15.0pt'>
| + | |
- | <td height=15 class=xl68 width=426 style='height:15.0pt;width:426pt'> </td>
| + | |
- | <td class=xl69 width=63 style='border-left:none;width:63pt'> </td>
| + | |
- | <td class=xl68 width=34 style='border-left:none;width:34pt'> </td>
| + | |
- | <td class=xl68 width=64 style='border-left:none;width:64pt'> </td>
| + | |
- | <td class=xl68 width=64 style='border-left:none;width:64pt'> </td>
| + | |
- | <td class=xl68 width=77 style='border-left:none;width:77pt'> </td>
| + | |
- | </tr>
| + | |
- | <tr class=xl68 height=15 style='height:15.0pt'>
| + | |
- | <td height=15 class=xl68 width=426 style='height:15.0pt;width:426pt'> </td>
| + | |
- | <td class=xl69 width=63 style='border-left:none;width:63pt'> </td>
| + | |
- | <td class=xl68 width=34 style='border-left:none;width:34pt'> </td>
| + | |
- | <td class=xl68 width=64 style='border-left:none;width:64pt'> </td>
| + | |
- | <td class=xl68 width=64 style='border-left:none;width:64pt'> </td>
| + | |
- | <td class=xl68 width=77 style='border-left:none;width:77pt'> </td>
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- | </html>
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| + | See our [[Team:LMU-Munich/Data |Data page]] to see all our results. |
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| + | See our [[Team:LMU-Munich/Lab_Notebook/Protocols |Protocols Page]] to download a description of our methods. This is particular interesting if you want to start working with Bacillus subtilis as well. |
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