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Team:LMU-Munich/Lab Notebook
From 2012.igem.org
(Difference between revisions)
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{mso-style-parent:style0; | {mso-style-parent:style0; | ||
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border-top:.5pt solid windowtext; | border-top:.5pt solid windowtext; | ||
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border-bottom:1.0pt solid windowtext; | border-bottom:1.0pt solid windowtext; | ||
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white-space:normal;} | white-space:normal;} | ||
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{mso-style-parent:style0; | {mso-style-parent:style0; | ||
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text-align:center; | text-align:center; | ||
vertical-align:middle; | vertical-align:middle; | ||
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border-right:1.0pt solid windowtext; | border-right:1.0pt solid windowtext; | ||
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<tr height=15 style='height:15.0pt'> | <tr height=15 style='height:15.0pt'> | ||
<td height=15 class=xl65 width=426 style='height:15.0pt;width:426pt'>Accomplishment</td> | <td height=15 class=xl65 width=426 style='height:15.0pt;width:426pt'>Accomplishment</td> | ||
| - | <td class= | + | <td class=xl66 width=63 style='border-left:none;width:63pt'>Date</td> |
| - | <td class= | + | <td class=xl67 width=34 style='border-left:none;width:34pt'>Week</td> |
| - | <td class= | + | <td class=xl67 width=64 style='border-left:none;width:64pt'>Project</td> |
| - | <td class= | + | <td class=xl67 width=64 style='border-left:none;width:64pt'>Your name</td> |
| - | <td class= | + | <td class=xl72 width=77 style='border-left:none;width:77pt'>Data</td> |
</tr> | </tr> | ||
| - | <tr class= | + | <tr class=xl68 height=29 style='height:29.0pt'> |
| - | <td height= | + | <td height=29 class=xl70 width=426 style='height:29.0pt;width:426pt'>E.g. |
Created double-mutant using resistance-cassette KOs:<font class="font6"> cwlD</font><font | Created double-mutant using resistance-cassette KOs:<font class="font6"> cwlD</font><font | ||
class="font0">::kan+</font><font class="font6">gerD</font><font class="font0">::cat</font></td> | class="font0">::kan+</font><font class="font6">gerD</font><font class="font0">::cat</font></td> | ||
<td class=xl71 width=63 style='border-left:none;width:63pt'>8.8.2012</td> | <td class=xl71 width=63 style='border-left:none;width:63pt'>8.8.2012</td> | ||
| - | <td class= | + | <td class=xl70 width=34 style='border-left:none;width:34pt'>32</td> |
| - | <td class= | + | <td class=xl70 width=64 style='border-left:none;width:64pt'>Germination<font |
class="font5"><span style="mso-spacerun:yes"> </span>Stop</font></td> | class="font5"><span style="mso-spacerun:yes"> </span>Stop</font></td> | ||
| - | <td class= | + | <td class=xl70 width=64 style='border-left:none;width:64pt'>Jenny & Jara</td> |
| - | <td class= | + | <td class=xl70 width=77 style='border-left:none;width:77pt'> </td> |
</tr> | </tr> | ||
| - | <tr class= | + | <tr class=xl68 height=29 style='height:29.0pt'> |
| - | <td height= | + | <td height=29 class=xl68 width=426 style='height:29.0pt;width:426pt'>First |
| - | + | Single mutant: sleB::MLS</td> | |
| - | <td class= | + | <td class=xl69 width=63 style='border-left:none;width:63pt'>4.2.2012</td> |
| - | <td class= | + | <td class=xl68 width=34 style='border-left:none;width:34pt'>14</td> |
| - | <td class= | + | <td class=xl68 width=64 style='border-left:none;width:64pt'>Germination<font |
class="font5"><span style="mso-spacerun:yes"> </span>Stop</font></td> | class="font5"><span style="mso-spacerun:yes"> </span>Stop</font></td> | ||
| - | <td class= | + | <td class=xl68 width=64 style='border-left:none;width:64pt'>Jara</td> |
| - | <td class= | + | <td class=xl68 width=77 style='border-left:none;width:77pt'> </td> |
</tr> | </tr> | ||
| - | <tr class= | + | <tr class=xl68 height=29 style='height:29.0pt'> |
| - | <td height= | + | <td height=29 class=xl68 width=426 style='height:29.0pt;width:426pt'>Single |
| - | + | mutant: cwlJ::spec</td> | |
| - | <td class= | + | <td class=xl69 width=63 style='border-left:none;width:63pt'>4.2.2012</td> |
| - | <td class= | + | <td class=xl68 width=34 style='border-left:none;width:34pt'>14</td> |
| - | <td class= | + | <td class=xl68 width=64 style='border-left:none;width:64pt'>Germination<font |
class="font5"><span style="mso-spacerun:yes"> </span>Stop</font></td> | class="font5"><span style="mso-spacerun:yes"> </span>Stop</font></td> | ||
| - | <td class= | + | <td class=xl68 width=64 style='border-left:none;width:64pt'>Jara</td> |
| - | <td class= | + | <td class=xl68 width=77 style='border-left:none;width:77pt'> </td> |
</tr> | </tr> | ||
| - | <tr class= | + | <tr class=xl68 height=29 style='height:29.0pt'> |
| - | <td height= | + | <td height=29 class=xl68 width=426 style='height:29.0pt;width:426pt'>Single |
| - | + | mutant: gerD::cat</td> | |
| - | <td class= | + | <td class=xl69 width=63 style='border-left:none;width:63pt'>4.4.2012</td> |
| - | <td class= | + | <td class=xl68 width=34 style='border-left:none;width:34pt'>14</td> |
| - | <td class= | + | <td class=xl68 width=64 style='border-left:none;width:64pt'>Germination<font |
class="font5"><span style="mso-spacerun:yes"> </span>Stop</font></td> | class="font5"><span style="mso-spacerun:yes"> </span>Stop</font></td> | ||
| - | <td class= | + | <td class=xl68 width=64 style='border-left:none;width:64pt'>Jara</td> |
| - | <td class= | + | <td class=xl68 width=77 style='border-left:none;width:77pt'> </td> |
</tr> | </tr> | ||
| - | <tr class= | + | <tr class=xl68 height=29 style='height:29.0pt'> |
| - | <td height= | + | <td height=29 class=xl68 width=426 style='height:29.0pt;width:426pt'>First |
| - | + | Bacillus iGEM backbone! pSBBS0K-Pspac (from: pDG148-RFP)</td> | |
| - | <td class= | + | <td class=xl69 width=63 style='border-left:none;width:63pt'>4.5.2012</td> |
| - | <td class= | + | <td class=xl68 width=34 style='border-left:none;width:34pt'>14</td> |
| - | <td class= | + | <td class=xl68 width=64 style='border-left:none;width:64pt'>Bacillus BioBrick<font |
| - | + | class="font5">Box</font></td> | |
| - | <td class= | + | <td class=xl68 width=64 style='border-left:none;width:64pt'>Jara</td> |
| - | <td class= | + | <td class=xl68 width=77 style='border-left:none;width:77pt'> </td> |
</tr> | </tr> | ||
| - | <tr class= | + | <tr class=xl68 height=29 style='height:29.0pt'> |
| - | <td height= | + | <td height=29 class=xl68 width=426 style='height:29.0pt;width:426pt'>Single |
| - | + | mutant cwlD::kann</td> | |
| - | <td class= | + | <td class=xl69 width=63 style='border-left:none;width:63pt'>4.10.2012</td> |
| - | <td class= | + | <td class=xl68 width=34 style='border-left:none;width:34pt'>15</td> |
| - | <td class= | + | <td class=xl68 width=64 style='border-left:none;width:64pt'>Germination<font |
class="font5"><span style="mso-spacerun:yes"> </span>Stop</font></td> | class="font5"><span style="mso-spacerun:yes"> </span>Stop</font></td> | ||
| - | <td class= | + | <td class=xl68 width=64 style='border-left:none;width:64pt'>Jara</td> |
| - | <td class= | + | <td class=xl68 width=77 style='border-left:none;width:77pt'> </td> |
</tr> | </tr> | ||
| - | <tr class= | + | <tr class=xl68 height=15 style='height:15.0pt'> |
| - | <td height= | + | <td height=15 class=xl68 width=426 style='height:15.0pt;width:426pt'>GFP-spore |
| - | + | plasmids from Japan arrived</td> | |
| - | <td class= | + | <td class=xl69 width=63 style='border-left:none;width:63pt'>4.13.2012</td> |
| - | <td class= | + | <td class=xl68 width=34 style='border-left:none;width:34pt'>15</td> |
| - | <td class= | + | <td class=xl68 width=64 style='border-left:none;width:64pt'>Sporo<font |
class="font5">beads</font></td> | class="font5">beads</font></td> | ||
| - | <td class= | + | <td class=xl68 width=64 style='border-left:none;width:64pt'> </td> |
| - | <td class= | + | <td class=xl68 width=77 style='border-left:none;width:77pt'> </td> |
</tr> | </tr> | ||
| - | <tr class= | + | <tr class=xl68 height=29 style='height:29.0pt'> |
| - | <td height= | + | <td height=29 class=xl68 width=426 style='height:29.0pt;width:426pt'>Bacillus |
| - | + | pSBBS (pAH328-RFP)</td> | |
| - | <td class= | + | <td class=xl69 width=63 style='border-left:none;width:63pt'> </td> |
| - | <td class= | + | <td class=xl68 width=34 style='border-left:none;width:34pt'>0</td> |
| - | <td class= | + | <td class=xl68 width=64 style='border-left:none;width:64pt'>Bacillus BioBrick<font |
| - | + | class="font5">Box</font></td> | |
| - | <td class= | + | <td class=xl68 width=64 style='border-left:none;width:64pt'>Jara</td> |
| - | <td class= | + | <td class=xl68 width=77 style='border-left:none;width:77pt'> </td> |
</tr> | </tr> | ||
| - | <tr class= | + | <tr class=xl68 height=29 style='height:29.0pt'> |
| - | <td height= | + | <td height=29 class=xl68 width=426 style='height:29.0pt;width:426pt'>pSBBS1C-lacZ |
| - | + | (pAC6-RFP)</td> | |
| - | <td class= | + | <td class=xl69 width=63 style='border-left:none;width:63pt'>4.19.2012</td> |
| - | <td class= | + | <td class=xl68 width=34 style='border-left:none;width:34pt'>16</td> |
| - | <td class= | + | <td class=xl68 width=64 style='border-left:none;width:64pt'>Bacillus BioBrick<font |
| - | + | class="font5">Box</font></td> | |
| - | <td class= | + | <td class=xl68 width=64 style='border-left:none;width:64pt'>Jara</td> |
| - | <td class= | + | <td class=xl68 width=77 style='border-left:none;width:77pt'> </td> |
</tr> | </tr> | ||
| - | <tr class= | + | <tr class=xl68 height=29 style='height:29.0pt'> |
| - | <td height= | + | <td height=29 class=xl68 width=426 style='height:29.0pt;width:426pt'>pSBBS4S-Pxyl |
| - | + | (pXT-RFP)</td> | |
| - | <td class= | + | <td class=xl69 width=63 style='border-left:none;width:63pt'>7.9.2012</td> |
| - | <td class= | + | <td class=xl68 width=34 style='border-left:none;width:34pt'>28</td> |
| - | <td class= | + | <td class=xl68 width=64 style='border-left:none;width:64pt'>Bacillus BioBrick<font |
| - | + | class="font5">Box</font></td> | |
| - | <td class= | + | <td class=xl68 width=64 style='border-left:none;width:64pt'>Jara</td> |
| - | <td class= | + | <td class=xl68 width=77 style='border-left:none;width:77pt'> </td> |
</tr> | </tr> | ||
| - | <tr height= | + | <tr class=xl68 height=29 style='height:29.0pt'> |
| - | <td height= | + | <td height=29 class=xl68 width=426 style='height:29.0pt;width:426pt'>First |
| - | + | empty Bacillus backbone pSBBS1C (pDG1662-RFP)</td> | |
| - | <td class= | + | <td class=xl69 width=63 style='border-left:none;width:63pt'>7.9.2012</td> |
| - | <td class=xl68 style='border- | + | <td class=xl68 width=34 style='border-left:none;width:34pt'>28</td> |
| - | <td class= | + | <td class=xl68 width=64 style='border-left:none;width:64pt'>Bacillus BioBrick<font |
| - | + | class="font5">Box</font></td> | |
| - | <td class=xl68 style='border- | + | <td class=xl68 width=64 style='border-left:none;width:64pt'>Jara</td> |
| - | <td class=xl68 style='border- | + | <td class=xl68 width=77 style='border-left:none;width:77pt'> </td> |
</tr> | </tr> | ||
| - | <tr height= | + | <tr class=xl68 height=29 style='height:29.0pt'> |
| - | <td height= | + | <td height=29 class=xl68 width=426 style='height:29.0pt;width:426pt'>pSBBS4S |
(pDG1731-RFP)</td> | (pDG1731-RFP)</td> | ||
| - | <td class= | + | <td class=xl69 width=63 style='border-left:none;width:63pt'>7.9.2012</td> |
| - | <td class=xl68 style='border- | + | <td class=xl68 width=34 style='border-left:none;width:34pt'>28</td> |
| - | <td class= | + | <td class=xl68 width=64 style='border-left:none;width:64pt'>Bacillus BioBrick<font |
| - | + | class="font5">Box</font></td> | |
| - | <td class=xl68 style='border- | + | <td class=xl68 width=64 style='border-left:none;width:64pt'>Jara</td> |
| - | <td class=xl68 style='border- | + | <td class=xl68 width=77 style='border-left:none;width:77pt'> </td> |
</tr> | </tr> | ||
| - | <tr height= | + | <tr class=xl68 height=43 style='height:43.0pt'> |
| - | <td height= | + | <td height=43 class=xl68 width=426 style='height:43.0pt;width:426pt'>Double |
negative loop with lacZa finished (works qualitativly</td> | negative loop with lacZa finished (works qualitativly</td> | ||
| - | <td class= | + | <td class=xl69 width=63 style='border-left:none;width:63pt'>8.22.2012</td> |
| - | <td class=xl68 style='border- | + | <td class=xl68 width=34 style='border-left:none;width:34pt'>34</td> |
| - | <td class=xl68 style='border- | + | <td class=xl68 width=64 style='border-left:none;width:64pt'>double negative |
| - | <td class=xl68 style='border- | + | loop</td> |
| - | <td class=xl68 style='border- | + | <td class=xl68 width=64 style='border-left:none;width:64pt'>Julia</td> |
| + | <td class=xl68 width=77 style='border-left:none;width:77pt'> </td> | ||
| + | </tr> | ||
| + | <tr class=xl68 height=15 style='height:15.0pt'> | ||
| + | <td height=15 class=xl68 width=426 style='height:15.0pt;width:426pt'> </td> | ||
| + | <td class=xl69 width=63 style='border-left:none;width:63pt'> </td> | ||
| + | <td class=xl68 width=34 style='border-left:none;width:34pt'> </td> | ||
| + | <td class=xl68 width=64 style='border-left:none;width:64pt'> </td> | ||
| + | <td class=xl68 width=64 style='border-left:none;width:64pt'> </td> | ||
| + | <td class=xl68 width=77 style='border-left:none;width:77pt'> </td> | ||
| + | </tr> | ||
| + | <tr class=xl68 height=15 style='height:15.0pt'> | ||
| + | <td height=15 class=xl68 width=426 style='height:15.0pt;width:426pt'> </td> | ||
| + | <td class=xl69 width=63 style='border-left:none;width:63pt'> </td> | ||
| + | <td class=xl68 width=34 style='border-left:none;width:34pt'> </td> | ||
| + | <td class=xl68 width=64 style='border-left:none;width:64pt'> </td> | ||
| + | <td class=xl68 width=64 style='border-left:none;width:64pt'> </td> | ||
| + | <td class=xl68 width=77 style='border-left:none;width:77pt'> </td> | ||
| + | </tr> | ||
| + | <tr class=xl68 height=15 style='height:15.0pt'> | ||
| + | <td height=15 class=xl68 width=426 style='height:15.0pt;width:426pt'> </td> | ||
| + | <td class=xl69 width=63 style='border-left:none;width:63pt'> </td> | ||
| + | <td class=xl68 width=34 style='border-left:none;width:34pt'> </td> | ||
| + | <td class=xl68 width=64 style='border-left:none;width:64pt'> </td> | ||
| + | <td class=xl68 width=64 style='border-left:none;width:64pt'> </td> | ||
| + | <td class=xl68 width=77 style='border-left:none;width:77pt'> </td> | ||
| + | </tr> | ||
| + | <tr class=xl68 height=15 style='height:15.0pt'> | ||
| + | <td height=15 class=xl68 width=426 style='height:15.0pt;width:426pt'> </td> | ||
| + | <td class=xl69 width=63 style='border-left:none;width:63pt'> </td> | ||
| + | <td class=xl68 width=34 style='border-left:none;width:34pt'> </td> | ||
| + | <td class=xl68 width=64 style='border-left:none;width:64pt'> </td> | ||
| + | <td class=xl68 width=64 style='border-left:none;width:64pt'> </td> | ||
| + | <td class=xl68 width=77 style='border-left:none;width:77pt'> </td> | ||
| + | </tr> | ||
| + | <tr class=xl68 height=15 style='height:15.0pt'> | ||
| + | <td height=15 class=xl68 width=426 style='height:15.0pt;width:426pt'> </td> | ||
| + | <td class=xl69 width=63 style='border-left:none;width:63pt'> </td> | ||
| + | <td class=xl68 width=34 style='border-left:none;width:34pt'> </td> | ||
| + | <td class=xl68 width=64 style='border-left:none;width:64pt'> </td> | ||
| + | <td class=xl68 width=64 style='border-left:none;width:64pt'> </td> | ||
| + | <td class=xl68 width=77 style='border-left:none;width:77pt'> </td> | ||
| + | </tr> | ||
| + | <tr class=xl68 height=15 style='height:15.0pt'> | ||
| + | <td height=15 class=xl68 width=426 style='height:15.0pt;width:426pt'> </td> | ||
| + | <td class=xl69 width=63 style='border-left:none;width:63pt'> </td> | ||
| + | <td class=xl68 width=34 style='border-left:none;width:34pt'> </td> | ||
| + | <td class=xl68 width=64 style='border-left:none;width:64pt'> </td> | ||
| + | <td class=xl68 width=64 style='border-left:none;width:64pt'> </td> | ||
| + | <td class=xl68 width=77 style='border-left:none;width:77pt'> </td> | ||
| + | </tr> | ||
| + | <tr class=xl68 height=15 style='height:15.0pt'> | ||
| + | <td height=15 class=xl68 width=426 style='height:15.0pt;width:426pt'> </td> | ||
| + | <td class=xl69 width=63 style='border-left:none;width:63pt'> </td> | ||
| + | <td class=xl68 width=34 style='border-left:none;width:34pt'> </td> | ||
| + | <td class=xl68 width=64 style='border-left:none;width:64pt'> </td> | ||
| + | <td class=xl68 width=64 style='border-left:none;width:64pt'> </td> | ||
| + | <td class=xl68 width=77 style='border-left:none;width:77pt'> </td> | ||
| + | </tr> | ||
| + | <tr class=xl68 height=15 style='height:15.0pt'> | ||
| + | <td height=15 class=xl68 width=426 style='height:15.0pt;width:426pt'> </td> | ||
| + | <td class=xl69 width=63 style='border-left:none;width:63pt'> </td> | ||
| + | <td class=xl68 width=34 style='border-left:none;width:34pt'> </td> | ||
| + | <td class=xl68 width=64 style='border-left:none;width:64pt'> </td> | ||
| + | <td class=xl68 width=64 style='border-left:none;width:64pt'> </td> | ||
| + | <td class=xl68 width=77 style='border-left:none;width:77pt'> </td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
Revision as of 12:07, 23 August 2012
The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".
[ more news ]
Lab Notebook
| Accomplishment | Date | Week | Project | Your name | Data |
| E.g. Created double-mutant using resistance-cassette KOs: cwlD::kan+gerD::cat | 8.8.2012 | 32 | Germination Stop | Jenny & Jara | |
| First Single mutant: sleB::MLS | 4.2.2012 | 14 | Germination Stop | Jara | |
| Single mutant: cwlJ::spec | 4.2.2012 | 14 | Germination Stop | Jara | |
| Single mutant: gerD::cat | 4.4.2012 | 14 | Germination Stop | Jara | |
| First Bacillus iGEM backbone! pSBBS0K-Pspac (from: pDG148-RFP) | 4.5.2012 | 14 | Bacillus BioBrickBox | Jara | |
| Single mutant cwlD::kann | 4.10.2012 | 15 | Germination Stop | Jara | |
| GFP-spore plasmids from Japan arrived | 4.13.2012 | 15 | Sporobeads | ||
| Bacillus pSBBS (pAH328-RFP) | 0 | Bacillus BioBrickBox | Jara | ||
| pSBBS1C-lacZ (pAC6-RFP) | 4.19.2012 | 16 | Bacillus BioBrickBox | Jara | |
| pSBBS4S-Pxyl (pXT-RFP) | 7.9.2012 | 28 | Bacillus BioBrickBox | Jara | |
| First empty Bacillus backbone pSBBS1C (pDG1662-RFP) | 7.9.2012 | 28 | Bacillus BioBrickBox | Jara | |
| pSBBS4S (pDG1731-RFP) | 7.9.2012 | 28 | Bacillus BioBrickBox | Jara | |
| Double negative loop with lacZa finished (works qualitativly | 8.22.2012 | 34 | double negative loop | Julia | |
