Team:WashU/Week11
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We transformed <i>E. coli</i> GC5 cells with the ligation results from yesterday and plated the cells. | We transformed <i>E. coli</i> GC5 cells with the ligation results from yesterday and plated the cells. | ||
- | In addition, we created a standard curve for crocin and calculated the extinction coefficient from the data. The picture below shows different absorbances of crocin at various concentrations, and then, using a trendline, we calculated the extinction coefficient of crocin. Since the slope is equal to path length multiplied by extinction coefficient, taking 7629 and dividing it by the path length, 0.5 cm, gives an extinction coefficient of 14538 M | + | In addition, we created a standard curve for crocin and calculated the extinction coefficient from the data. The picture below shows different absorbances of crocin at various concentrations, and then, using a trendline, we calculated the extinction coefficient of crocin. Since the slope is equal to path length multiplied by extinction coefficient, taking 7629 and dividing it by the path length, 0.5 cm, gives an extinction coefficient of 14538 M<sup>-1</sup> cm<sup>-1</sup>. |
https://lh4.googleusercontent.com/-M8LiDs-XiX0/UC6UsmlPBaI/AAAAAAAAAiU/jQ0ebf-e-hw/s640/Extinction%2520Coefficient.png | https://lh4.googleusercontent.com/-M8LiDs-XiX0/UC6UsmlPBaI/AAAAAAAAAiU/jQ0ebf-e-hw/s640/Extinction%2520Coefficient.png | ||
Revision as of 19:04, 17 August 2012