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Team:LMU-Munich/Bacillus BioBricks
From 2012.igem.org
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==Bacillus BioBricks== | ==Bacillus BioBricks== | ||
| - | We will create a toolbox of Bacillus BioBricks to contribute to the registry. | + | We will create a toolbox of <i>Bacillus</i> BioBricks to contribute to the registry. |
'''We would like to introduce ''Bacillus'' to the world of iGEM!!!''' | '''We would like to introduce ''Bacillus'' to the world of iGEM!!!''' | ||
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Here is a list of all the vectors we cloned and used. Please note that pMAD is not in a BioBrick standard, but it is a useful tool to knock out genes inBacillus subtilis. | Here is a list of all the vectors we cloned and used. Please note that pMAD is not in a BioBrick standard, but it is a useful tool to knock out genes inBacillus subtilis. | ||
| - | For the use of our vectors, please see our [Team:LMU-Munich/Lab_Notebook/Protocols Protocols] page. A general introduction to Bacillus subtilis and its integrative vectors can be found [[Team:LMU-Munich/Bacillus Introduction|here]]. | + | For the use of our vectors, please see our [Team:LMU-Munich/Lab_Notebook/Protocols Protocols] page. A general introduction to Bacillus subtilis and its integrative vectors can be found [[Team:LMU-Munich/Bacillus Introduction|here]]. All vectors have ampicillin as <i>E. coli</i> resistance and RFP as selection marker. |
| - | {- | + | {| |
| + | |- | ||
|name | |name | ||
|E. coli res. | |E. coli res. | ||
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|? | |? | ||
|- | |- | ||
| - | + | |pSB<sub>Bs</sub>4S | |
| - | } | + | |Amp |
| + | |Spec | ||
| + | |thrC | ||
| + | |empty | ||
| + | |pDG1731 | ||
| + | |? | ||
| + | |- | ||
| + | |pSB<sub>Bs</sub>1C | ||
| + | |Amp | ||
| + | |Cam | ||
| + | |amyE | ||
| + | |empty | ||
| + | |pDG1662 | ||
| + | |? | ||
| + | |- | ||
| + | |pSB<sub>Bs</sub>4S-P<sub><i>Xyl</i></sub> | ||
| + | |Amp | ||
| + | |Spec | ||
| + | |thrC | ||
| + | |with Xylose-inducible promoter | ||
| + | |pXT | ||
| + | |? | ||
| + | |- | ||
| + | |pSB<sub>Bs</sub>3C-<i>luxABCDE</i> | ||
| + | |Amp | ||
| + | |Cam | ||
| + | |sacA | ||
| + | |with <i>luxABCDE</i> reporter cassette | ||
| + | |pAH328 | ||
| + | |? | ||
| + | |- | ||
| + | |pSB<sub>Bs</sub>0K-P<sub><i>spac</i></sub> | ||
| + | |Amp | ||
| + | |Kan | ||
| + | |replicative | ||
| + | |with IPTG inducible promoter | ||
| + | |pDG148 | ||
| + | |? | ||
| + | |- | ||
| + | |pSB<sub>Bs</sub>2E | ||
| + | |Amp | ||
| + | |MLS | ||
| + | |lacA | ||
| + | |empty | ||
| + | |pAX01 | ||
| + | |? | ||
| + | |- | ||
| + | |} | ||
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{{:Team:LMU-Munich/Templates/Page Footer}} | {{:Team:LMU-Munich/Templates/Page Footer}} | ||
Revision as of 16:42, 15 August 2012
The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".
[ more news ]
Bacillus BioBricks
We will create a toolbox of Bacillus BioBricks to contribute to the registry.
We would like to introduce Bacillus to the world of iGEM!!!
This Bacillus BioBrick Box contains compatible vectors and useful promoters.
Here is a list of all the vectors we cloned and used. Please note that pMAD is not in a BioBrick standard, but it is a useful tool to knock out genes inBacillus subtilis.
For the use of our vectors, please see our [Team:LMU-Munich/Lab_Notebook/Protocols Protocols] page. A general introduction to Bacillus subtilis and its integrative vectors can be found here. All vectors have ampicillin as E. coli resistance and RFP as selection marker.
| name | E. coli res. | B. subt. res. | insertion locus | description | derived from | reference |
| pSBBs1C-lacZ | Amp | Cam | amyE | with lacZ reporter gene | pAC6 | ? |
| pSBBs4S | Amp | Spec | thrC | empty | pDG1731 | ? |
| pSBBs1C | Amp | Cam | amyE | empty | pDG1662 | ? |
| pSBBs4S-PXyl | Amp | Spec | thrC | with Xylose-inducible promoter | pXT | ? |
| pSBBs3C-luxABCDE | Amp | Cam | sacA | with luxABCDE reporter cassette | pAH328 | ? |
| pSBBs0K-Pspac | Amp | Kan | replicative | with IPTG inducible promoter | pDG148 | ? |
| pSBBs2E | Amp | MLS | lacA | empty | pAX01 | ? |
