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Team:Cambridge/Project/FluorideRiboswitch
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We also plan on implementing and characterising a Fluoride riboswitch. This, unlike the Magnesium construct, is a positive regulator. The riboswitch, originally isolated from Bacillus cereus, serves as a transcriptional attenuator in the abscence of fluoride. In the presence of fluoride its conformation changes and the repression is lifted. In B. cereus this serves to permit translation of a fluoride efflux pump, which allows the bacteria to cope with the, potentially toxic, elevated fluoride levels in which it finds itself. | We also plan on implementing and characterising a Fluoride riboswitch. This, unlike the Magnesium construct, is a positive regulator. The riboswitch, originally isolated from Bacillus cereus, serves as a transcriptional attenuator in the abscence of fluoride. In the presence of fluoride its conformation changes and the repression is lifted. In B. cereus this serves to permit translation of a fluoride efflux pump, which allows the bacteria to cope with the, potentially toxic, elevated fluoride levels in which it finds itself. | ||
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| + | The construct we hope to make is shown below: | ||
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| + | [[F-riboswitch.gif|700px|center|thumb|Our riboswitch construct for the detection of fluoride.]] | ||
===References=== | ===References=== | ||
Revision as of 13:12, 15 August 2012
Fluoride riboswitch
We also plan on implementing and characterising a Fluoride riboswitch. This, unlike the Magnesium construct, is a positive regulator. The riboswitch, originally isolated from Bacillus cereus, serves as a transcriptional attenuator in the abscence of fluoride. In the presence of fluoride its conformation changes and the repression is lifted. In B. cereus this serves to permit translation of a fluoride efflux pump, which allows the bacteria to cope with the, potentially toxic, elevated fluoride levels in which it finds itself.
The construct we hope to make is shown below:
700px|center|thumb|Our riboswitch construct for the detection of fluoride.
References
- Jenny L. Baker et al., Widespread Genetic Switches and Toxicity Resistance Proteins for Fluoride, Science 335, 233-235.
