Protocol: TAE
To make a new 200 ml of %0x TAE :
- Tris base : 78.4 g
- Acetic Acid (100 %) : 11.4 ml
- EDTA : 20ml 0.5 M
- 292.25M --> 146.12 = 0.5 mol --> 1.46 g in 10 ml is 0.5M
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To make a new 200 ml of %0x TAE :