Team:WashU/Week12

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We also PCR'd the Z construct in the submission plasmid, Psb1c3. We then digested the results with both EcoRI and PstI, as shown below, and saw that the PCR had indeed succeded.<br>
We also PCR'd the Z construct in the submission plasmid, Psb1c3. We then digested the results with both EcoRI and PstI, as shown below, and saw that the PCR had indeed succeded.<br>
https://lh6.googleusercontent.com/-7oeDkDT2Ef8/UClEy74B65I/AAAAAAAAAgI/_8myIyOxHdI/s800/zeeparts.jpg
https://lh6.googleusercontent.com/-7oeDkDT2Ef8/UClEy74B65I/AAAAAAAAAgI/_8myIyOxHdI/s800/zeeparts.jpg
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Finally, we ran an SDS-PAGE gel of our Z construct with a histidine tag in order to see the proteins produced using an extraction protocol from Sigma found here [INSERT PROTOCOL].
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Revision as of 23:33, 13 August 2012



Monday, August 13

Crocin Extraction

To accurately verify that our construct is indeed producing zeaxanthin, spectrophotometry must be used to characterize our biobrick part. To this end, we tested crocin in two different solvents, along with our Z^2 construct, compared to the absorption spectrum of zeaxanthin in hexane.

http://dl.dropbox.com/u/88390549/CrocinGraphs.png

This indicates that our Z^2 construct isn't actually producing crocin, or if it is, the crocin isn't expressed highly enough to absorb significantly.

Saffron in a Kan

We also PCR'd the Z construct in the submission plasmid, Psb1c3. We then digested the results with both EcoRI and PstI, as shown below, and saw that the PCR had indeed succeded.
zeeparts.jpg

Finally, we ran an SDS-PAGE gel of our Z construct with a histidine tag in order to see the proteins produced using an extraction protocol from Sigma found here [INSERT PROTOCOL].
Tuesday, August 14



Wednesday, August 15



Thursday, August 16



Friday, August 17

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