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Team:UC Chile/Cyano/Labook/week9
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| + | <p><font face="Calibri"><font size="5">April 30 - May 6</font></font></p> | ||
| + | <br> | ||
| + | <p><font face="Calibri"><font size="4">Monday</font></font></p> | ||
| + | <br> | ||
| + | <p><font face="Calibri"> | ||
| + | *Electrophoresis for constructs C1.1 and C1.2 assembled through Gibson. Wrong size. | ||
| + | *PCR run of parts C1.1 and C1.2 for new Gibson. | ||
| + | </font></p> | ||
| + | <br> | ||
| + | <p><font face="Calibri"><font size="4">Tuesday</font></font></p> | ||
| + | <br> | ||
| + | <p><font face="Calibri"> | ||
| + | *KanR + double terminator has strange results. An analytic digestion will be done to check insertion. | ||
| + | *As Gibson assembly is not working, we still need to try if pPMQAK1 works in synechocystis. We'll take out the toxic by standard assembly and transform Synechocystis afterwards. | ||
| + | *Negative control of competent cells with pPMQAK1 was positive. Possible contamination. | ||
| + | *There are colonies in Gibson negative controls. | ||
| + | </font></p> | ||
| + | <br> | ||
| + | <p><font face="Calibri"><font size="4">Wednesday</font></font></p> | ||
| + | <br> | ||
| + | <p><font face="Calibri"> | ||
| + | *Gibson assembly for C1.1, C 1/2 and positive control.</font></p> | ||
| + | <br> | ||
| + | <p><font face="Calibri"><font size="4">Friday</font></font></p> | ||
| + | <br> | ||
| + | <p><font face = "Calibri"> | ||
| + | *Colony PCR for C1 and C1/2. | ||
| + | *New competent cells prepared. | ||
| + | </font></p> | ||
Latest revision as of 21:37, 13 August 2012
April 30 - May 6
Monday
Tuesday
Wednesday
Friday
