Team:UC Chile/Cyano/Labook/week3

From 2012.igem.org

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<p><font face="Calibri"><font size= "5">March 19 - March 25</font></font></p>
<p><font face="Calibri"><font size= "5">March 19 - March 25</font></font></p>
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<p><font face="Calibri"><font size="4">Thursday</font></font></p>
<p><font face="Calibri"><font size="4">Thursday</font></font></p>
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* Expression plasmid pPMQAK1 (received from Sweden in February) was left growing.
* Expression plasmid pPMQAK1 (received from Sweden in February) was left growing.
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* A designed construct was wrong. Included a template that was not in the kit 2011 (KanR + Double terminator, part K292003)
* A designed construct was wrong. Included a template that was not in the kit 2011 (KanR + Double terminator, part K292003)
* Part K292003 was built by standard assembly (B1003+B1004)
* Part K292003 was built by standard assembly (B1003+B1004)
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<p><font face="Calibri"><font size="4">Sunday</font></font></p>
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*Biobrick assembly protocol according to Registry Protocol. Assembly: B1003+B1004 in pSB1C3.</font></p>

Latest revision as of 19:41, 13 August 2012

Cyanolux & Bactomithril - Pontificia Universidad Católica de Chile, iGEM 2012


March 19 - March 25


Thursday


  • Expression plasmid pPMQAK1 (received from Sweden in February) was left growing.
  • Brick J33210 was transformed in a broad host plasmid.
  • New batch of competent E. coli (E. cloni) was prepared.
  • Mipreps pPMQAK1 and pSB4K5
  • Primers in for C1 and others (primers designed in meeting 1)
  • Synechocystis PCC6803 culture was examined under confocal microscopy. The culture was not axenic.
  • A designed construct was wrong. Included a template that was not in the kit 2011 (KanR + Double terminator, part K292003)
  • Part K292003 was built by standard assembly (B1003+B1004)


Sunday


  • Biobrick assembly protocol according to Registry Protocol. Assembly: B1003+B1004 in pSB1C3.

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