Team:WashU/Week12

From 2012.igem.org

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This indicates that our Z^2 construct isn't actually producing crocin, or if it is, the crocin isn't expressed highly enough to absorb significantly.
This indicates that our Z^2 construct isn't actually producing crocin, or if it is, the crocin isn't expressed highly enough to absorb significantly.
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<h1>Saffron in a Kan</h1>
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We also PCR'd the Z construct with the TOPO vector. We then digested the results with both EcoRI and PstI, as shown below, and saw that the PCR had indeed succeded.<br>
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https://lh6.googleusercontent.com/-aW9tqbYATtI/UCkwzWiT3_I/AAAAAAAAAfc/bnp_C29c-ms/s800/zeeparts.jpg
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Revision as of 16:54, 13 August 2012



Monday, August 13

Crocin Extraction

To accurately verify that our construct is indeed producing zeaxanthin, spectrophotometry must be used to characterize our biobrick part. To this end, we tested crocin in two different solvents, along with our Z^2 construct, compared to the absorption spectrum of zeaxanthin in hexane.

http://dl.dropbox.com/u/88390549/CrocinGraphs.png

This indicates that our Z^2 construct isn't actually producing crocin, or if it is, the crocin isn't expressed highly enough to absorb significantly.

Saffron in a Kan

We also PCR'd the Z construct with the TOPO vector. We then digested the results with both EcoRI and PstI, as shown below, and saw that the PCR had indeed succeded.
zeeparts.jpg
Tuesday, August 14



Wednesday, August 15



Thursday, August 16



Friday, August 17

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