"
Team:KAIT Japan/Notebook
From 2012.igem.org
(Difference between revisions)
| Line 15: | Line 15: | ||
During the creation<br> | During the creation<br> | ||
| - | + | ==8/9== | |
| - | + | ||
| - | + | ==8/11== | |
| - | + | ===The purified DNA1,2=== | |
#Electrophoresis | #Electrophoresis | ||
#*Marker:pigment(buffer) 1μL,DNA molecule 2μL,TE buffer 3μL | #*Marker:pigment(buffer) 1μL,DNA molecule 2μL,TE buffer 3μL | ||
| Line 25: | Line 24: | ||
#*Gel concentration:1.2%,Migration time:30minutes | #*Gel concentration:1.2%,Migration time:30minutes | ||
#Storage | #Storage | ||
| - | + | ===PCR Product 1,3,9=== | |
#Electrophoresis | #Electrophoresis | ||
#*The gel check and cut | #*The gel check and cut | ||
Revision as of 02:19, 13 August 2012
| Home | Team | Project | Parts | Attributions | Notebook | Safety | Sitemap |
|---|
During the creation
Contents |
8/9
8/11
The purified DNA1,2
- Electrophoresis
- Marker:pigment(buffer) 1μL,DNA molecule 2μL,TE buffer 3μL
- Sample:pigment(buffer) 1μL,sample 5μL
- Gel concentration:1.2%,Migration time:30minutes
- Storage
PCR Product 1,3,9
- Electrophoresis
- The gel check and cut
- DNA purification
- Confirmation of electrophoresis
- PCR
- 50cycle
- Storage
