"

From 2012.igem.org

Revision as of 11:49, 12 August 2012 (view source) Nili (Talk | contribs) ← Older edit		Revision as of 02:19, 13 August 2012 (view source) Nili (Talk | contribs) Newer edit →
Line 14:		Line 14:
	During the creation<br>		During the creation<br>
-	You should make use of the calendar feature on the wiki and start a lab notebook.  This may be looked at by the judges to see how your work progressed throughout the summer.  It is a very useful organizational tool as well.	+
		+	==Notebook==
		+	===8/9===
		+
		+	===8/11===
		+	====The purified DNA1,2====
		+	#Electrophoresis
		+	#*Marker:pigment(buffer) 1μL,DNA molecule 2μL,TE buffer 3μL
		+	#*Sample:pigment(buffer) 1μL,sample 5μL
		+	#*Gel concentration:1.2%,Migration time:30minutes
		+	#Storage
		+	====PCR Product 1,3,9====
		+	#Electrophoresis
		+	#*The gel check and cut
		+	#DNA purification
		+	#Confirmation of electrophoresis
		+	#PCR
		+	#*50cycle
		+	#Storage
		+	----

---

Revision as of 02:19, 13 August 2012

Home	Team	Project	Parts	Attributions	Notebook	Safety	Sitemap

During the creation

Contents 1 Notebook 1.1 8/9 1.2 8/11 1.2.1 The purified DNA1,2 1.2.2 PCR Product 1,3,9

Notebook

8/9

8/11

The purified DNA1,2

1. Electrophoresis
   • Marker:pigment(buffer) 1μL,DNA molecule 2μL,TE buffer 3μL
   • Sample:pigment(buffer) 1μL,sample 5μL
   • Gel concentration:1.2%,Migration time:30minutes
2. Storage

PCR Product 1,3,9

1. Electrophoresis
   • The gel check and cut
2. DNA purification
3. Confirmation of electrophoresis
4. PCR
   • 50cycle
5. Storage

---