Team:WashU/Protocols/PCR

From 2012.igem.org

(Difference between revisions)
 
(5 intermediate revisions not shown)
Line 1: Line 1:
-
{{WashUback}}
+
__NOTOC__
-
 
+
<!--Thanks to UW iGEM '11 for the above instructions.  The original source can be found here: https://2011.igem.org/Team:Washington/Protocols/PCR-->
 +
{{WashUbackprotocols}}
 +
<div id = "protocolshort">
=General PCR Protocol=
=General PCR Protocol=
Line 16: Line 18:
#40 cycles 94 degrees (1 minute), 50 degrees (1 minute), 72 degrees (1 minute)
#40 cycles 94 degrees (1 minute), 50 degrees (1 minute), 72 degrees (1 minute)
-
==Source==
+
<div align="center">
-
Thanks to UW iGEM '11 for the above instructions.  The original source can be found here: [https://2011.igem.org/Team:Washington/Protocols/gel_electrophoresis University Washington gel electrophoresis protocol]
+
<font size ="5">
 +
[https://2012.igem.org/Team:WashU/Protocols Back to Protocols]

Latest revision as of 21:30, 9 August 2012


General PCR Protocol

Set up PCR reaction tube

  1. 1uL Template
  2. 1uL 25mM dNTP's
  3. 10uL Buffer
  4. 0.5uL Forward Primer
  5. 0.5uL Reverse Primer
  6. 0.5uL TAQ polymerase
  7. 36.5uL diH2O

Amplification reaction in thermocycler

  1. 1 cycle 94 degrees (3 minutes)
  2. 40 cycles 94 degrees (1 minute), 50 degrees (1 minute), 72 degrees (1 minute)
Retrieved from "http://2012.igem.org/Team:WashU/Protocols/PCR"