"
Team:UC Chile/Cyano/Notepad/week23
From 2012.igem.org
(Difference between revisions)
| Line 5: | Line 5: | ||
Also, transformation of E. coli with Lux Brick took place to try our franky's chick sensor. | Also, transformation of E. coli with Lux Brick took place to try our franky's chick sensor. | ||
| - | We did a PCR colony of the bacteria with C1.1, C1.2, Pcaa3 and ADF3. | + | We did a PCR colony of the bacteria with C1.1, C1.2, Pcaa3 and ADF3 and finished with a regular PCR for our constructs parts. |
Oh, and Pollak is really sick but comes to the lab anyway... | Oh, and Pollak is really sick but comes to the lab anyway... | ||
| Line 14: | Line 14: | ||
WE HAVE CONSTRUCT 1.1 READY! After the selection by colony PCR of constructs assembled last wednesday, we digested them and colony number 8 of C1.1 is the right size. YEAH! The rest of colony PCR's although (wednesday's C1.2 and the friday assemblies) were... well, bad results. | WE HAVE CONSTRUCT 1.1 READY! After the selection by colony PCR of constructs assembled last wednesday, we digested them and colony number 8 of C1.1 is the right size. YEAH! The rest of colony PCR's although (wednesday's C1.2 and the friday assemblies) were... well, bad results. | ||
| - | Oh, and we discovered our beautiful syne that was growing in the lab downstairs seems to be cough cough dead cough cough. They die with glucose... We reinoculated our stock for a new transformation and left coli growing with the target construct (psbAB + GFP). | + | Oh, and we discovered our beautiful syne that was growing in the lab downstairs seems to be cough cough dead cough cough. They die with glucose... We reinoculated our stock for a new transformation and left coli growing with the target construct (psbAB + GFP). |
| + | |||
| + | Finally did a PCR run to amplify parts for constructs C1.2, Pcaa3 and ADF3 and several Lux parts. | ||
Wednesday: Today was arduino day finally. We could try the system sensitivity to bacterial light and turns out franky's chick (the light sensor) can measure intensity pretty well. Also, a short experiment was done. We prepared several flasks with e. coli with Lux brick in sob medium and we added different concentrations of glucose to the different flasks (prior arabinose induction). The objective was to find the optimum concentration of glucose able to produce biolumniscence. You can check the details of the experiment in the Results section. | Wednesday: Today was arduino day finally. We could try the system sensitivity to bacterial light and turns out franky's chick (the light sensor) can measure intensity pretty well. Also, a short experiment was done. We prepared several flasks with e. coli with Lux brick in sob medium and we added different concentrations of glucose to the different flasks (prior arabinose induction). The objective was to find the optimum concentration of glucose able to produce biolumniscence. You can check the details of the experiment in the Results section. | ||
Revision as of 19:45, 9 August 2012
