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Team:Toronto/Project
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| + | Wiki Under Construction <br> <br> | ||
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| + | project description | ||
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| + | This year’s project is <b>two-fold</b>, and it involves engineering <i>Arabidopsis thaliana</i> with two constructs <br> | ||
| + | that would be important <b>proof of concepts</b> for further studies of feasibility in crops. Furthermore, the <br> | ||
| + | project will address the pressing need of iGEM-compatible <b>BioBricks specific to plants </b>, which would <br> | ||
| + | allow for an increased focus on important agricultural research for iGEM teams worldwide. <br> | ||
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| + | <h3> part I </h3> | ||
| + | The availability of soil phosphorous is considered globally to be a limiting factor of productivity for <br> | ||
| + | a significant majority of agricultural land. Phosphorus-based fertilizers are used to avoid this <br> | ||
| + | deficiency. Most of the fertilizer, however, often becomes fixed into inorganic and organic fractions, <br> | ||
| + | and consequently is unavailable to plant roots. Moreover, according to Environment Canada, phosphorus <br> | ||
| + | enrichment and runoff into surface waters “continues to be a <b>national issue </b>” .The first construct of <br> | ||
| + | this project would allow for <b>extracellular <i>secretion</i> of phytase</b> from Arabidopsis roots allowing the <br> | ||
| + | plants to utilize the accumulated forms of soil organic phosphorus (primarily, phytate), which <br> | ||
| + | otherwise would not be available to the plant. <br> | ||
| + | <br> | ||
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| + | <h3> part II </h3> | ||
| + | With the sole exception of low elevation equatorial ecosystems, plants anywhere else are often subject to <br> | ||
| + | temperatures that are often lower than their threshold for <b>growth and survival</b>. The second construct for <br> | ||
| + | this project aims to increase the range of tolerance to <b>low temperature stress</b> in Arabidoposis by <br> | ||
| + | incorporating an antifreeze protein in the plant. This builds on Yale’s project last year. <br> | ||
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Revision as of 01:42, 8 August 2012
Wiki Under Construction
project description
This year’s project is two-fold, and it involves engineering Arabidopsis thaliana with two constructs
that would be important proof of concepts for further studies of feasibility in crops. Furthermore, the
project will address the pressing need of iGEM-compatible BioBricks specific to plants , which would
allow for an increased focus on important agricultural research for iGEM teams worldwide.
part I
The availability of soil phosphorous is considered globally to be a limiting factor of productivity fora significant majority of agricultural land. Phosphorus-based fertilizers are used to avoid this
deficiency. Most of the fertilizer, however, often becomes fixed into inorganic and organic fractions,
and consequently is unavailable to plant roots. Moreover, according to Environment Canada, phosphorus
enrichment and runoff into surface waters “continues to be a national issue ” .The first construct of
this project would allow for extracellular secretion of phytase from Arabidopsis roots allowing the
plants to utilize the accumulated forms of soil organic phosphorus (primarily, phytate), which
otherwise would not be available to the plant.
part II
With the sole exception of low elevation equatorial ecosystems, plants anywhere else are often subject totemperatures that are often lower than their threshold for growth and survival. The second construct for
this project aims to increase the range of tolerance to low temperature stress in Arabidoposis by
incorporating an antifreeze protein in the plant. This builds on Yale’s project last year.
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