Team:Evry/Notebook/w9

From 2012.igem.org

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<h2>Monday, 6th August</h2>
<h2>Monday, 6th August</h2>
 +
<h3>Reporter test</h3>
 +
We had a lot of reporters BB but we didn't know if they're all working. We test them with a electrophoresis:
 +
Two RFP, a GFP, a CFP and a YFP worked.
 +
 +
<h3>GFP BB</h3>
 +
Digestion of GFP BB with EcoRI and PstI, gel extraction and DNA purification.
 +
Goal: insertion in pCS2+ BB
 +
Issue: Not enough DNA for ligation.
<h2>Tuesday, 7th August</h2>
<h2>Tuesday, 7th August</h2>

Revision as of 11:47, 7 August 2012

Weeks:

June July August September October November

Week 9: 6th August - 12th August

Monday, 6th August

Reporter test

We had a lot of reporters BB but we didn't know if they're all working. We test them with a electrophoresis: Two RFP, a GFP, a CFP and a YFP worked.

GFP BB

Digestion of GFP BB with EcoRI and PstI, gel extraction and DNA purification. Goal: insertion in pCS2+ BB Issue: Not enough DNA for ligation.

Tuesday, 7th August