Team:WashU/Week11

From 2012.igem.org

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Revision as of 20:54, 6 August 2012

Monday, August 6

Colony PCR of U construct using four new colonies and the same positive Z control has once again resulted in no successful colonies. However, the PCR has reaffirmed that the control works, which is a good sign.

We also did a PCR of the color constructs in order to form our BioPaint set. [PICTURE BELOW]

Since our T plasmid and Z construct ligations have not been going well, we started over by preparing new cultures of Z and T, running them on a gel, and then gel purifying the results. [SEE PICTURE]

We began 5 ml cultures for the good Z construct with TOPO and for one of the U ligation colonies. We will use the Z culture for a glycerol stock, and digest the U culture to see if our ligations actually succeded.

Tuesday, August 7

Wednesday, August 8

Thursday, August 9

Friday, August 10

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+Colony PCR of U construct using four new colonies and the same positive Z control has once again resulted in no successful colonies. However, the PCR has reaffirmed that the control works, which is a good sign.
+We also did a PCR of the color constructs in order to form our BioPaint set. [PICTURE BELOW]
+Since our T plasmid and Z construct ligations have not been going well, we started over by preparing new cultures of Z and T, running them on a gel, and then gel purifying the results. [SEE PICTURE]
+We began 5 ml cultures for the good Z construct with TOPO and for one of the U ligation colonies. We will use the Z culture for a glycerol stock, and digest the U culture to see if our ligations actually succeded.
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