From 2012.igem.org

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Contents 1 Introduction: Motivation 2 Primary Objective: A Useful BioBrick for Synthetic Biologists 3 Secondary Objective: Secondary Objective 4 Further Considerations

Introduction: Motivation

• We seek to develop a BioBrick that will allow researchers in the field of synthetic biology to accurately measure translational efficiency, and transcriptional strength.

• We believe that we can use Spinach as a biosensor to reflect these metrics in vivo, rather than in vitro, which has previously proven to be very costly and impractical.

• We will characterize the relationship between genetic expression of Spinach (upstream), translational efficiency, and transcriptional strength.
  

Place abstract/info here

Motivation question?

Humanistic implications go here

Primary Objective: A Useful BioBrick for Synthetic Biologists

We have been xxxxxyyyy for the purpose of xxxyyy which is detailed by the following:

1. something great
2. something greater
3. something awesome

This will be xxxyyy

Secondary Objective: Secondary Objective

We have also been continuing the work of our 2012 team in engineering Escherichia coli to be awesome. Here is a link to awesomeness (WHO).

Further Considerations

In the pursuit of our project, as well as the biological aspects, we:

• considered aspects of scale-up, including the ethical, legal and social implications of our potential final product, MicroMaize,
• programmed a new piece of software for use in metabolic modelling,
• developed and tested a number of new techniques to make the process of creating BioBricks^TM that little bit easier,
• collaborated with the University of Guelph team, sharing carotenoid synthesis genes, flux modulators, and gram positive plasmids.

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