Team:Carnegie Mellon

From 2012.igem.org

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<li class="toclevel-1"><a href="#Introduction:_Motivation"><span class="tocnumber">1</span> <span class="toctext">Introduction: Motivation</span></a></li>
<li class="toclevel-1"><a href="#Introduction:_Motivation"><span class="tocnumber">1</span> <span class="toctext">Introduction: Motivation</span></a></li>
<li class="toclevel-1"><a href="#Primary_Objective:_A_Useful_BioBrick_for_Synthetic_Biologists"><span class="tocnumber">2</span> <span class="toctext">Primary Objective: A Useful BioBrick for Synthetic Biologists</span></a></li>
<li class="toclevel-1"><a href="#Primary_Objective:_A_Useful_BioBrick_for_Synthetic_Biologists"><span class="tocnumber">2</span> <span class="toctext">Primary Objective: A Useful BioBrick for Synthetic Biologists</span></a></li>
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<li class="toclevel-1"><a href="#Secondary_Objective:_Solving_vitamin_A_deficiency_in_the_developing_world"><span class="tocnumber">3</span> <span class="toctext">Secondary Objective: Solving vitamin A deficiency in the developing world</span></a></li>
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<li class="toclevel-1"><a href="#Secondary_Objective:_Secondary_Objective"><span class="tocnumber">3</span> <span class="toctext">Secondary Objective: Solving vitamin A deficiency in the developing world</span></a></li>
<li class="toclevel-1"><a href="#Further_Considerations"><span class="tocnumber">4</span> <span class="toctext">Further Considerations</span></a></li>
<li class="toclevel-1"><a href="#Further_Considerations"><span class="tocnumber">4</span> <span class="toctext">Further Considerations</span></a></li>
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<a name="Primary_Objective:_A_Useful_BioBrick_for_Synthetic_Biologists"></a><h2> <span class="mw-headline"> Primary Objective: A Useful BioBrick for Synthetic Biologists </span></h2>
<a name="Primary_Objective:_A_Useful_BioBrick_for_Synthetic_Biologists"></a><h2> <span class="mw-headline"> Primary Objective: A Useful BioBrick for Synthetic Biologists </span></h2>
<div class="thumb tright"><div class="thumbinner" style="width:152px;"><a href="/Image:Carnegie_Mellon-MicroMaize.jpg" class="image" title="A false-colour SEM of MicroMaize cells."><img alt="A false-colour SEM of MicroMaize cells." src="/wiki/images/thumb/0/08/Carnegie_Mellon-MicroMaize.jpg/150px-Carnegie_Mellon-MicroMaize.jpg" width="150" height="301" border="0" class="thumbimage" /></a>  <div class="thumbcaption"><div class="magnify"><a href="/Image:Carnegie_Mellon-MicroMaize.jpg" class="internal" title="Enlarge"><img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="" /></a></div>A false-colour SEM of MicroMaize cells.</div></div></div>
<div class="thumb tright"><div class="thumbinner" style="width:152px;"><a href="/Image:Carnegie_Mellon-MicroMaize.jpg" class="image" title="A false-colour SEM of MicroMaize cells."><img alt="A false-colour SEM of MicroMaize cells." src="/wiki/images/thumb/0/08/Carnegie_Mellon-MicroMaize.jpg/150px-Carnegie_Mellon-MicroMaize.jpg" width="150" height="301" border="0" class="thumbimage" /></a>  <div class="thumbcaption"><div class="magnify"><a href="/Image:Carnegie_Mellon-MicroMaize.jpg" class="internal" title="Enlarge"><img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="" /></a></div>A false-colour SEM of MicroMaize cells.</div></div></div>
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<p>We have been investigating engineering bacteria to <b>produce starch from the cellulose in waste biomass</b> (that is agricultural waste, wood chippings, waste from paper production etc.). This starch could be:
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<p>We have been xxxxxyyyy <b>for the purpose of xxxyyy </b> Detailed by the following:
</p>
</p>
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<ol><li> sold to the biofuels industry for conversion to ethanol
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<ol><li> something great
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</li><li> used as feed for livestock
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</li><li> something greater
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</li><li> used as a starch supplement in the human diet if needed.
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</li><li> something awesome
</li></ol>
</li></ol>
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<p>This will reduce our dependence on fossil fuels and free up agricultural land for the growth of food crops, ultimately putting less strain on ecosystems.
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<p>This will be xxxyyy
</p>
</p>
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<a name="Secondary_Objective:_Solving_vitamin_A_deficiency_in_the_developing_world"></a><h2> <span class="mw-headline"> Secondary Objective: Solving vitamin A deficiency in the developing world </span></h2>
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<a name="Secondary_Objective:_Secondary_Objective"></a><h2> <span class="mw-headline"> Secondary Objective: Solving vitamin A deficiency in the developing world </span></h2>
<p>We have also been continuing the work of <a href="https://2007.igem.org/Carnegie_Mellon/Yoghurt" class="external text" title="https://2007.igem.org/Carnegie_Mellon/Yoghurt" rel="nofollow">our 2007 team</a> in engineering <i>Escherichia coli</i> to <b>produce the vitamin A precursor β-carotene</b>. Vitamin A is required for vision and a healthy immune system. 250,000-500,000 children in the developing world lose their vision each year, half of them dying within 12 months of this (<a href="http://www.who.int/nutrition/topics/vad/en/" class="external text" title="http://www.who.int/nutrition/topics/vad/en/" rel="nofollow">WHO</a>).
<p>We have also been continuing the work of <a href="https://2007.igem.org/Carnegie_Mellon/Yoghurt" class="external text" title="https://2007.igem.org/Carnegie_Mellon/Yoghurt" rel="nofollow">our 2007 team</a> in engineering <i>Escherichia coli</i> to <b>produce the vitamin A precursor β-carotene</b>. Vitamin A is required for vision and a healthy immune system. 250,000-500,000 children in the developing world lose their vision each year, half of them dying within 12 months of this (<a href="http://www.who.int/nutrition/topics/vad/en/" class="external text" title="http://www.who.int/nutrition/topics/vad/en/" rel="nofollow">WHO</a>).
</p>
</p>

Revision as of 19:05, 22 May 2012

Team:Carnegie_Mellon - 20012.igem.org

Team:Carnegie_Mellon

From 2012.igem.org

Contents

Introduction: Motivation

  • We seek to develop a BioBrick that will allow researchers in the field of synthetic biology to accurately measure translational efficiency, and transcriptional strength.
  • We believe that we can use Spinach as a biosensor to reflect these metrics in vivo, rather than in vitro, which has previously proven to be very costly and impractical.
  • We will characterize the relationship between genetic expression of Spinach (upstream), translational efficiency, and transcriptional strength.


Place abstract/info here

Motivation question?

Humanistic implications go here

Primary Objective: A Useful BioBrick for Synthetic Biologists

A false-colour SEM of MicroMaize cells.
A false-colour SEM of MicroMaize cells.

We have been xxxxxyyyy for the purpose of xxxyyy Detailed by the following:

  1. something great
  2. something greater
  3. something awesome

This will be xxxyyy

Secondary Objective: Solving vitamin A deficiency in the developing world

We have also been continuing the work of our 2007 team in engineering Escherichia coli to produce the vitamin A precursor β-carotene. Vitamin A is required for vision and a healthy immune system. 250,000-500,000 children in the developing world lose their vision each year, half of them dying within 12 months of this (WHO).

Further Considerations

In the pursuit of our project, as well as the biological aspects, we:

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