Team:WashU/Protocols/PCR

From 2012.igem.org

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<!--Thanks to UW iGEM '11 for the above instructions.  The original source can be found here: https://2011.igem.org/Team:Washington/Protocols/PCR-->
<!--Thanks to UW iGEM '11 for the above instructions.  The original source can be found here: https://2011.igem.org/Team:Washington/Protocols/PCR-->
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=General PCR Protocol=
=General PCR Protocol=

Revision as of 19:30, 2 August 2012


General PCR Protocol

Set up PCR reaction tube

  1. 1uL Template
  2. 1uL 25mM dNTP's
  3. 10uL Buffer
  4. 0.5uL Forward Primer
  5. 0.5uL Reverse Primer
  6. 0.5uL TAQ polymerase
  7. 36.5uL diH2O

Amplification reaction in thermocycler

  1. 1 cycle 94 degrees (3 minutes)
  2. 40 cycles 94 degrees (1 minute), 50 degrees (1 minute), 72 degrees (1 minute)
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