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Team:Leicester/August2012
From 2012.igem.org
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<p>(9:30 am) Several members of the team are given wrong start times, so only 1 student arrived in the lab at 9:30, however with the work needed to be done all the rest of the team are quickly on their way.</p> | <p>(9:30 am) Several members of the team are given wrong start times, so only 1 student arrived in the lab at 9:30, however with the work needed to be done all the rest of the team are quickly on their way.</p> | ||
<p>(10:00 am) Today the team is going to start creating a DNA library of the ''Pseudomonas. sp'' so that once the NB26 strain arrives it can be compared straight away to give an idea which genes could be involved in the degredation of polystyrene and to help start narrowing our search.</p> | <p>(10:00 am) Today the team is going to start creating a DNA library of the ''Pseudomonas. sp'' so that once the NB26 strain arrives it can be compared straight away to give an idea which genes could be involved in the degredation of polystyrene and to help start narrowing our search.</p> | ||
| - | <p> To create the library a few things need to be known: Is the bacteria gram positive or negative? Is the DNA chromosomal or plasmid? How much DNA is going to be extracted? All ''Pseudomonas'' is gram negative, and the team wants to extract as much of the | + | <p> To create the library a few things need to be known: Is the bacteria gram positive or negative? Is the DNA chromosomal or plasmid? How much DNA is going to be extracted? All ''Pseudomonas'' is gram negative, and the team wants to extract as much of the plasmid DNA as possible, as this is where the team thinks the polystyrene degredation enzyme is located. With all these answered the group can collect the right DNA extraction kit and start working.</p> |
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Revision as of 09:11, 1 August 2012
Wednesday 1st August 2012
(9:30 am) Several members of the team are given wrong start times, so only 1 student arrived in the lab at 9:30, however with the work needed to be done all the rest of the team are quickly on their way.
(10:00 am) Today the team is going to start creating a DNA library of the ''Pseudomonas. sp'' so that once the NB26 strain arrives it can be compared straight away to give an idea which genes could be involved in the degredation of polystyrene and to help start narrowing our search.
To create the library a few things need to be known: Is the bacteria gram positive or negative? Is the DNA chromosomal or plasmid? How much DNA is going to be extracted? All ''Pseudomonas'' is gram negative, and the team wants to extract as much of the plasmid DNA as possible, as this is where the team thinks the polystyrene degredation enzyme is located. With all these answered the group can collect the right DNA extraction kit and start working.
