Team:EPF-Lausanne/Protocol/PreparePlasmidExtraction

(Difference between revisions)

Revision as of 13:38, 30 July 2012

Select and number colonies on the plates.
Prepare tubes of LB medium with the correct quantity of antibiotics (100 µg/ml for Amp and Spc).
- Amp can be found in the -20ºC freezer at Ecoli, labeled as "stock". It is 100 µg/µl, or 1000x.
- The tubes to be used are the 14 ml round bottom found in front of the iGEM drawers. Culture with cap in the first step (loose) and close to the second step after culture.
Touch each colony with a clean pipette tip and put it in a tube.
Put in incubator.

@@ Line 9: / Line 9: @@
 * Select and number colonies on the plates.
-* Prepare tubes of LB medium with the correct quantity of antibiotics (100ug/ml for Amp and Spc).
+* Prepare tubes of LB medium with the correct quantity of antibiotics (100 µg/ml for Amp and Spc).
+** Amp can be found in the -20ºC freezer at Ecoli, labeled as "stock". It is 100 µg/µl, or 1000x.
+** The tubes to be used are the 14 ml round bottom found in front of the iGEM drawers. Culture with cap in the first step (loose) and close to the second step after culture.
 * Touch each colony with a clean pipette tip and put it in a tube.
 * Put in incubator.