Team:XMU-China/CSS/SpryMenuBarHorizontal/css
From 2012.igem.org
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- | < | + | <meta http-equiv="Content-Type" |
- | + | content="text/html; charset=utf-8" /> | |
- | + | <title>无标题文档</title> | |
- | + | <script | |
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- | + | src="SpryAssets/SpryMenuBar.js" type="text/javascript"></script> | |
- | + | <link | |
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- | + | href="SpryAssets/SpryMenuBarHorizontal.css" rel="stylesheet" type="text/css" /> | |
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- | + | <style | |
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- | # | + | type="text/css"> |
- | position: | + | #schoolbadge { |
- | + | position:absolute; | |
- | + | width:50px; | |
- | + | height:50px; | |
+ | z- | ||
- | + | index:4; | |
- | + | left:100px; | |
- | + | top:3px; | |
- | + | padding-left:20px; | |
- | + | font-size:30px; | |
- | + | ||
- | + | ||
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- | + | ||
- | + | color: #006dab; | |
- | + | font-family:Tahoma, Geneva, sans-serif; | |
- | + | text-align:center; | |
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} | } | ||
+ | #leftnavig { | ||
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- | + | position:absolute; | |
- | + | width:145px; | |
- | + | height:115px; | |
- | + | z-index:2; | |
- | + | left: 100px; | |
- | + | top: | |
+ | |||
+ | 55px; | ||
} | } | ||
- | + | #titlebar { | |
- | + | font-size: 13px; | |
- | + | color: #006DAB; | |
- | + | background-image: url | |
- | + | ||
- | + | (pictures/index.png); | |
- | + | background-repeat: no-repeat; | |
- | + | position: absolute; | |
- | + | z-index: 1; | |
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- | + | width: 900px; | |
- | + | left: 245px; | |
- | + | top: 55px; | |
- | + | display: table; | |
- | + | letter-spacing: 0px; | |
} | } | ||
+ | #photo { | ||
+ | |||
- | + | position:absolute; | |
- | + | width:840px; | |
- | + | height:100px; | |
- | + | z-index:3; | |
- | + | left:245px; | |
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- | + | top:80px; | |
- | + | visibility:inherit; | |
- | + | padding:30px; | |
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} | } | ||
+ | #description { | ||
+ | position: absolute; | ||
+ | |||
- | + | width:840px; | |
- | + | left: 245px; | |
- | + | top: 210px; | |
- | + | margin:30px; | |
- | + | table-layout:fixed; | |
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+ | height:350px; | ||
+ | overflow: scroll; | ||
+ | } | ||
</style> | </style> | ||
+ | |||
</head> | </head> | ||
<body> | <body> | ||
- | + | <div id="schoolbadge"><img | |
- | + | ||
- | <div id=" | + | |
- | + | ||
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- | + | src="https://static.igem.org/mediawiki/2012/6/67/Schoolbadge.gif" width="100%" /></div> | |
- | + | <div | |
- | + | ||
- | + | ||
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- | + | id="leftnavig"><table border="0" cellspacing="0" cellpadding="0" | |
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- | + | background="https://static.igem.org/mediawiki/2012/a/a2/Left-navigbar.png" width="100%"> | |
- | + | <tr> | |
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- | </ | + | <td height="22"> Project description</td> |
+ | </tr> | ||
+ | |||
- | < | + | <tr> |
- | + | <td height="22"> Main results</td> | |
- | + | </tr> | |
- | + | ||
- | + | ||
- | + | <tr> | |
- | + | <td height="22"> Data</td> | |
- | + | </tr> | |
- | + | <tr> | |
- | + | ||
- | + | ||
- | + | <td height="22"> Protocals</td> | |
- | + | </tr> | |
- | + | <tr> | |
- | + | <td | |
- | + | ||
- | + | height="22"> Team members</td> | |
- | + | </tr> | |
- | + | <tr> | |
- | + | ||
- | + | ||
- | + | <td><img src="https://static.igem.org/mediawiki/2012/a/a5/Leftbar.png" alt="pictures/leftbar.png" | |
- | + | ||
- | + | width="145" /></td> | |
- | + | </tr> | |
- | + | </table></div> | |
- | + | <ul id="titlebar" class="MenuBarHorizontal" | |
- | + | ||
- | + | style="background:url(pictures/navigbar.png) ;"> | |
- | + | <li><a | |
- | + | ||
- | </ | + | href="#https://2012.igem.org/Team:XMU-China">Home</a></li> |
+ | <li><a class="MenuBarItemSubmenu" | ||
+ | |||
+ | href="#" >Team</a> | ||
+ | <ul> | ||
+ | <li><a href="#">Instructors</a></li> | ||
+ | <li><a | ||
+ | |||
+ | href="#">Advisors</a></li> | ||
+ | <li><a href="#">Team members</a></li> | ||
+ | <li><a | ||
+ | |||
+ | href="#">Acknowledgement</a></li> | ||
+ | <li><a href="#">Collaborations</a></li> | ||
+ | <li><a | ||
+ | |||
+ | href="#">Our city&Our university</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li><a | ||
+ | |||
+ | class="MenuBarItemSubmenu" href="#" >Project</a> | ||
+ | <ul> | ||
+ | <li><a href="#">Background | ||
+ | |||
+ | |||
+ | (Approach/ | ||
+ | Program)</a></li> | ||
+ | <li><a href="#">Section A</a></li> | ||
+ | <li><a | ||
+ | |||
+ | href="#">Section B</a></li> | ||
+ | <li><a href="#">Accomplishment</a></li> | ||
+ | <li><a | ||
+ | |||
+ | href="#">Data page</a></li> | ||
+ | <li><a href="#">Future plan</a></li> | ||
+ | <li><a | ||
+ | |||
+ | href="#">Reference</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li><a class="MenuBarItemSubmenu" | ||
+ | |||
+ | href="#">Parts</a> | ||
+ | <ul> | ||
+ | <li><a href="#">Parts submitted</a></li> | ||
+ | <li><a | ||
+ | |||
+ | href="#">Attrivutions</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li><a class="MenuBarItemSubmenu" | ||
+ | |||
+ | href="#">Notebook</a> | ||
+ | <ul> | ||
+ | <li><a href="#">Brainstorm</a></li> | ||
+ | <li><a | ||
+ | |||
+ | href="#">Wet lab journal</a></li> | ||
+ | <li><a href="#">Protocols</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | |||
+ | |||
+ | <li><a href="#">Safety</a></li> | ||
+ | <li><a class="MenuBarItemSubmenu" href="#">HumanPractice</a> | ||
+ | |||
+ | |||
+ | <ul> | ||
+ | <li><a href="#">Exhibition</a></li> | ||
+ | <li><a href="#">Communication</a></li> | ||
+ | |||
+ | |||
+ | </ul> | ||
+ | <li><a href="#https://2012.igem.org">iGEM</a></li> | ||
+ | </li> | ||
+ | </ul> | ||
+ | |||
+ | <!--<div | ||
+ | |||
+ | id="photo"><img src="" alt="放图~" name="用来放图~" width="100%" height="100%" id="photo2" | ||
+ | |||
+ | /></div> --> | ||
+ | |||
+ | <div id="description"><table width="100%" border="0" cellspacing="0" | ||
+ | |||
+ | cellpadding="0" > | ||
+ | <tr> | ||
+ | <p>Description</p> | ||
+ | <hr /> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><img | ||
+ | |||
+ | src="https://static.igem.org/mediawiki/2012/c/cd/Fig2.png" width="108" height="168" /></td> | ||
+ | |||
+ | |||
+ | <td><p> The aim of this study is to construct a fluorescent digital | ||
+ | |||
+ | display device of genetic circuits with synthetic logic gates. The finished device will | ||
+ | |||
+ | display and switch numbers. Similar to electronic circuits, logic regulation operation in | ||
+ | |||
+ | cells integrates the extracellular and intracellular signals. We assemble several pairs of | ||
+ | |||
+ | promoters and their activators (or repressors) into computing building block of the circuit: | ||
+ | |||
+ | <I>P<font size="1px">BAD</font></I>-Arabinose,<I>P<font size="1px">cI</font></I>-CI, and | ||
+ | |||
+ | <I>P<font size="1px">tetR</font></I>-TetR. These computation units act as genetic logic gates | ||
+ | |||
+ | perform AND, OR and NOT gates function. In order to light up our digital numbers, we put | ||
+ | |||
+ | Green Fluorescent Protein (GFP) in the downstream expression, which is ubiquitous in the | ||
+ | |||
+ | field of biological signal protein. Considering that the common types of GFP usually tend to | ||
+ | |||
+ | be very stable and hard to "quench", we choose unstable GFP to make our device reusable and | ||
+ | |||
+ | convert in a fast speed. This unstable GFP is tagged with a C-terminal extension, which will | ||
+ | |||
+ | be recognized and degraded by tail-specific proteases, leading to a short half-life and fast | ||
+ | |||
+ | degradation of the protein. Degradation rate should be confined at a suitable range, | ||
+ | |||
+ | otherwise the device would either have no light or take a long time to change numbers. | ||
+ | |||
+ | </a><br /> | ||
+ | |||
+ | Moreover, we intend to develop a unique | ||
+ | |||
+ | display device in such a way that each unit lights up one after another. To meet the | ||
+ | |||
+ | challenge, we set out to engineer a series of cells with variable time-delayed expression | ||
+ | |||
+ | characters under the inspiration of Feed Forward Loops (FFLs) in transcription network. As | ||
+ | |||
+ | shown in Figure 1, both X and Y are transcriptional activators of coherent type 1 FFL with | ||
+ | |||
+ | "AND-gate" function. When signal Sx appears, X becomes active and stimulates the downstream | ||
+ | |||
+ | promoters, which are capable of inducing the production of Y and Z. Due to the "AND gate" | ||
+ | |||
+ | function, Z starts to accumulate only when Y yields at a threshold. By controlling the Y | ||
+ | |||
+ | production and the activation threshold for the <I>Z</I> promoter, we can alter the duration | ||
+ | |||
+ | of time-delay in the expression of GFP. Lux system will still play an important role based on | ||
+ | |||
+ | our project last year. Promoter <I>luxpR</I> with various modifications in threshold will be | ||
+ | |||
+ | created by site-directed mutagenesis. | ||
+ | </a><br /> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </tr> | ||
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</div> | </div> | ||
+ | <script | ||
- | + | type="text/javascript"> | |
- | + | var MenuBar1 = new Spry.Widget.MenuBar("titlebar", | |
- | + | ||
- | + | {imgDown:"SpryAssets/SpryMenuBarDownHover.gif", | |
+ | imgRight:"SpryAssets/SpryMenuBarRightHover.gif"}); | ||
+ | </script> | ||
+ | </body> | ||
</html> | </html> |
Revision as of 09:21, 30 July 2012
- REDIRECT Template:Team:XMU-China/head
The aim of this study is to construct a fluorescent digital
display device of genetic circuits with synthetic logic gates. The finished device will
display and switch numbers. Similar to electronic circuits, logic regulation operation in
cells integrates the extracellular and intracellular signals. We assemble several pairs of
promoters and their activators (or repressors) into computing building block of the circuit:
PBAD-Arabinose,PcI-CI, and
PtetR-TetR. These computation units act as genetic logic gates
perform AND, OR and NOT gates function. In order to light up our digital numbers, we put
Green Fluorescent Protein (GFP) in the downstream expression, which is ubiquitous in the
field of biological signal protein. Considering that the common types of GFP usually tend to
be very stable and hard to "quench", we choose unstable GFP to make our device reusable and
convert in a fast speed. This unstable GFP is tagged with a C-terminal extension, which will
be recognized and degraded by tail-specific proteases, leading to a short half-life and fast
degradation of the protein. Degradation rate should be confined at a suitable range,
otherwise the device would either have no light or take a long time to change numbers.
|