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Team:St Andrews/Procedure
From 2012.igem.org
(Difference between revisions)
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<dt>date and gene</dt> | <dt>date and gene</dt> | ||
<dd>result</dd> | <dd>result</dd> | ||
| - | + | ||
| - | <dd> | + | <dt>29/6/12 ELO7 L. major</dt> |
| - | <dt>23/7/12 Δ12 Syn. | + | <dd><p><ul>After showing successful insertion of <i>L. major</i> Δ6 elongase in pET-15b, the sample was sent off for sequencing. Unfortunately, the gene sequenced was not the Δ6 elongase we were hoping to find, but a <i>T. brucei</i> mevalonate kinase, which was previously inserted in the pET-15b vectors we had been using. Thus, we digested some fresh pET-15b to be used in the future.</ul></p> |
| + | |||
| + | <p><ul><a data-toggle="modal" href="#modal6" class="btn btn-primary btn-mini">Sequence</a> | ||
| + | <div class="modal hide" id="modal6"> | ||
| + | <div class="modal-header"> | ||
| + | <button type="button" class="close" data-dismiss="modal">×</button> | ||
| + | <h3>ELO6 in pET-15b</h3> | ||
| + | </div> | ||
| + | <div class="modal-body"> | ||
| + | <p>GnanTTTGTTtAcTttaagaaggagaTATACCATGGGcAGCAGCCATcatCATcatcaTCACAGCAGCGGCCTggtGCCG | ||
| + | CGCGGCagCCATATGGTCGTGGCATCGtgTCCGGGAAAAGTGCTGATTCTTGGTGGTTAcCTAATAGttGAGGAGCCAAA | ||
| + | TGTTGGTATTTCCGTAGGAACAACCGCGCGCTTCGTGACACGTGTGGCGTCgtgGAAAAAATGCTCTGATGGAAAGTGTC | ||
| + | GAGTGCACATCGTCTCGTCACAGTTCAACAAggaGTTTACGTTTGAATGCGCGGCTGAAGAGGACAGTGATTCCACTATC | ||
| + | AAGATTGTGCAATTAGAGGGTGCACCATCGCCGTTTCTCTTCTACGGTATTTTGTACTCGGTTGCCGGCGCTCTATTATT | ||
| + | TGGTGGTGATATTTTTCGTGATGTTACCCTAGAGTTGTTGGCGGATAACGATTTCTACAGCCAAAGGAATTATTTGGAGT | ||
| + | CCCAAGGCAAGCCGGTGACCGCAGCCAACCTGCGGCTCATCCCCCGTTATACGCCCCTCTTGGGGGAGGTTTCCAAAACT | ||
| + | GGTCTAGGATCTTCAGCGGCAATGACCACTAGTGTCGTAGCGTGCCTGCTCCAACTTTTTGTTTTTGATTCGAAGAAAAA | ||
| + | CAATGCCACTGAGTCCGTGGAGAGGGCACCGGAGCTACCTCTACGTTTGGAGGATGTAACAGAGTTCATTCATCGTATTA | ||
| + | GTCAGGTTGCCCACTGCGTAGCACAGGGAAAAGTGgGGAGCGGGTTTGATGTCTACACGGCCACGTTCGGGACGTGTGTG | ||
| + | TACCGACGGTTCTCCGCACGCGTGCTGGAnAAGCTCGTAAAGGgtAATGAACCTCCGAAGCGCGTCGCTATTCCTCTTTT | ||
| + | ACGTGAGTGTGTCGAGACGGATGAAGTGTGgGTTCAGAGGATACcCTtccGGCTTCCTACCGGGTTGCAGCTTCTTTTAG | ||
| + | GGGATGTACACAAGGGAGgAacaGAAAcACCTGGCATGGTGTcgaAGGTCATGTCatgnnagnggTCGGTGACCacGGAC | ||
| + | CCcAACAGnttgnggnnnngantTcCCATgnntAAT | ||
| + | </p> | ||
| + | </div> | ||
| + | <div class="modal-footer"> | ||
| + | <a href="#" class="btn" data-dismiss="modal">Close</a> | ||
| + | </div> | ||
| + | </div></ul> | ||
| + | </ul></p></dd> | ||
| + | |||
| + | |||
| + | <dt>23/7/12 Δ12 Syn.</dt> | ||
<dd><p><ul>15b forward gave 0 nucleotides</ul></p> | <dd><p><ul>15b forward gave 0 nucleotides</ul></p> | ||
<p><ul>15b reverse gave 0 nucleotides</ul></p> | <p><ul>15b reverse gave 0 nucleotides</ul></p> | ||
Revision as of 15:14, 27 July 2012
Lab Book
Procedure
- date
- what we did
- date
- what we did
- date
- what we did
anyone really keen to write this up, up to protein visualization? Don't forget to mention the differing restriction enzymes and vectors of each team. I can add in links to the protocols page.
Please also refer to our Protocols page.
Sequences Primers Sequencing results Lipid analysis
Start from lipid extraction
primers, sequence results
Sequences
who knows what those Metal Mickies are up to. Eating nachos in the Whey Pat most likely. MEGA LOLS (all caps, with a Z).
PrimersDigestionTransformation
Primers
All primers are notated 5' to 3'.
