Revision as of 14:42, 27 July 2012

Lab Book

Procedure

date: what we did
date: what we did
date: what we did

anyone really keen to write this up, up to protein visualization? Don't forget to mention the differing restriction enzymes and vectors of each team. I can add in links to the protocols page.

Please also refer to our Protocols page.

Sequences Primers Sequencing results Lipid analysis

Start from lipid extraction

primers, sequence results

Sequences

Lipid analysis

who knows what those Metal Mickies are up to. Eating nachos in the Whey Pat most likely. MEGA LOLS (all caps, with a Z).

Primers

All primers are notated 5' to 3'.

For primer annealing in the PCR, the primer sequences were combined in the following way:

GST Forward and Ni Reverse

GST Forward and Ni₂ Reverse

GST Forward and Pd Reverse

GST Forward and Pt Reverse

@@ Line 535: / Line 535: @@
 <p>For primer annealing in the PCR, the primer sequences were combined in the following way:</p>
-<li>GST Forward and Ni Reverse</li>
+<li><strong>GST Forward</strong> and <strong>Ni Reverse</strong></li>
-<li>GST Forward + Ni<sub>2</sub> Reverse</li>
+<li><strong>GST Forward</strong> and <strong>Ni<sub>2</sub> Reverse</strong></li>
-<li>GST Forward + Pd Reverse</li>
+<li><strong>GST Forward</strong> and <strong>Pd Reverse</strong></li>
-<li>GST Forward + Pt Reverse</li>
+<li><strong>GST Forward</strong> and <strong>Pt Reverse</strong></li>
 <BR>&nbsp;</BR>

Team:St Andrews/Procedure

From 2012.igem.org

Revision as of 14:42, 27 July 2012

Δ12 T. Cruzi

Δ12 Synechocystis

Δ15 Synechocystis

Δ6 Synechocystis

ELO 6 L. major