Team:WashU/Week9

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Ordered Primers
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Ligation of RFP construct with PSL2131
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Revision as of 20:55, 25 July 2012



Monday, July 23

Today we sonicated three cultures of E. coli - one with our Z construct, one with Z, C and inducers, and one with Z, C, but no inducer. Then, we extracted the soluble proteins from those cultures and ran them on a gel. [PICTURE]

We also ran a PCR of a series of ligations. The color constructs were ligated with promoters into chloramphenicol-resistant plasmid Psb1C3. [EXPAND UPON THIS]

Extracted proteins from cultures and took spectrophotometric readings - show graphs


Tuesday, July 24

Ordered Primers

Ligation of RFP construct with PSL2131



Wednesday, July 25


Thursday, July 26


Friday, July 27


Friday, July 28

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