Team:St Andrews/Procedure

From 2012.igem.org

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     atgtcgcgggttgacaatttaactgtcgcgccgggtccaccggatgtcatgaaggcggtg
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     <div class="span5"><p><font size="1">atgtcgcgggttgacaatttaactgtcgcgccgggtccaccggatgtcatgaaggcggtg
cttaagtctgggcgcaacaaggagcacaatgtcattgtgccttcctccgcactgaccatt
cttaagtctgggcgcaacaaggagcacaatgtcattgtgccttcctccgcactgaccatt
ggagaaattcaggagaaaatcccggtaaagtactttgaacgcaacactacccggagcgtc
ggagaaattcaggagaaaatcccggtaaagtactttgaacgcaacactacccggagcgtc
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cactacaacgcaatccaggtgacaagaaagtacattcgtgagatactgggtgccacatac
cactacaacgcaatccaggtgacaagaaagtacattcgtgagatactgggtgccacatac
attacggatgagaggtcactgtggaagatgctctgggaacagcgtagagagtgccgctat
attacggatgagaggtcactgtggaagatgctctgggaacagcgtagagagtgccgctat
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gttgttcccgcagagggcgtctgtgtctttcatgggtaa
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gttgttcccgcagagggcgtctgtgtctttcatgggtaa</font></p></div>
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   atgactgccacgattcccccgttgacaccaacggtaacgcccagcaatcccgatcgcccg
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   <div class="span5"><p><font size="1">atgactgccacgattcccccgttgacaccaacggtaacgcccagcaatcccgatcgcccg
attgcggatctcaaactacaagacatcattaaaaccctgcccaaggaatgcttcgagaaa
attgcggatctcaaactacaagacatcattaaaaccctgcccaaggaatgcttcgagaaa
aaagcgagcaaagcctgggcttctgttttgattaccctaggggcgatcgccgtgggctat
aaagcgagcaaagcctgggcttctgttttgattaccctaggggcgatcgccgtgggctat
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ctacgactagcccacggaagtttaaaagaaaactggggaccttttctttacgagcgcacc
ctacgactagcccacggaagtttaaaagaaaactggggaccttttctttacgagcgcacc
tttaactggcaattaatgcaacaaattagtgggcaatgtcatttatatgaccccgaacat
tttaactggcaattaatgcaacaaattagtgggcaatgtcatttatatgaccccgaacat
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ggctaccgcaccttcggctccctgaaaaaagtttaa
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ggctaccgcaccttcggctccctgaaaaaagtttaa</p></div>
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   gtgcgtctagaaatttcatcgcctcaaacaaagcttccttaccccaaaactgaagaatta
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   <div class="span5"><p><font size="1">gtgcgtctagaaatttcatcgcctcaaacaaagcttccttaccccaaaactgaagaatta
ccatttaccctccaagagctcagaaacgctattccagcggattgttttgagccatcggta
ccatttaccctccaagagctcagaaacgctattccagcggattgttttgagccatcggta
gtccggtccttgggctacttttttttggatgttggtttaattgccgggttttatgctcta
gtccggtccttgggctacttttttttggatgttggtttaattgccgggttttatgctcta
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aagttacgccgggcgactgaagccatcaagcccattttaggggaatattatcgatattct
aagttacgccgggcgactgaagccatcaagcccattttaggggaatattatcgatattct
gacgagccaatttggcaagctttttttaagtcctactgggcttgccattttgttcctaat
gacgagccaatttggcaagctttttttaagtcctactgggcttgccattttgttcctaat
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caaggttcaggggtctattaccaatccccatccaatggtggatatcaaaagaaaccttaa
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caaggttcaggggtctattaccaatccccatccaatggtggatatcaaaagaaaccttaa</p></div>
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   atgctaacagcggaaagaattaaatttacccagaaacgggggtttcgtcgggtactaaac
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   <div class="span5"><p><font size="1">atgctaacagcggaaagaattaaatttacccagaaacgggggtttcgtcgggtactaaac
caacgggtggatgcctactttgccgagcatggcctgacccaaagggataatccctccatg
caacgggtggatgcctactttgccgagcatggcctgacccaaagggataatccctccatg
tatctgaaaaccctgattattgtgctctggttgttttccgcttgggcctttgtgcttttt
tatctgaaaaccctgattattgtgctctggttgttttccgcttgggcctttgtgcttttt
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aatcaccaagttacccaccatcttttccccaatatttgtcatattcactatccccaattg
aatcaccaagttacccaccatcttttccccaatatttgtcatattcactatccccaattg
gaaaatattattaaggatgtttgccaagagtttggtgtggaatataaagtttatcccacc
gaaaatattattaaggatgtttgccaagagtttggtgtggaatataaagtttatcccacc
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ttcaaagcggcgatcgcctctaactatcgctggctagaggccatgggcaaagcatcgtga
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ttcaaagcggcgatcgcctctaactatcgctggctagaggccatgggcaaagcatcgtga</p></div>
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     atgaaggtcatcgtcgcttctggcccggacggtgcccgcaagcacgaggtggagctggca
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     <div class="span5"><p><font size="1">atgaaggtcatcgtcgcttctggcccggacggtgcccgcaagcacgaggtggagctggca
gccaacgccacgctcgcagatctgaagaaggcctaccaacggggtgtggacgtgcaccgc
gccaacgccacgctcgcagatctgaagaaggcctaccaacggggtgtggacgtgcaccgc
aagtcgttcaaggttcccagcgcggagtcgccgctgccaggtgcggatagtggcaagctg
aagtcgttcaaggttcccagcgcggagtcgccgctgccaggtgcggatagtggcaagctg
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acaaatatgttatcctcctggttcttcacactcgccggtttcgtgcagatggcggactgg
acaaatatgttatcctcctggttcttcacactcgccggtttcgtgcagatggcggactgg
gcgaagaagaagcaccggggctacgtcaaggcggacccggccaataaaaagaaggccgcc
gcgaagaagaagcaccggggctacgtcaaggcggacccggccaataaaaagaaggccgcc
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attctgcccttcatcatgtag
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attctgcccttcatcatgtag</p></div>
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Revision as of 11:05, 25 July 2012

Lab Book

Procedure

anyone really keen to write this up, up to protein visualization? Don't forget to mention the differing restriction enzymes and vectors of each team. I can add in links to the protocols page.

Please also refer to our Protocols page.

Start from lipid extraction

primers, sequence results

Sequences
Δ12 T. Cruzi
Δ12 Synechocystis
Δ15 Synechocystis
Δ6 Synechocystis
ELO 6 L. major

 

Primers
 
date
what we did

who knows what those Metal Mickies are up to. Eating nachos in the Whey Pat most likely.

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University of St Andrews, 2012.

Contact us: igem2012@st-andrews.ac.uk, Twitter, Facebook

This iGEM team has been funded by the MSD Scottish Life Sciences Fund. The opinions expressed by this iGEM team are those of the team members and do not necessarily represent those of Merck Sharp & Dohme Limited, nor its Affiliates.