Team:Exeter/lab book/gibs/wk1

From 2012.igem.org

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     <!12.7.12 <p>Chose three colonies from each plate.</p>
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     <!<p>12.7.12</p> <p>Chose three colonies from each plate.</p>
<p>Transferred aseptically into 1:1000 ampicillin:LB broth.</p> <p>Incubated at 37*C, 220rpm overnight.</p><p>13.7.12</p>
<p>Transferred aseptically into 1:1000 ampicillin:LB broth.</p> <p>Incubated at 37*C, 220rpm overnight.</p><p>13.7.12</p>
<p>Cultures were transferred to identical sterile falcon tubes and centrifuged at 21 C, 3900 rcf, for 2 minutes.</p>
<p>Cultures were transferred to identical sterile falcon tubes and centrifuged at 21 C, 3900 rcf, for 2 minutes.</p>

Revision as of 08:25, 25 July 2012

ExiGEM2012 Lab Book Gibs wk1

Operon Construction: 9th - 13th July 2012

 12.7.12

Chose three colonies from each plate.

Transferred aseptically into 1:1000 ampicillin:LB broth.

Incubated at 37*C, 220rpm overnight.

13.7.12

Cultures were transferred to identical sterile falcon tubes and centrifuged at 21 C, 3900 rcf, for 2 minutes.

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