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From 2012.igem.org

Revision as of 16:55, 8 July 2012 (view source) Emeraldo88 (Talk | contribs) ← Older edit		Revision as of 10:29, 24 July 2012 (view source) Elbrich (Talk | contribs) Newer edit →
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	3.)Plan for single enzyme restriction.		3.)Plan for single enzyme restriction.
	4.)The B. subtilis transformation is postponed		4.)The B. subtilis transformation is postponed
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		+	Elbrich
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		+	Minced Meat test
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		+	Yesterday we put the minced meat at 17.15 out of the fridge. Today I tested its freshess based on a smell scale (from 0-10) and physical appearance by taking a picture.
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		+	11.00 Picture and strong butter like smell. Grade 3 ~ smell not appealing at all, strong butterlike
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		+	12.02 Picture and smell grade 3
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		+	13.03 Picture and smell grade 3
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		+	14.03 Picture and smell grade 4 ~ butter smell increased
		+
		+	15.02 Smell grade 6 ~ butter smell transformed into more acid like smell
		+
		+	16.15 Smell grade 10 ~ disgusting

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Revision as of 10:29, 24 July 2012

Tom

Plasmid issolation of BBa_J37034 (LuxI added to GFP) and BBa_R0062 (promotor of LuxR (PR)).

Concentrations were low: LuxR (1) = 12.7 ng/ul, LuxR (2) = 25.2 ng/ul, LuxI (1) = 24.8 ng/ul, LuxI (2) = 22.3 ng/ul.

Colonies containing LuxI and LuxR were incubated in LB overnight.

Nisa and Emeraldo 1.)Plasmid isolation from O/N E. coli with BBa_K090403 gave a low yield (<30ng/ul) 2.)Restriction of BBa_K090403 and the pigments with EcoRI and SpeI failed, no multiple bands detected on gel 3.)Plan for single enzyme restriction. 4.)The B. subtilis transformation is postponed

Elbrich

Minced Meat test

Yesterday we put the minced meat at 17.15 out of the fridge. Today I tested its freshess based on a smell scale (from 0-10) and physical appearance by taking a picture.

11.00 Picture and strong butter like smell. Grade 3 ~ smell not appealing at all, strong butterlike

12.02 Picture and smell grade 3

13.03 Picture and smell grade 3

14.03 Picture and smell grade 4 ~ butter smell increased

15.02 Smell grade 6 ~ butter smell transformed into more acid like smell

16.15 Smell grade 10 ~ disgusting