Team:WashU/Week9
From 2012.igem.org
(Difference between revisions)
BrianBasco (Talk | contribs) |
BrianBasco (Talk | contribs) |
||
Line 10: | Line 10: | ||
Today we sonicated three cultures of <i>E. coli</i> - one with our Z construct, one with Z, C and inducers, and one with Z, C, but no inducer. Then, we extracted the soluble proteins from those cultures and ran them on a gel. [PICTURE] | Today we sonicated three cultures of <i>E. coli</i> - one with our Z construct, one with Z, C and inducers, and one with Z, C, but no inducer. Then, we extracted the soluble proteins from those cultures and ran them on a gel. [PICTURE] | ||
+ | |||
+ | We also ran a PCR of a series of ligations. The color constructs were ligated with promoters into chloramphenicol-resistant plasmid Psb1C3. [EXPAND UPON THIS] | ||
<br> | <br> |
Revision as of 18:04, 23 July 2012