Morning: Western blot, part 1
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Protocol: Western blot
#Casting the gels Gel percentage will depend on the size of the protein
4-40 kDA | 20% |
12-45kDA | 15% |
10-70 kDA | 12.5% |
15-100kDA | 10% |
25-200 kDA | 8%% |
Separating gel | |
Gel percentage | 7.5 % |
30% Polyacrylamide | 10 mL |
1.5M Tris (pH 8.8) | 10 mL |
10% Ammonium persulfate | 0.4 mL |
10% SDS | 0.4 mL |
TEMED | 0.038 mL |
H2O | 19.2 mL |
Total volume | 40 mL |
Stacking gel | |
Gel percentage | 5 % |
30% Polyacrylamide | 1.36 mL |
1M Tris (pH 6.8) | 1 mL |
10% Ammonium persulfate | 0.08 mL |
10% SDS | 0.08 mL |
TEMED | 0.008 mL |
H2O | 5.44 mL |
Total volume | 8 mL |
protocol here
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