Team:EPF-Lausanne/Notebook/23 July 2012

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== Western blot ==
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== Morning: Western blot, part 1 ==
{{:Team:EPF-Lausanne/Template/LabPresence|Alexandra, Sander, Andrei, Mouna}}
{{:Team:EPF-Lausanne/Template/LabPresence|Alexandra, Sander, Andrei, Mouna}}

Revision as of 11:51, 23 July 2012



Morning: Western blot, part 1

bladi blah


Protocol: Western blot

#Casting the gels Gel percentage will depend on the size of the protein

4-40 kDA 20%
12-45kDA 15%
10-70 kDA 12.5%
15-100kDA 10%
25-200 kDA 8%%


Separating gel
Gel percentage 7.5 %
30% Polyacrylamide 10 mL
1.5M Tris (pH 8.8) 10 mL
10% Ammonium persulfate 0.4 mL
10% SDS 0.4 mL
TEMED 0.038 mL
H2O 19.2 mL
Total volume 40 mL
Stacking gel
Gel percentage 5 %
30% Polyacrylamide 1.36 mL
1M Tris (pH 6.8) 1 mL
10% Ammonium persulfate 0.08 mL
10% SDS 0.08 mL
TEMED 0.008 mL
H2O 5.44 mL
Total volume 8 mL


protocol here


Comments

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