Team:ETH Zurich/Layout

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/* Author: Pieter van Boheemen */
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/* Team: TU Delft */
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=== E.colipse ===  
=== E.colipse ===  
===== Who’s your pABA ? =====
===== Who’s your pABA ? =====
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|Our system could be integrated into bacteria living on human skin and therefore serve as an adaptive, intelligent sunscreen that protects the skin upon UV light exposure and also tells when it is time to avoid the sun.
|Our system could be integrated into bacteria living on human skin and therefore serve as an adaptive, intelligent sunscreen that protects the skin upon UV light exposure and also tells when it is time to avoid the sun.
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<center>We're about to fill our Wiki and to provide all the required pages. Stay tuned for updates.</center>
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{{:Team:ETH_Zurich/Templates/Footer}}

Latest revision as of 18:05, 18 July 2012

Eth page header.png
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E.colipse

Who’s your pABA ?
We build a biological UV warning system in E.coli. The bacteria are able to detect UV light and can integrate the duration of their exposure to it.
UV-light exposure induces the production of para-Aminobenzoic acid (pABA), a common ingredient of sunscreen, as a protection against UV radiation damage. If the system is exposed to UV light for a duration sufficiently long or intense to be harmful to human skin, the cells produce a warning signal. As to our knowledge there is no specific UV light receptor in E.coli, we model and implement a spectral filter system out of different light receptors that responds only to UV light.
Our system could be integrated into bacteria living on human skin and therefore serve as an adaptive, intelligent sunscreen that protects the skin upon UV light exposure and also tells when it is time to avoid the sun.




We're about to fill our Wiki and to provide all the required pages. Stay tuned for updates.


References

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  • Christie, J. M., Salomon, M., Nozue, K., Wada, M., & Briggs, W. R. (1999): LOV (light, oxygen, or voltage) domains of the blue-light photoreceptor phototropin (nph1): binding sites for the chromophore flavin mononucleotide. Proceedings of the National Academy of Sciences of the United States of America, 96(15), 8779–83.
  • Christie, J. M., Arvai, A. S., Baxter, K. J., Heilmann, M., Pratt, A. J., O’Hara, A., Kelly, S. M., et al. (2012). Plant UVR8 photoreceptor senses UV-B by tryptophan-mediated disruption of cross-dimer salt bridges. Science (New York, N.Y.), 335(6075), 1492–6.
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  • Tschowri, N., Lindenberg, S., & Hengge, R. (2012). Molecular function and potential evolution of the biofilm-modulating blue light-signalling pathway of Escherichia coli. Molecular microbiology.
  • Tyagi, A. (2009). Photodynamics of a flavin based blue-light regulated phosphodiesterase protein and its photoreceptor BLUF domain.
  • Vainio, H. & Bianchini, F. (2001). IARC Handbooks of Cancer Prevention: Volume 5: Sunscreens. Oxford University Press, USA
  • Quinlivan, Eoin P & Roje, Sanja & Basset, Gilles & Shachar-Hill, Yair & Gregory, Jesse F & Hanson, Andrew D. (2003). The folate precursor p-aminobenzoate is reversibly converted to its glucose ester in the plant cytosol. The Journal of biological chemistry, 278.
  • van Thor, J. J., Borucki, B., Crielaard, W., Otto, H., Lamparter, T., Hughes, J., Hellingwerf, K. J., et al. (2001). Light-induced proton release and proton uptake reactions in the cyanobacterial phytochrome Cph1. Biochemistry, 40(38), 11460–71.
  • Wegkamp A, van Oorschot W, de Vos WM, Smid EJ. (2007 )Characterization of the role of para-aminobenzoic acid biosynthesis in folate production by Lactococcus lactis. Appl Environ Microbiol. Apr;73(8):2673-81.