"
Template:Team:Edinburgh/Notebook/Week04
From 2012.igem.org
| Line 24: | Line 24: | ||
Control – IPTG <br/> | Control – IPTG <br/> | ||
The MTZ plates were streaked with either BS control or BS nitred. All other plates were streaked with the four nitroreductase strains. <br/> | The MTZ plates were streaked with either BS control or BS nitred. All other plates were streaked with the four nitroreductase strains. <br/> | ||
| + | div id="week04"> | ||
| + | <div class="edi-lab-book-entry" id="w04-17-07"> | ||
| + | <div class="entry-title"> | ||
| + | <p><a name="week04-17-07">Week 4, 17.07.2012, Tuesday</a></p> | ||
| + | </div> | ||
| + | <div class="entry-details"> | ||
| + | <p>Posted on 17/07/2012</p> | ||
| + | </div> | ||
| + | <div class="entry-text"> | ||
| + | <p> | ||
Revision as of 14:57, 18 July 2012
Posted on 16/07/2012
1) The M9 agar plates were inoculated with C.freundii SBS 197, C.freundii NCIMB, E.coli J109, E.coli + sucrose hydrolase. The plates were left o/n 37ºC.
2) LB plates with 200 µl carbinicillin were prepared:
2* 50 µl MTZ (out of 50 mg/ml stock) 100 µg/ml+ 25 µg/ml IPTG
2*45 µl MTZ (out of 50 mg/ml stock) 90 µg/ml+ 25 µg/ml IPTG
5 µl NFT (out of 5 mg/ml stock) 1 µg/ml +25 µg/ml IPTG
1 µl NFT (out of 5 mg/ml stock) 0.2 µg/ml +25 µg/ml IPTG
50 µl DNBA (out of 50 mg/ml stock) 100 µg/ml +25 µg/ml IPTG
75 µl DNBA (out of 50 mg/ml stock) 150 µg/ml +25 µg/ml IPTG
Control +25 µg/ml IPTG
Control – IPTG
The MTZ plates were streaked with either BS control or BS nitred. All other plates were streaked with the four nitroreductase strains.
div id="week04">
