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| + | <b>1) The M9 agar plates were inoculated with C.freundii SBS 197, C.freundii NCIMB, E.coli J109, E.coli + sucrose hydrolase. The plates were left o/n 37ºC. </b> <br/> | ||
| + | |||
| + | <b> 2) LB plates with 200 µl carbinicillin were prepared: </b> <br/> | ||
| + | 2* 50 µl MTZ (out of 50 mg/ml stock) 100 µg/ml+ 25 µg/ml IPTG <br/> | ||
| + | 2*45 µl MTZ (out of 50 mg/ml stock) 90 µg/ml+ 25 µg/ml IPTG <br/> | ||
| + | 5 µl NFT (out of 5 mg/ml stock) 1 µg/ml +25 µg/ml IPTG <br/> | ||
| + | 1 µl NFT (out of 5 mg/ml stock) 0.2 µg/ml +25 µg/ml IPTG <br/> | ||
| + | 50 µl DNBA (out of 50 mg/ml stock) 100 µg/ml +25 µg/ml IPTG <br/> | ||
| + | 75 µl DNBA (out of 50 mg/ml stock) 150 µg/ml +25 µg/ml IPTG <br/> | ||
| + | Control +25 µg/ml IPTG <br/> | ||
| + | Control – IPTG <br/> | ||
| + | The MTZ plates were streaked with either BS control or BS nitred. All other plates were streaked with the four nitroreductase strains. <br/> | ||
| + | |||
</p> | </p> | ||
Revision as of 14:56, 18 July 2012
Posted on 16/07/2012
1) The M9 agar plates were inoculated with C.freundii SBS 197, C.freundii NCIMB, E.coli J109, E.coli + sucrose hydrolase. The plates were left o/n 37ºC.
2) LB plates with 200 µl carbinicillin were prepared:
2* 50 µl MTZ (out of 50 mg/ml stock) 100 µg/ml+ 25 µg/ml IPTG
2*45 µl MTZ (out of 50 mg/ml stock) 90 µg/ml+ 25 µg/ml IPTG
5 µl NFT (out of 5 mg/ml stock) 1 µg/ml +25 µg/ml IPTG
1 µl NFT (out of 5 mg/ml stock) 0.2 µg/ml +25 µg/ml IPTG
50 µl DNBA (out of 50 mg/ml stock) 100 µg/ml +25 µg/ml IPTG
75 µl DNBA (out of 50 mg/ml stock) 150 µg/ml +25 µg/ml IPTG
Control +25 µg/ml IPTG
Control – IPTG
The MTZ plates were streaked with either BS control or BS nitred. All other plates were streaked with the four nitroreductase strains.
